The Plasmatic Atmosphere of Blood Platelets. Evidence that only Fibrinogen, AcG, and Activated Hageman Factor are Present on the Surface of Platelets.

Abstract

SummaryWashed platelet suspensions contain active SF (surface factor or activation product), when prepared from normal (N) subjects or from patients deficient in factors Vili, IX or X, but not from a Hageman-deficient. Factor V-deficient plasmas were normalized by platelets from N, XII- and other subjects. Allowing for (a) the platelet prothromboplastic phospholipids, and (b) the above SF and AcG (V) effects, there was no evidence of any significant factors Vili, IX or X in the platelet plasmatic atmosphere. Since XII-platelets showed no true correction of plasmas deficient in Vili, IX or X, the minor “improvement” of test results in these (and N) plasmas on adding N, Vili-, IX- or X-platelets can be explained as a nonspecific surface factor effect. XII-platelets when incubated with normal plasma were normalized by adsorbing Hageman factor (XII) on their surface.

Platelet‐Associated Coagulation Factors: Immunological Detection and the Effect of Calcium

“…Most authors agree that procoagulant factor VIII activity is easily destroyed or removed from platelets by simple washing procedures (Iatridis & Ferguson, 1965;Walsh, 1972a, b, c) and this was confirmed in the present study. In contrast, factor VIII related antigen was shown by immunofluorescence to be persistent in platelet preparations after multiple washes.…”

“…L. Bloom coagulant activities (Walsh,I972a,b) may give rise to difficulties in interpreting conventional coagulation assays in studies of platelets and coagulation factors. In this respect the Surface Factor (SF) activity of platelets described by Iatridis & Ferguson (1965) and the factor XI-like and factor XII-like activity demonstrated by Schiffman et a1 (1973) may be similar to some of the coagulant activities noted by Walsh (1974).…”

“…Roskam (1923) introduced the concept of a 'plasmatic atmosphere' of coagulation factors surrounding platelets and Adelson et a1 (1961) reviewed the evidence supporting the theory that platelets adsorb and concentrate coagulation factors from circulating plasma. More recent reports have indicated that some coagulation factors are more intimately involved with platelets than others (Iatridis & Ferguson, 1965;Walsh, 1972a, b, c) although there appears to be some controversy as to whether factor XI activity remains demonstrable in platelet preparations or whether it is removed by simple washing procedures (Horowitz & Fujimoto, 1965;Walsh, 1972a, b, c;Schiffman et al, 1973). The significance of the relationship between coagulation factors and platelets has been discussed by several authors.…”

Enzymatic Basis for Platelet Aggregation and Release: The Significance of the ‘Platelet Atmosphere’ and the Relationship between Platelet Function and Blood Coagulation

“…The concept of a 'plasmatic atmosphere' of coagulation factors surrounding platelets was originally proposed by Roskam (1923) and this has subsequently been confirmed (Adelson et al, 1961;Iatridis & Ferguson, 1965;Horowitz & Fujimoto, 1965). The significance of this relationship is that blood coagulation and platelet function are interdependent and the two reactions can be regarded as one phenomenon.…”

Influence d’acides gras saturés et non saturés sur la pression de filtration de plasma riche en plaquettes. Correlation avec l’agrégation plaquettaire

“…Si l'intervention directe du facteur Hageman dans l'adhésion des plaquet tes avancée dès 1962 par J ürgens [8] ne semble pas avoir été retenue par les travaux plus récents, et en particulier ceux de Barth et al [1] l'interaction des paquettes et du système contact parait cependant étroite [14]. On peut supposer également que l'activation des facteurs XII et XI, adsorbés au niveau de la membrane plaquettaire [6,7] conduisent à la formation de thrombine dont l'action agrégante pourrait expliquer la différence de comportement des agrégats.…”