The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties

Siddique, Masood; Gernhard, Sascha; Koskull‐Döring, Pascal von; Vierling, Elizabeth; Scharf, Klaus‐Dieter

doi:10.1007/s12192-008-0032-6

Cited by 216 publications

(239 citation statements)

References 75 publications

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“…HSPs play crucial roles in protein refolding, assembly, trafficking, stability, and degradation and in preventing protein aggregation under stress and nonstress conditions (Scharf et al, 2001;Wang et al, 2004;Siddique et al, 2008;Bondino et al, 2012;Waters, 2013). The Arabidopsis genome contains 19 classical sHSPs, which are divided into 11 subfamilies according to their intracellular localization (Scharf et al, 2001;Siddique et al, 2008;Waters, 2013).…”

Section: Discussionmentioning

confidence: 99%

REPRESSOR OF SILENCING5 Encodes a Member of the Small Heat Shock Protein Family and Is Required for DNA Demethylation in Arabidopsis

Zhao

Xie

et al. 2014

The Plant Cell

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In Arabidopsis thaliana, active DNA demethylation is initiated by the DNA glycosylase REPRESSOR OF SILENCING1 (ROS1) and its paralogs DEMETER, DEMETER-LIKE2 (DML2), and DML3. How these demethylation enzymes are regulated, however, is poorly understood. Here, using a transgenic Arabidopsis line harboring the stress-inducible RESPONSIVE TO DEHYDRATION29A (RD29A) promoter-LUCIFERASE (LUC) reporter gene and the cauliflower mosaic virus 35S promoter (35S)-NEOMYCIN PHOSPHOTRANSFERASE II (NPTII) antibiotic resistance marker gene, we characterize a ROS locus, ROS5, that encodes a protein in the small heat shock protein family. ROS5 mutations lead to the silencing of the 35S-NPTII transgene due to DNA hypermethylation but do not affect the expression of the RD29A-LUC transgene. ROS5 physically interacts with the histone acetyltransferase ROS4/INCREASED DNA METHYLATION1 (IDM1) and is required to prevent the DNA hypermethylation of some genes that are also regulated by ROS1 and IDM1. We propose that ROS5 regulates DNA demethylation by interacting with IDM1, thereby creating a chromatin environment that facilitates the binding of ROS1 to erase DNA methylation.

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Section: Discussionmentioning

confidence: 99%

REPRESSOR OF SILENCING5 Encodes a Member of the Small Heat Shock Protein Family and Is Required for DNA Demethylation in Arabidopsis

Zhao

Xie

et al. 2014

The Plant Cell

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“…During embryo development, Br15.5 IV and Br20.7 V were weakly expressed at the three-embryo stage, whereas Br15.8 IV was up-regulated during embryo development. The corresponding sHSP genes of Arabidopsis (At21.7 V) are expressed constitutively in vegetative organs (Siddique et al, 2008). All organellar sHSP genes of Chinese cabbage showed dominant expression in siliques and in early cotyledon embryos.…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis thaliana, more than seven different sHSP subfamilies have been identified by genome analysis (Scharf et al, 2001). Six cytosolic-I genes constitute the largest sHSP subfamily in A. thaliana (Siddique et al, 2008;Waters et al, 2008). Comparative analysis of A. thaliana, Populus trichocarpa, and Oryza sativa (rice), has revealed one additional mitochondrial and three additional cytosolic sHSP subfamilies (Waters et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Peng

Guo²,

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Small heat shock proteins (sHSPs) are essential for the plant's normal development and stress responses, especially the heat stress response. The information regarding sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis) is sparse, hence we performed a genome-wide analysis to identify sHSP genes in this species. We identified 26 non-redundant sHSP genes distributed on all chromosomes, except chromosome A7, with one additional sHSP gene identified from an expressed sequence tag library. Chinese cabbage was found to contain more sHSP genes than Arabidopsis. The 27 sHSP genes were classified into 11 subfamilies. We identified 22 groups of sHSP syntenic orthologous genes between Chinese cabbage and Arabidopsis. In addition, eight groups of paralogous genes were uncovered in Chinese cabbage. Protein structures of the 27 Chinese cabbage sHSPs were modeled using Phyre2, which revealed that all of them contain several conserved β strands across different subfamilies. In general, gene structure was conserved within each subfamily between P. Tao et al.

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“…An extraction buffer, described by Siddique et al (2008), was added in a 1:2 ratio (plant tissue:buffer) Total protein content was quantified with a BCA Protein Assay (Pierce). SDS-PAGE was performed on a 10% Criterion polyacrylamide gel (Bio-Rad Laboratories).…”

Section: Immunoblottingmentioning

confidence: 99%

The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis

et al. 2010

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Anoxia induces several heat shock proteins, and a mild heat pretreatment can acclimatize Arabidopsis (Arabidopsis thaliana) seedlings to subsequent anoxic treatment. In this study, we analyzed the response of Arabidopsis seedlings to anoxia, heat, and combined heat + anoxia stress. A significant overlap between the anoxic and the heat responses was observed by wholegenome microarray analysis. Among the transcription factors induced by both heat and anoxia, the heat shock factor A2 (HsfA2), known to be involved in Arabidopsis acclimation to heat and to other abiotic stresses, was strongly induced by anoxia. Heat-dependent acclimation to anoxia is lost in an HsfA2 knockout mutant (hsfa2) as well as in a double mutant for the constitutively expressed HsfA1a/HsfA1b (hsfA1a/1b), indicating that these three heat shock factors cooperate to confer anoxia tolerance. Arabidopsis seedlings that overexpress HsfA2 showed an increased expression of several known targets of this transcription factor and were markedly more tolerant to anoxia as well as to submergence. Anoxia failed to induce HsfA2 target proteins in wild-type seedlings, while overexpression of HsfA2 resulted in the production of HsfA2 targets under anoxia, correlating well with the low anoxia tolerance experiments. These results indicate that there is a considerable overlap between the molecular mechanisms of heat and anoxia tolerance and that HsfA2 is a player in these mechanisms.

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The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties

Abstract: The small heat shock proteins (sHsps

Cited by 216 publications

References 75 publications

REPRESSOR OF SILENCING5 Encodes a Member of the Small Heat Shock Protein Family and Is Required for DNA Demethylation in Arabidopsis

REPRESSOR OF SILENCING5 Encodes a Member of the Small Heat Shock Protein Family and Is Required for DNA Demethylation in Arabidopsis

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis

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