The plasma membrane as a mechanochemical transducer

Roux, Anabel‐Lise Le; Quiroga, Xarxa; Walani, Nikhil; Arroyo, Marino; Roca‐Cusachs, Pere

doi:10.1098/rstb.2018.0221

Cited by 155 publications

(132 citation statements)

References 195 publications

(337 reference statements)

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“…Cellular deformations induce adaptive signaling that provides adequate function, fulfilling the actual environmental needs/challenges. [22][23][24][25][26]. The opening of membrane channels followed by calcium influx or activation of enzymes such as adenylate or guanylate cyclase and the production of cyclic adenosine or guanosine monophosphate (cAMP or cGMP) are part of such intracellular signaling cascades.…”

Section: Mechanosensing and Mechanotransductionmentioning

confidence: 99%

Interactions between Muscle and Bone—Where Physics Meets Biology

et al. 2020

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Muscle and bone interact via physical forces and secreted osteokines and myokines. Physical forces are generated through gravity, locomotion, exercise, and external devices. Cells sense mechanical strain via adhesion molecules and translate it into biochemical responses, modulating the basic mechanisms of cellular biology such as lineage commitment, tissue formation, and maturation. This may result in the initiation of bone formation, muscle hypertrophy, and the enhanced production of extracellular matrix constituents, adhesion molecules, and cytoskeletal elements. Bone and muscle mass, resistance to strain, and the stiffness of matrix, cells, and tissues are enhanced, influencing fracture resistance and muscle power. This propagates a dynamic and continuous reciprocity of physicochemical interaction. Secreted growth and differentiation factors are important effectors of mutual interaction. The acute effects of exercise induce the secretion of exosomes with cargo molecules that are capable of mediating the endocrine effects between muscle, bone, and the organism. Long-term changes induce adaptations of the respective tissue secretome that maintain adequate homeostatic conditions. Lessons from unloading, microgravity, and disuse teach us that gratuitous tissue is removed or reorganized while immobility and inflammation trigger muscle and bone marrow fatty infiltration and propagate degenerative diseases such as sarcopenia and osteoporosis. Ongoing research will certainly find new therapeutic targets for prevention and treatment.

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Section: Mechanosensing and Mechanotransductionmentioning

confidence: 99%

Interactions between Muscle and Bone—Where Physics Meets Biology

et al. 2020

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“…The curvature sensing and membrane reshaping properties of BAR proteins have been extensively studied on highly curved tubes (up to 100 nm in diameter), mostly in equilibrium 4,29,30,31 . However, many cell studies pointed out the role of BAR proteins acting on lower curvature lipid structures 32 . Our study demonstrates and characterizes reshaping at low concentration, low curvature, and low tension, a highly relevant scenario in cells.…”

Section: Mainmentioning

confidence: 99%

Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization

Roux

Tozzi

Walani

et al. 2020

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In many physiological situations, BAR proteins interact with, and reshape, pre-existing curved membranes, contributing to essential cellular processes. However, the non-equilibrium and timedependent process of reshaping, and its dependence on initial membrane shape, remains largely unknown. Here we explain, both experimentally and through modelling, how a BAR protein dynamically interacts with mechanically bent lipid membranes. We capture protein binding to curved membranes, and characterize a variety of dynamical reshaping events depending on membrane shape and protein arrangement. The events can be generally understood by an isotropic-to-nematic phase transition, in which low curvature templates with isotropic protein orientation progress towards highly curved lipid tubes with nematic protein arrangement. Our findings also apply in cells, where mechanical stretch triggers BAR-protein-membrane interactions that enable potential mechanotransduction mechanisms. Our results characterize and broaden the reshaping processes of BAR proteins on mechanically constrained membranes, demonstrating the interplay between membrane mechanical stimuli and BAR protein response.

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“…Because muscle cells follow repeated cycles of elongation and relaxation, muscle cells are constantly exposed to important mechanical constraints (9) and are prone to experience membrane tension increases (10). Changes in membrane tension are known to be transduced into surface area and volume changes (11).…”

Section: Recapitulating the In Vivo Lipid Signature In Vitromentioning

confidence: 99%

Polyunsaturated phospholipids increase cell resilience to mechanical constraints

Kadri

Bâcle

Khoury

et al. 2020

Preprint

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Despite the fact that PolyUnsaturated Fatty Acids (PUFA) are generally accepted to be good for health, the mechanisms of their bona fide benefits still remain elusive. PUFA are particularly enriched in the membrane Phospholipids (PLs) of selective organs, among which skeletal muscles and the cardiovascular system are prime examples. The fatty acid composition of PLs is known to regulate crucial membrane properties, including rigidity, elasticity and plasticity. Since muscle cells undergo repeated cycles of elongation and relaxation and are constantly exposed to mechanical stress, we postulated in the present study that PUFA-containing PLs could be central players for muscle cell adaptation to mechanical constraints. By a combination of in cellulo and in silico approaches, we show that PUFA, and particularly the ω-3 DocoHexaenoic Acid (DHA), regulate important properties of the plasma membrane that improve muscle cell resilience to mechanical constraints. Thanks to their unique property to contortionate within the bilayer plane, they facilitate the formation of Vacuole-like dilation (VLD) which, in turn, reduce membrane stress and avoid cell breakage under mechanical constraints.

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The plasma membrane as a mechanochemical transducer

Cited by 155 publications

References 195 publications

Interactions between Muscle and Bone—Where Physics Meets Biology

Interactions between Muscle and Bone—Where Physics Meets Biology

Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization

Polyunsaturated phospholipids increase cell resilience to mechanical constraints

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