The plasma proteome and the acute phase protein response in canine pyometra

Kuleš, Josipa; Horvatić, Anita; Guillemin, Nicolas; Ferreira, Rafaela Furioso; Mischke, R.; Mrljak, Vladimir; Chadwick, Christopher C.; Eckersall, P.D.

doi:10.1016/j.jprot.2020.103817

Cited by 19 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in PON-1 in hypothyroid dogs of our study differs from the decreases described in humans with hypothyroidism; in which low PON-1 values are considered a risk of atherosclerosis associated with this disease [30][31][32][33]. Dogs carry cholesterol in HDL where PON-1 is bounded protecting it from oxidation [34] instead of in LDL, as it occurs in humans. Therefore, the elevation of cholesterol that usually appears in canine hypothyroidism could imply an increase in HDL and subsequently in PON-1.…”

Section: Discussionmentioning

confidence: 53%

Changes in biomarkers of redox status in serum and saliva of dogs with hypothyroidism

et al. 2023

View full text Add to dashboard Cite

Background Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on a dog’s life quality. This study aims to evaluate changes in the redox status in canine hypothyroidism. For this purpose, a comprehensive panel of antioxidants and oxidants biomarkers were measured in serum and saliva of 23 dogs with hypothyroidism, 21 dogs with non-thyroidal illness, and 16 healthy dogs. Among the antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol, paraoxonase type 1 (PON-1) and glutathione peroxidase (GPx) were determined in serum and CUPRAC, ferric reducing ability of saliva (FRAS) and TEAC in saliva. The oxidant biomarkers included were total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP), and thiobarbituric acid reactive substances (TBARS) in serum and AOPP and TBARS in saliva. Results Results showed a significantly higher TEAC, PON-1, GPx, TOS, POX-Act, and d-ROMs, and a significantly lower AOPP in serum of dogs with hypothyroidism. Meanwhile, significantly lower FRAS and AOPP were observed in saliva of dogs with hypothyroidism. Once salivary concentrations were corrected based on their total protein concentrations, the only analyte showing significant changes was TBARS which was significantly higher in dogs with hypothyroidism. Conclusions Our results show that dogs with hypothyroidism present alterations in the redox status in both serum and saliva. This study should be considered a preliminary study and further research addressing these changes should be made using larger populations.

show abstract

Section: Discussionmentioning

confidence: 53%

Changes in biomarkers of redox status in serum and saliva of dogs with hypothyroidism

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The increase in PON-1 in the diseased dogs of our study differs from the decreases described in humans with hypothyroidism; where low PON-1 values are considered a risk of atherosclerosis associated with this disease [30][31][32][33]. Dogs carry cholesterol in HDL where PON-1 is bounded protecting it from oxidation [34] instead of in LDL, as it occurs in humans. Therefore, the elevation of cholesterol that usually appears in canine hypothyroidism could imply an increase in HDL and subsequently in PON-1.…”

Section: Discussionmentioning

confidence: 64%

Changes in biomarkers of redox status in serum and saliva in dogs with hypothyroidism

Arostegui

Muñoz-Prieto

Marín

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on dog’s life quality. This study aims to evaluate changes in the redox status in canine hypothyroidism. For this purpose, a comprehensive panel of antioxidants and oxidants was measured in serum and saliva of 23 dogs with hypothyroidism, 20 dogs with non-thyroidal illness and 16 healthy dogs. Among the antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma or saliva (FRAP or FRAS, respectively), Trolox equivalent antioxidant capacity (TEAC),thiol, paraoxonase type 1 (PON-1) and glutathione peroxidase (GPx) were determined. The oxidant biomarkers included were total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). Results: Results showed a significantly higher TEAC, PON-1, GPx, TOS, POX-Act and d-ROMs, and a significantly lower of AOPP in serum of dogs with hypothyroidism, except when AOPP was corrected by serum albumin concentration. Meanwhile, a significantly lower FRAS and AOPP was observed in saliva of dogs with hypothyroidism. Once salivary concentrations were corrected based on their total protein concentrations, the only analyte showing significant changes was TBARS that was significantly higher in dogs with hypothyroidism Conclusions: Our results show thatdogs with hypothyroidism present alterations in the redox status in both serum and saliva. This study should be considered a preliminary study and further research addressing these changes should be made using larger populations.

show abstract

“…Indeed, it is interesting that the amyloidogenic mutations of such protein have been associated with amyloids deposits (transthyretin amyloidosis), possibly correlated with the progressive damage of the autonomic nervous system, which may be in turn be associated with reduced GI motility [ 9 , 15 ]. Transthyretin is also considered a negative acute phase protein, as it commonly decreases in human neurological disorders, notwithstanding the fact that it was found to be upregulated, in a corticosteroid-related way, in dogs suffering from cervical spondylomyelopathy [ 16 ] but was also found to be decreased in dogs with pyometra [ 17 ]. This protein was also reported in a study conducted in 2017 involving the protein profiles of the fecal material of female cheetahs [ 18 ] and pregnant polar bears [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Mangiaterra

Vincenzetti²,

Rossi³

et al. 2022

Animals

View full text Add to dashboard Cite

Fecal proteomics allows for the identification of proteins and peptides present in stools and is useful in finding possible new biomarkers for diagnosing and/or monitoring gastrointestinal (GI) disorders. In the present study, we investigated the fecal proteome in healthy and diseased cheetahs (Acinonyx jubatus). Captive individuals of this species frequently show gastrointestinal disorders characterized by recurrent episodes of diarrhea, rare episodes of vomiting and weight loss, associated with Helicobacter spp. infection. Fecal proteomic evaluation has been performed by two-dimensional electrophoresis followed by liquid chromatography-tandem mass spectrometry. In healthy cheetahs, the results showed the presence of the following proteins: collagen alpha-1 (II) chain, transthyretin, IgG Fc-binding protein, titin, dystonin, isopentenyl-diphosphate Delta-isomerase 1, sodium/potassium-transporting ATPase subunit alpha-1 and protein disulfide-isomerase A6. The presence of albumin isoforms was found only in diseased cheetahs. The present paper reports the study of the fecal proteome in the cheetah, evidences some differences between healthy and diseased patients and confirms, once again, the potential of fecal proteomics for the study of the GI environment, with promising developments regarding the identification of new diagnostic/monitoring markers.

show abstract

The plasma proteome and the acute phase protein response in canine pyometra

Abstract: The material cannot be used for any other purpose without further permission of the publisher and is for private use only.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.

Cited by 19 publications

References 38 publications

Changes in biomarkers of redox status in serum and saliva of dogs with hypothyroidism

Changes in biomarkers of redox status in serum and saliva of dogs with hypothyroidism

Changes in biomarkers of redox status in serum and saliva in dogs with hypothyroidism

Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Contact Info

Product

Resources

About