The Plant-Specific ssDNA Binding Protein OSB1 Is Involved in the Stoichiometric Transmission of Mitochondrial DNA in<i>Arabidopsis</i>

Zaegel, Vincent; Guermann, Benoît; Ret, Monique Le; Andres, C. J.; Meyer, Denise; Erhardt, Mathieu; Canaday, Jean; Gualberto, José M.; Imbault, P.

doi:10.1105/tpc.106.042028

Cited by 127 publications

(160 citation statements)

References 47 publications

Supporting

Mentioning

155

Contrasting

Order By: Relevance

“…Mutation of MutS homolog 1 (MSH1), which encodes a homolog of E. coli MutS targeted to mitochondria, confers an accumulation of two kinds of mitochondrial DNA: one product is generated by aberrant recombination between two 249-bp repeats; another is generated by recombination between two 335-bp repeats (Abdelnoor et al, 2003;Shedge et al, 2007). A similar accumulation of recombined mitochondrial DNA molecules has been observed in an Organellar Single-stranded DNA Binding protein 1 (OSB1) mutant of Arabidopsis (Zaegel et al, 2006). In this mutant, four pairs of repeats were reported to be involved in the recombination event: a pair of 435-bp repeats and a pair of 556-bp repeats as well as the two repeat pairs that were studied in the MSH1 mutant described above.…”

Section: Discussionmentioning

confidence: 63%

Suppression of Repeat-Mediated Gross Mitochondrial Genome Rearrangements by RecA in the Moss Physcomitrella patens

Odahara

Kuroiwa

et al. 2009

The Plant Cell

View full text Add to dashboard Cite

RecA and its ubiquitous homologs are crucial components in homologous recombination. Besides their eukaryotic nuclear counterparts, plants characteristically possess several bacterial-type RecA proteins localized to chloroplasts and/or mitochondria, but their roles are poorly understood. Here, we analyzed the role of the only mitochondrial RecA in the moss Physcomitrella patens. Disruption of the P. patens mitochondrial recA gene RECA1 caused serious defects in plant growth and development and abnormal mitochondrial morphology. Analyses of mitochondrial DNA in disruptants revealed that frequent DNA rearrangements occurred at multiple loci. Structural analysis suggests that the rearrangements, which in some cases were associated with partial deletions and amplifications of mitochondrial DNA, were due to aberrant recombination between short (<100 bp) direct and inverted repeats in which the sequences were not always identical. Such repeats are abundant in the mitochondrial genome, and interestingly many are located in group II introns. These results suggest that RECA1 does not promote but rather suppresses recombination among short repeats scattered throughout the mitochondrial genome, thereby maintaining mitochondrial genome stability. We propose that RecA-mediated homologous recombination plays a crucial role in suppression of short repeat-mediated genome rearrangements in plant mitochondria.

show abstract

Section: Discussionmentioning

confidence: 63%

Suppression of Repeat-Mediated Gross Mitochondrial Genome Rearrangements by RecA in the Moss Physcomitrella patens

Odahara

Kuroiwa

et al. 2009

The Plant Cell

View full text Add to dashboard Cite

show abstract

“…This insertion was suggested to involve a 9-bp repeat-mediated integration. The sequence in question represents a recombinationally active region (Zaegel et al 2006;Shedge et al 2007). Similarly, mitochondrial genomic environments present in Col-0 have seemingly disappeared from the Ler genome (Figure 6).…”

Section: Mitochondrial Genome Comparisons In Arabidopsismentioning

confidence: 99%

“…Three nuclear genes have been shown to influence recombination within the plant mitochondrial genome: MSH1 (Abdelnoor et al 2003), RECA3 (Shedge et al 2007), and OSB1 (Zaegel et al 2006). Of these, MSH1 appears to have the most profound and immediate effect on the genome and on plant phenotype (Sandhu et al 2007;Shedge et al 2007).…”

Section: T He Plant Mitochondrial Genome Is Characterizedmentioning

confidence: 99%

Diversity of the Arabidopsis Mitochondrial Genome Occurs via Nuclear-Controlled Recombination Activity

