The Plastome and Chloroplast Biogenesis

Grierson, Donald; Covey, Simon N.

doi:10.1007/978-1-4615-3666-6_3

Cited by 1 publication

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“…Third, mixtures of G. soja and G. max showed that the 250 bp band was synthesized, when mixed at a 1:1 mass ratio suggesting that competition between templates was not a factor. Since, the 250 bp polymorphic band was not synthesized, if G. soja constituted less than 17~o of the mixture (data not shown) and G. soja was used as the male parent, it is likely that the 250 bp AFLP originated from G. soja organelle DNA which constitutes 10 to 20~o of total cellular DNA [12]. Since soybean organelles are presumed to be inherited maternally, the AFLP did not appear in F1 or F2 progeny.…”

Section: Discussionmentioning

confidence: 99%

Inheritance of polymorphic markers generated by DNA amplification fingerprinting and their use as genetic markers in soybean

Prabhu

Gresshoff

1994

Plant Mol Biol

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DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI468.397 and Glycine max (L.) Merr. line nts382, F1 and F2 progeny. The amplification reaction was carried out with soybean genomic DNA and 8 base long oligonucleotide primers. Silver-stained 5% polyacrylamide gels containing 7 M urea detected from 11 to 28 DAF products with primers of varying GC content (ranging from 50 to 100% GC). Depending on their intensity, AFLPs were classified into three classes. DAF profiles were reproducible for different DNA extractions and gels. Forty AFLPs were detected by 26 primers when comparing G. soja and G. max. Most AFLPs were inherited as dominant Mendelian markers in F1 and F2 populations. However, abnormal inheritance occurred with about 25% of polymorphisms. One marker was inherited as a maternal marker, presumably originating from organelle DNA while another showed apparent paternal inheritance. To confirm the nuclear origin and utility of dominant Mendelian markers, three DAF polymorphisms were mapped using a F11 mapping population of recombinant inbred lines from soybean cultivars Minsoy x Noir 1. The study showed that DAF-generated polymorphic markers occur frequently and reliably, that they are inherited as Mendelian dominant loci and that they can be used in genome mapping.

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Section: Discussionmentioning

confidence: 99%