The Plasma Preincubation with Papain Before the Assay Suggests that a Gell and Coombs Type II Reaction is Been Demonstrated by the Leukocyte Adherence Inhibition Test

Olivier, Celso Eduardo; Lima, Regiane Patussi dos Santos; Pinto, Daiana G.; Santos, Raquel Acácia Pereira Gonçalves dos

doi:10.26717/bjstr.2021.36.005869

Cited by 4 publications

(4 citation statements)

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“…When functionally activated by specific antigens, leukocytes release paracrine soluble factors that interfere with glass adherence of nearby leukocytes, a nonspecific phenomenon, acting just as the final indicator of the specific leukocyte immunoreactivity that can be quantified with a concomitant assay done with unchallenged plasma [41]- [45]. Besides the cellular involvement, the adherence inhibition phenomena also may require the participation of specific IgG antibodies, suggesting a type II Gell and Coombs antibody-dependent cellular-mediated immune response [46]- [48]. The LAIT or the quantification of allergen-specific IgG antibodies against food allergens are not clinically recognized diagnostic assays, however, they may be proposed as triage tests to reduce the empiricism of the prescribed exclusion diets before initiating a diagnostic OFC [49]- [53].…”

Section: Discussionmentioning

confidence: 99%

Leukocyte Adherence Inhibition Test to the assessment of Immunoreactivity Against Cow’s Milk Proteins in Non—IgE-Mediated Gastrointestinal Food Allergy

et al. 2022

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Background: The non—IgE-mediated food allergy syndromes are a group of conditions diagnosed mostly by clinical criteria and Oral Food Challenge tests (OFC). Objective: To evaluate the feasibility of the Leukocyte Adherence Inhibition Test (LAIT) to discriminate antigen-specific immunoreactivity in a group of patients with non—IgE-mediated cow’s milk allergy diagnosed by OFC and clinical criteria. Methods: Ex vivo challenge tests performed with cow’s milk proteins extracts were monitored by LAIT in two groups: A) the active group consisting of 38 patients presenting non—IgE-mediated gastrointestinal lactose-free cow’s milk hypersensitivity; B) the control group consisting of 29 cow’s milk tolerant individuals Results: The mean Leukocyte Adherence Inhibition (LAI) of the control group was 5.48%, and the mean LAI of the active group was 54.18%. The non-parametric Wilcoxon-Mann-Whitney U test showed p < α = 0.05. Conclusion: The Leukocyte Adherence Inhibition Test is an ex vivo immunoassay able to predict individual non—IgE-mediated immunoreactivity against cow’s milk allergens and to act as a triage test to select food allergens to be prescribed in exclusion diets to perform OFC in patients with suspected non—IgE-mediated food allergies.

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Section: Discussionmentioning

confidence: 99%

Leukocyte Adherence Inhibition Test to the assessment of Immunoreactivity Against Cow’s Milk Proteins in Non—IgE-Mediated Gastrointestinal Food Allergy

et al. 2022

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“…When put in contact with an immunoreactive antigen, leukocytes lost their ability to adhere to glass, showing a non-specific immunoreactivity against this specific substance [30]. Several mediators were already described as involved in this immunoassay, suggesting that a Gell & Coombs type II reaction is at play [31]- [33]. In this descriptive work, we compile the data supplied by the LAIT performed with fresh blood of "intrinsic" asthmatic outpatients to provide an overview of their relative non-IgE-mediated immunoreactivity against some common allergens.…”

Section: Introductionmentioning

confidence: 93%

Evaluating Non-IgE-Mediated Allergens’ Immunoreactivity in Patients Formerly Classified as “Intrinsic” Asthmatics with Help of the Leukocyte Adherence Inhibition Test

