The plasma membrane H+-ATPase gene family in Solanum tuberosum L. Role of PHA1 in tuberization

Stritzler, Margarita; García, María Noelia Muñiz; Schlesinger, Mariana; Cortelezzi, Juan Ignacio; Capiati, Daniela Andrea

doi:10.1093/jxb/erx284

Cited by 24 publications

(20 citation statements)

References 75 publications

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“…Hence, PM H + -ATPase is believed to play a major role in salt stress tolerance [13]. In this study, the expression level of HHA4, HHA11, and HHA13 were increased under salt stress ( Figure 6), which was consistent with many previous studies that suggested that salt stress could induce the expression of PM H + -ATPases in plants [11,12,26,52]. The stress-induced expression level suggests that PM H + -ATPases might potentially place an important role in the development of salt tolerance.…”

Section: Discussionsupporting

confidence: 91%

“…Hence, they are vital for many life activities of organisms [3,16,43]. Until now, the members of PM H + -ATPases were identified in many species, such as in Arabidopsis, Oryza sativa, Lycopersicon esculentum, Zea mays, Cucumis sativus, Solanum tuberosum L, and Nicotiana plumbaginifolia [5,15,16,[26][27][28]. The recent release of a complete sunflower genome sequences and annotations provides convenience for PM H + -ATPase sub-gene family member identification and functional studies [29].…”

Section: Discussionmentioning

confidence: 99%

“…P-type PM H + -ATPases protein sequence multiple alignments of Helianthus annuus (HHA), A. thaliana (AHA), O. sativa (OSA), N. plumbaginifolia (PMA), and S. tuberosum Phureja (PHA) [26] were aligned by ClustalW program with a gap extension penalty of 0.1. Then, a phylogenetic tree was constructed using Mega 6.0 [33].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…For example, the PPI (proton pump interactor), which was identified in A. thaliana [24] and Solanum tuberosum [25], is a novel interaction partner of PM H + -ATPase. The activity of the proton pump was increased when it interacted with PPI at the C-terminus in vitro [26].…”

Section: Introductionmentioning

confidence: 99%

“…Previous genome-wide analysis studies reported that there are 10 and 11 PM H + -ATPase gene families in Oryza sativa and Arabidopsis thaliana, respectively [5]. There are also 12 in Lycopersicon esculentum [15], 4 in Zea mays [27], 10 in Cucumis sativus [28], 7 in Solanum tuberosum L [26], and 9 in Nicotiana plumbaginifolia [16]. Phylogenetic analysis further divided the PM H + -ATPase into five sub-clusters according to the predicted amino acid sequences [5,16].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Marowa

Liu

et al. 2020

Genes

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The P-type plasma membrane (PM) H+-ATPase plays a major role during the growth and development of a plant. It is also involved in plant resistance to a variety of biotic and abiotic factors, including salt stress. The PM H+-ATPase gene family has been well characterized in Arabidopsis and other crop plants such as rice, cucumber, and potato; however, the same cannot be said in sunflower (Helianthus annuus). In this study, a total of thirteen PM H+-ATPase genes were screened from the recently released sunflower genome database with a comprehensive genome-wide analysis. According to a systematic phylogenetic classification with a previously reported species, the sunflower PM H+-ATPase genes (HHAs) were divided into four sub-clusters (I, II, IV, and V). In addition, systematic bioinformatics analyses such as gene structure analysis, chromosome location analysis, subcellular localization predication, conserved motifs, and Cis-acting elements of promoter identification were also done. Semi-quantitative PCR analysis data of HHAs in different sunflower tissues revealed the specificity of gene spatiotemporal expression and sub-cluster grouping. Those belonging to sub-cluster I and II exhibited wide expression in almost all of the tissues studied while sub-cluster IV and V seldom showed expression. In addition, the expression of HHA4, HHA11, and HHA13 was shown to be induced by salt stress. The transgenic plants overexpressing HHA4 and HHA11 showed higher salinity tolerance compared with wild-type plants. Further analysis showed that the Na+ content of transgenic Arabidopsis plants decreased under salt stress, which indicates that PM H+ ATPase participates in the physiological process of Na+ efflux, resulting in salt resistance of the plants. This study is the first to identify and analyze the sunflower PM H+ ATPase gene family. It does not only lay foundation for future research but also demonstrates the role played by HHAs in salt stress tolerance.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Marowa

Liu

et al. 2020

Genes

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Potato tuber degradation is regulated by carbohydrate metabolism: Results of transcriptomic analysis

et al. 2022

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Tuber number is an essential factor determining yield and commodity in potato production. The initiation number has long been considered the sole determinant of the final total tuber number. In this study, we observed that tuber numbers at harvest were lower than at the tuber bulking stage; some formed tubers that were smaller than 3 cm degraded during development. Carbohydrate metabolism plays a crucial role in tuber degradation by coordinating the source–sink relationship. The contents of starch and sucrose, and the C:N ratio, are dramatically reduced in degradating tubers. Transcriptomic study showed that “carbohydrate metabolic processes” are Gene Ontology (GO) terms associated with tuber degradation. A polysaccharide degradation‐related gene, LOC102601831, and a sugar transport gene, LOC102587850 (SWEET6a), are dramatically up‐regulated in degradating tubers according to transcriptomic analysis, as validated by qRT‐PCT. The terms “peptidase inhibitor activity” and “hydrolase activity” refer to the changes in molecular functions that degradating tubers exhibit. Nitrogen supplementation during potato development alleviates tuber degradation to a certain degree. This study provides novel insight into potato tuber development and possible management strategies for improving potato cultivation.

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Seed Germination: Explicit Crosstalk Between Hormones and ROS

Majumdar

Kar

2021

Plant in Challenging Environments

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The plasma membrane H+-ATPase gene family in Solanum tuberosum L. Role of PHA1 in tuberization

Abstract: Genetic evidence is provided for the role of potato PM H+-ATPases in tuberization, demonstrating that the isoform PHA1 promotes stolon elongation and tuber growth, and is involved in sugar–starch metabolism.

Cited by 24 publications

References 75 publications

Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Potato tuber degradation is regulated by carbohydrate metabolism: Results of transcriptomic analysis

Seed Germination: Explicit Crosstalk Between Hormones and ROS

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