The Plastidial Glucan Phosphorylase Affects the Maltooligosaccharide Metabolism in Parenchyma Cells of Potato (<i>Solanum tuberosum</i>L.) Tuber Discs

Castellanos, Junio Flores; Fettke, Joerg

doi:10.1093/pcp/pcac174

Cited by 11 publications

(13 citation statements)

References 49 publications

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“…Our results in wheat are in contrast to recent reports that lines deficient in PHS1 have substantial MOS accumulation in rice endosperm and potato tubers ( Dong et al 2023 ; Flores-Castellanos and Fettke 2023 ). In wheat endosperm, loss of PHS1 did not alter MOS levels ( Supplemental Figs.…”

Section: Discussioncontrasting

confidence: 99%

“…Given recent evidence that PHS1 deficiency in potato and rice results in strong MOS accumulation in tubers and endosperm ( Dong et al 2023 ; Flores-Castellanos and Fettke 2023 ), we investigated whether there was MOS accumulation in the developing endosperm of wheat phs1 mutants and quantified the total soluble glucans in perchloric acid extracts. However, levels of MOS, soluble glucans, and other sugars were unaltered in the phs1 mutants.…”

Section: Resultsmentioning

confidence: 99%

“…Overall, these findings suggest PHS1 could play a role in granule initiation, but in the species examined, its importance is conditional on the presence of other mutations or temperature. More recent evidence suggests that PHS1 deficiency in potato ( Solanum tuberosum ) and rice results in strong alterations in MOS levels ( Dong et al 2023 ; Flores-Castellanos and Fettke 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

Kamble,

Makhamadjonov,

Fahy

et al. 2023

The Plant Cell

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The plastidial α-glucan phosphorylase (PHS1) can elongate and degrade maltooligosaccharides (MOSs), but its exact physiological role in plants is poorly understood. Here, we discover a specialized role of PHS1 in establishing the unique bimodal characteristic of starch granules in wheat (Triticum spp.) endosperm. Wheat endosperm contains large A-type granules that initiate at early grain development, and small B-type granules that initiate in later grain development. We demonstrate that PHS1 interacts with B-GRANULE CONTENT1 (BGC1), a carbohydrate-binding protein essential for normal B-type granule initiation. Mutants of tetraploid durum wheat (Triticum turgidum) deficient in all homoeologs of PHS1 had normal A-type granules but fewer and larger B-type granules. Grain size and starch content were not affected by the mutations. Further, by assessing granule numbers during grain development in the phs1 mutant and using a double mutant defective in both PHS1 and BGC1, we demonstrate that PHS1 is exclusively involved in B-type granule initiation. The total starch content and number of starch granules per chloroplast in leaves were not affected by loss of PHS1, suggesting that its role in granule initiation in wheat is limited to the endosperm. We therefore propose that the initiation of A- and B-type granules occurs via distinct biochemical mechanisms, where PHS1 plays an exclusive role in B-type granule initiation.

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Section: Discussioncontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

Kamble,

Makhamadjonov,

Fahy

et al. 2023

The Plant Cell

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show abstract

“…Given recent evidence that PHS1 deficiency in potato and rice results in strong MOS accumulation in tubers and endosperm (Dong et al, 2023;Flores-Castellanos and Fettke, 2023), we investigated whether there was MOS accumulation in the developing endosperm of wheat phs1 mutants. However, levels of MOS, soluble glucans and other sugars were unaltered in the phs1 mutants: We quantified the total soluble glucans in perchloric acid extracts.…”

Section: Loss Of Phs1 In Wheat Does Affect Total Mos Levels In the En...mentioning

confidence: 99%

“…Overall, these findings suggest PHS1 could play a role in granule initiation, but in the species examined, its importance is conditional on the presence of other mutations or temperature. More recent evidence suggests that PHS1 deficiency in potato and rice results in strong alterations in MOS levels (Dong et al, 2023;Flores-Castellanos and Fettke, 2023).…”

Section: Introductionmentioning

confidence: 99%

Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

Kamble,

Makhamadnojov,

Fahy

et al. 2023

Preprint

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PHS1 is a plastidial α-glucan phosphorylase that can elongate and degrade maltooligosaccharides (MOS), but its exact physiological role in plants is poorly understood. Here, we discover a specialised role of PHS1 in establishing the unique bimodal characteristic of starch granules in the wheat endosperm. Wheat endosperm contains large A-type granules that initiate at early grain development, and small B-type granules that initiate in later grain development. We demonstrate that PHS1 interacts with BGC1 - a carbohydrate-binding protein essential for normal B-type granule initiation. Mutants of tetraploid durum wheat deficient in all homeologs of PHS1 had normal A-type granules, but fewer and larger B-type granules. Grain size and starch content were not affected by the mutations. Further, by assessing granule numbers during grain development in the phs1 mutant, and using a double mutant defective in both PHS1 and BGC1, we demonstrate that PHS1 is exclusively involved in B-type granule initiation. The total starch content and number of starch granules per chloroplast in leaves were not affected by loss of PHS1, suggesting that its role in granule initiation in wheat is limited to the endosperm. We therefore propose that the initiation of A- and B-type granules occur via distinct biochemical mechanisms, where PHS1 plays an exclusive role in B-type granule initiation.

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Genome-wide identification of starch phosphorylase gene family in Rosa chinensis and expression in response to abiotic stress

Li,

Chen,

Zhang

et al. 2024

Sci Rep

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Chinese rose (Rosa chinensis) is an important ornamental plant, with economic, cultural, and symbolic significance. During the application of outdoor greening, adverse environments such as high temperature and drought are often encountered, which affect its application scope and ornamental quality. The starch phosphorylase (Pho) gene family participate in the synthesis and decomposition of starch, not only related to plant energy metabolism, but also plays an important role in plant stress resistance. The role of Pho in combating salinity and high temperature stress in R. chinensis remains unknown. In this work, 4 Phos from R. chinensis were detected with Pfam number of Pho (PF00343.23) and predicted by homolog-based prediction (HBP). The Phos are characterized by sequence lengths of 821 to 997 bp, and the proteins are predicted to subcellularly located in the plastid and cytoplasm. The regulatory regions of the Phos contain abundant stress and phytohormone-responsive cis-acting elements. Based on transcriptome analysis, the Phos were found to respond to abiotic stress factors such as drought, salinity, high temperature, and plant phytohormone of jasmonic acid and salicylic acid. The response of Phos to abiotic stress factors such as salinity and high temperature was confirmed by qRT-PCR analysis. To evaluate the genetic characteristics of Phos, a total of 69 Phos from 17 species were analyzed and then classified into 3 groups in phylogenetic tree. The collinearity analysis of Phos in R. chinensis and other species was conducted for the first time. This work provides a view of evolution for the Pho gene family and indicates that Phos play an important role in abiotic stress response of R. chinensis.

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The Plastidial Glucan Phosphorylase Affects the Maltooligosaccharide Metabolism in Parenchyma Cells of Potato (Solanum tuberosumL.) Tuber Discs

Cited by 11 publications

References 49 publications

Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1

Genome-wide identification of starch phosphorylase gene family in Rosa chinensis and expression in response to abiotic stress

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