Olivier¹,

Pinto²,

Teixeira³

et al. 2023

CLINICMED

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Background: The ancient concept of “intrinsic” and “extrinsic” asthma has evolved in line with growing scientific knowledge and the use of new clinical diagnostic tools. Objective: The present study aims to evaluate the non-IgE-mediated immunoreactivity against common allergens, in asthmatic patients without evidence of IgE-mediated hypersensitivity against these allergens. Methods: A group of 127 “intrinsic” asthmatic outpatients, were submitted to ex vivo Leukocyte Adherence Inhibition Tests (LAIT) with extracts of Dermatophagoides pteronyssinus, Hevea brasiliensis latex, dog dander, cat dander, cow’s milk proteins, beekeeping pollen and a mixture of fungal extracts. Results: Cascade graphs were assembled according to the distribution of the LAIT results among the range of results of Leukocyte Adherence Inhibition (LAI) inside each group. A column graph was plotted with the mean LAI results for each antigen. The non-parametric Wilcoxon-Mann-Whitney U-test performed between the results of each test was significant only between the results of the LAIT performed with the dog extract and the mixed fungal extract (U = 1,179.5; the z-score was -2.64109; the p-value was 0.0083). All other Wilcoxon-Mann-Whitney U-test calculations between LAIT performed with the other allergens were not significant at p < 0.01. Conclusion: The evidence of non-IgE-mediated immunoreactivity in allergic patients has been reported in the medical literature since the beginning of the 19th century and has been increasing since then. This descriptive article presents the contribution of the LAIT as a tool to quantify the overall non-IgE-mediated cellular/humoral immunoreactivity against common allergens in patients formerly classified inside the “intrinsic” asthma endotype.

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“…The Leukocyte Adherence Inhibition Test (LAIT) is an immunoassay used to testify an ex vivo challenge test, monitoring a possible Gell and Coombs type II non-IgEmediated immunoreactivity against diverse types of antigens and allergens [22]- [30].…”

Section: Introductionmentioning

confidence: 99%

Evaluating Non-IgE-mediated Allergens’ Immunoreactivity in Patients with “Intrinsic” Persistent Rhinitis with Help of the Leukocyte Adherence Inhibition Test

Olivier

Pinto

Teixeira

et al. 2023

EJMED

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Background: The non-IgE-mediated immunoreactivities against common allergens are little understood conditions, especially in patients suffering from allergic respiratory disorders such as persistent rhinitis. Objective: The present study aims to evaluate the non-IgE-mediated immunoreactivity against common allergens, in allergen-reactive patients clinically diagnosed with persistent rhinitis without evidence of IgE-mediated hypersensitivity against these allergens. Methods: A group of 533 outpatients diagnosed with non-IgE-mediated allergen-reactive persistent rhinitis, were submitted to ex vivo Leukocyte Adherence Inhibition Tests (LAIT) with extracts of Dermatophagoides pteronyssinus, Hevea brasiliensis latex, dog dander, cat dander, cow’s milk proteins, beekeeping pollen and a mixture of airborne fungal extracts. Results: Cascade distribution column graphs were assembled according to the LAIT results among six ranges of Leukocyte Adherence Inhibition (LAI) of each allergen group. A column graph was plotted with the mean LAI results for each antigen for comparison. Conclusion: The LAIT is a potential tool to quantify the overall non-IgE-mediated cellular/humoral immunoreactivity against common allergens in patients clinically diagnosed with persistent rhinitis. Most studies must be done to evaluate the potential of the LAIT as a screening ex vivo provocation test to select the more reactive allergens to perform the diagnostic in vivo provocation tests.

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The Plasma Preincubation with Papain Before the Assay Suggests that a Gell and Coombs Type II Reaction is Been Demonstrated by the Leukocyte Adherence Inhibition Test

Cited by 4 publications

References 54 publications

Leukocyte Adherence Inhibition Test to the assessment of Immunoreactivity Against Cow’s Milk Proteins in Non—IgE-Mediated Gastrointestinal Food Allergy

Leukocyte Adherence Inhibition Test to the assessment of Immunoreactivity Against Cow’s Milk Proteins in Non—IgE-Mediated Gastrointestinal Food Allergy

Evaluating Non-IgE-Mediated Allergens’ Immunoreactivity in Patients Formerly Classified as “Intrinsic” Asthmatics with Help of the Leukocyte Adherence Inhibition Test

Evaluating Non-IgE-mediated Allergens’ Immunoreactivity in Patients with “Intrinsic” Persistent Rhinitis with Help of the Leukocyte Adherence Inhibition Test

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