Arrieta-Montiel¹,

Shedge²,

Davila³

et al. 2009

Genetics

169

217

View full text Add to dashboard Cite

The plant mitochondrial genome is recombinogenic, with DNA exchange activity controlled to a large extent by nuclear gene products. One nuclear gene, MSH1, appears to participate in suppressing recombination in Arabidopsis at every repeated sequence ranging in size from 108 to 556 bp. Present in a wide range of plant species, these mitochondrial repeats display evidence of successful asymmetric DNA exchange in Arabidopsis when MSH1 is disrupted. Recombination frequency appears to be influenced by repeat sequence homology and size, with larger size repeats corresponding to increased DNA exchange activity. The extensive mitochondrial genomic reorganization of the msh1 mutant produced altered mitochondrial transcription patterns. Comparison of mitochondrial genomes from the Arabidopsis ecotypes C24, Col-0, and Ler suggests that MSH1 activity accounts for most or all of the polymorphisms distinguishing these genomes, producing ecotype-specific stoichiometric changes in each line. Our observations suggest that MSH1 participates in mitochondrial genome evolution by influencing the lineagespecific pattern of mitochondrial genetic variation in higher plants.

show abstract

“…Expression of the mRNAs was tested by amplification of a cDNA fragment spanning the insertion site of the T-DNA, using primers P5 and P6. Total plant RNA and genomic DNA were extracted with TRIzol and DNAzol (Invitrogen), respectively, as described (Zaegel et al, 2006).…”

Section: Methods Plant Materials and Growth Conditionsmentioning

confidence: 99%

Arabidopsis tRNA Adenosine Deaminase Arginine Edits the Wobble Nucleotide of Chloroplast tRNAArg(ACG) and Is Essential for Efficient Chloroplast Translation

et al. 2009

Self Cite

View full text Add to dashboard Cite

RNA editing changes the coding/decoding information relayed by transcripts via nucleotide insertion, deletion, or conversion. Editing of tRNA anticodons by deamination of adenine to inosine is used both by eukaryotes and prokaryotes to expand the decoding capacity of individual tRNAs. This limits the number of tRNA species required for codon-anticodon recognition. We have identified the Arabidopsis thaliana gene that codes for tRNA adenosine deaminase arginine (TADA), a chloroplast tRNA editing protein specifically required for deamination of chloroplast (cp)-tRNA Arg (ACG) to cp-tRNA Arg (ICG). Land plant TADAs have a C-terminal domain similar in sequence and predicted structure to prokaryotic tRNA deaminases and also have very long N-terminal extensions of unknown origin and function. Biochemical and mutant complementation studies showed that the C-terminal domain is sufficient for cognate tRNA deamination both in vitro and in planta. Disruption of TADA has profound effects on chloroplast translation efficiency, leading to reduced yields of chloroplast-encoded proteins and impaired photosynthetic function. By contrast, chloroplast transcripts accumulate to levels significantly above those of wild-type plants. Nevertheless, absence of cp-tRNA Arg (ICG) is compatible with plant survival, implying that two out of three CGN codon recognition occurs in chloroplasts, though this mechanism is less efficient than wobble pairing.

show abstract

The Plant-Specific ssDNA Binding Protein OSB1 Is Involved in the Stoichiometric Transmission of Mitochondrial DNA inArabidopsis

Cited by 127 publications

References 47 publications

Suppression of Repeat-Mediated Gross Mitochondrial Genome Rearrangements by RecA in the Moss Physcomitrella patens

Suppression of Repeat-Mediated Gross Mitochondrial Genome Rearrangements by RecA in the Moss Physcomitrella patens

Diversity of the Arabidopsis Mitochondrial Genome Occurs via Nuclear-Controlled Recombination Activity

Arabidopsis tRNA Adenosine Deaminase Arginine Edits the Wobble Nucleotide of Chloroplast tRNAArg(ACG) and Is Essential for Efficient Chloroplast Translation

Contact Info

Product

Resources

About