2018
DOI: 10.1038/s41598-018-33236-x
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The Plasmodium falciparum male gametocyte protein P230p, a paralog of P230, is vital for ookinete formation and mosquito transmission

Abstract: Two members of 6-cysteine (6-cys) protein family, P48/45 and P230, are important for gamete fertility in rodent and human malaria parasites and are leading transmission blocking vaccine antigens. Rodent and human parasites encode a paralog of P230, called P230p. While P230 is expressed in male and female parasites, P230p is expressed only in male gametocytes and gametes. In rodent malaria parasites this protein is dispensable throughout the complete life-cycle; however, its function in P. falciparum is unknown… Show more

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Cited by 35 publications
(23 citation statements)
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“…Gametocytes of mCherry-luc@gapdh were mCherry positive, again in agreement with our previous observations of the GFP@ gapdh parasites (Marin-Mogollon et al, 2018) (Figure 2B; Supplementary Figure 3B). However, mCherry signals in gametocytes were relatively weak in all stages during their development (stage II-IV).…”
Section: Resultssupporting
confidence: 93%
See 1 more Smart Citation
“…Gametocytes of mCherry-luc@gapdh were mCherry positive, again in agreement with our previous observations of the GFP@ gapdh parasites (Marin-Mogollon et al, 2018) (Figure 2B; Supplementary Figure 3B). However, mCherry signals in gametocytes were relatively weak in all stages during their development (stage II-IV).…”
Section: Resultssupporting
confidence: 93%
“…We selected the P. falciparum p47 gene (PF3D7_1346800) locus for introduction of the transgene expression cassette into the genome as it has been shown to be suitable for transgene expression without compromising parasite development in blood- and liver-stages as well as in A. stephensi mosquitoes (Talman et al, 2010; Vaughan et al, 2012; Vos et al, 2015). We had previously generated various transgenic reporter P. falciparum lines, where GFP-expression cassettes were introduced into the p230p gene locus (PF3D7_0208900), but disruption of this gene resulted in parasites that could not complete mosquito stage development (Marin-Mogollon et al, 2018). Using CRISPR/Cas9 methodology a transgenic P. falciparum (Pf) parasite line was created that encodes an mCherry-luciferase fusion gene under the control of 1.7 kb of 5' UTR of the etramp10.3 gene.…”
Section: Resultsmentioning
confidence: 99%
“…Although the molecular mechanisms of fertilization are poorly understood, this process is a promising target for the interruption of transmission [13]. Several proteins involved in the fertilization process have been identified, including P47, P48/45, P230 and HAP2 [14,15]. HAP2/Generative Cell Specific 1 (GCS1), first identified in the plants Arabidopsis thaliana and Lilium longflorum, is a class II viral fusion protein with a cysteine-rich extracellular region [16][17][18].…”
mentioning
confidence: 99%
“…BamHI and HindIII were used to remove the mevalonate gene from p15-Mev-aSFG and insert the tetR-DOZI coding region, a p2A skip peptide and BSD, as found in pMG74 ( Ganesan et al, 2016 ). We then added homology arms (HA) to facilitate site-specific integration into the p230p gene (PF3D7_0208900), which is dispensable in blood stage parasites ( Marin-Mogollon et al, 2018 ). HA1 and HA2 were sewn together with PCR primers p230HA1.F and p230HA1.NotI.R (for HA1) and primers p230HA2.NotI.F and p230HA2.R (for HA2) and inserted into the NotI site in the backbone of p15-Mev-aSFG ( Supplementary file 1 ).…”
Section: Methodsmentioning
confidence: 99%