The Plasmodium liver-stage parasitophorous vacuole: A front-line of communication between parasite and host

Nyboer, Britta; Heiß, Kirsten; Mueller, Ann‐Kristin; Ingmundson, Alyssa

doi:10.1016/j.ijmm.2017.09.008

Cited by 39 publications

(34 citation statements)

References 120 publications

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“…Only a limited number of PVM proteins have been identified so far, and the functions of most of these remain elusive (reviewed in references 3 and 4 ). These include the earliest known PVM protein, exported protein 1 (EXP1), which contains a classical N-terminal signal peptide and is inserted into the PVM of blood and liver stage parasites with its transmembrane domain, whereby the C terminus faces the host cell cytoplasm ( 5 – 8 ).…”

Section: Introductionmentioning

confidence: 99%

BioID Reveals Novel Proteins of the Plasmodium Parasitophorous Vacuole Membrane

Schnider

Bausch-Fluck

Brühlmann

et al. 2018

mSphere

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Intracellular pathogens are often surrounded by a host-cell derived membrane. This membrane is modified by the pathogens to their own needs and is crucial for their intracellular lifestyle. In Plasmodium parasites, this membrane is referred to as the PVM and only a limited number of its proteins are known so far. Here, we applied in rodent P. berghei parasites a method called BioID, which is based on biotinylation of proximal and interacting proteins by the promiscuous biotin ligase BirA*, and demonstrated its usefulness in identification of novel PVM proteins.

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Section: Introductionmentioning

confidence: 99%

BioID Reveals Novel Proteins of the Plasmodium Parasitophorous Vacuole Membrane

Schnider

Bausch-Fluck

Brühlmann

et al. 2018

mSphere

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“…Development of liver stage parasites occurs within a parasitophorous vacuole (PV), a parasite-derived structure that separates the newly-invaded parasite from the host cell cytoplasm (9). Within this vacuole, the parasite acquires nutrients from its host necessary for growth and replication (8,10,11). Once mature, the PV membrane ruptures, releasing the hepatic merozoites into the host cell cytoplasm (12).…”

Section: Graphical Abstractmentioning

confidence: 99%

Proteomic Analysis ofPlasmodiumMerosomes: The Link Between Liver and Blood Stages in Malaria

Shears

Nirujogi

Swearingen

et al. 2019

Preprint

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The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for anti-malarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We identify a total of 1879 parasite proteins, and a core set of 1188 proteins quantitatively detected in every biological replicate, providing an extensive picture of the protein repertoire of this stage. This unique dataset will allow us to explore key questions about the biology of merosomes and hepatic merozoites.

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“…The Plasmodium parasite, during their development within the hepatocytes, is shielded from the host cytosolic defense mechanisms by the parasite vacuole membrane (PVM). The PVM is originally derived from the host cell plasma membrane, but is extensively modified by the parasite, which inserts its proteins to this membrane ( Meis et al, 1983 ; Lingelbach and Joiner, 1998 ; Nyboer et al, 2017 ). Some of these proteins are therefore likely to directly interact with cytosolic defense mechanisms and subvert them.…”

Section: Autophagy and Plasmodiummentioning

confidence: 99%

“…(A) The sporozoite stage of the Plasmodium parasite invades a hepatocyte within the liver. Following invasion, the parasite resides within a membrane bound parasitophorous vacuole (PV) within the host cell cytosol ( Meis et al, 1983 ; Lingelbach and Joiner, 1998 ; Nyboer et al, 2017 ). The PV membrane (PVM) is recognized immediately after infection by the host.…”

Section: Autophagy and Plasmodiummentioning

confidence: 99%

The Interplay of Host Autophagy and Eukaryotic Pathogens

Evans

Sundaramurthy

Frickel

2018

Front. Cell Dev. Biol.

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For intracellular pathogens, host cells provide a replicative niche, but are also armed with innate defense mechanisms to combat the intruder. Co-evolution of host and pathogens has produced a complex interplay of host-pathogen interactions during infection, with autophagy emerging as a key player in the recent years. Host autophagy as a degradative process is a significant hindrance to intracellular growth of the pathogens, but also can be subverted by the pathogens to provide support such as nutrients. While the role of host cell autophagy in the pathogenesis mechanisms of several bacterial and viral pathogens have been extensively studied, less is known for eukaryotic pathogens. In this review, we focus on the interplay of host autophagy with the eukaryotic pathogens Plasmodium spp, Toxoplasma, Leishmania spp and the fungal pathogens Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans. The differences between these eukaryotic pathogens in terms of the host cell types they infect, infective strategies and the host responses required to defend against them provide an interesting insight into how they respond to and interact with host cell autophagy. Due to the ability to infect multiple host species and cell types during the course of their usually complex lifestyles, autophagy plays divergent roles even for the same pathogen. The scenario is further compounded since many of the eukaryotic pathogens have their own sets of either complete or partial autophagy machinery. Eukaryotic pathogen-autophagy interplay is thus a complex relationship with many novel insights for the basic understanding of autophagy, and potential for clinical relevance.

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The Plasmodium liver-stage parasitophorous vacuole: A front-line of communication between parasite and host

Cited by 39 publications

References 120 publications

BioID Reveals Novel Proteins of the Plasmodium Parasitophorous Vacuole Membrane

BioID Reveals Novel Proteins of the Plasmodium Parasitophorous Vacuole Membrane

Proteomic Analysis ofPlasmodiumMerosomes: The Link Between Liver and Blood Stages in Malaria

The Interplay of Host Autophagy and Eukaryotic Pathogens

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