The plastome-encoded zfpA gene of a moss contains procaryotic as well as eucaryotic promoter consensus sequences and its RNA abundance is modulated by cytokinin

Kasten, B.; Wehe, M.; Kruse, Sven; Reutter, K.; Abel, W. O.; Reski, Ralf

doi:10.1007/bf00317930

Cited by 18 publications

(6 citation statements)

References 27 publications

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“…The first mitochondrial DNA sequence from an archegoniate (cox3) was obtained from Physcomitrella , as well as the first chloroplast nucleotide sequences from mosses (Kasten et al, 1992). Subsequently, nucleotide data from this species accumulated (GAPDH: Martin et al, 1993;myb-related genes: Leech et al, 1993;phyB: Kolukisaoglu et al, 1993;rbcS, cab and 25 S rONA: Reski et al, 1994) as well as amino acid data from chloroplast proteins .…”

Section: Finally: Molecular Biology Of Mossesmentioning

confidence: 99%

Development, Genetics and Molecular Biology of Mosses

1998

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The first descriptions of sex chromosomes in plants, of the continuity of chromosomes during the mitotic cycle and of non‐Mendelian inheritance as well as the introduction of UV‐mutagenesis to genetic research are landmarks in biological sciences first achieved by scientists working on bryophytes. Haploidy of the tissue facilitates mutant isolation and many developmental moss mutants have been isolated. Early moss development is triggered by auxin, by cytokinin and by light, mainly acting via phytochrome and a blue‐light receptor. Due to the simplicity of the plants, development can be pinpointed to the differentiation of a single cell and be analysed in living tissue, making mosses ideal candidates for the analysis of development in an integrated approach of cell and molecular biology. Molecular genetic techniques have been applied mainly to Physcomitrella patens (Hedw.) B.S.G., where efficient protocols for transformation of nuclear DNA have been established and several nuclear, chloroplast and mitochondrial genes have been analysed. These studies reveal that Physcomitrella may be an appropriate model to study plant development in molecular terms. Recently, it has been shown that, in this species, nuclear genes can be targeted very efficiently by homologous recombination, now opening the door to reverse genetics for plant biologists.

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Section: Finally: Molecular Biology Of Mossesmentioning

confidence: 99%

Development, Genetics and Molecular Biology of Mosses

1998

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“…, 1990). trnR‐CCG is located between rbcL and accD in the moss Physcomitrella patens (Kasten et al. , 1992; Sugiura et al.…”

Section: Introductionmentioning

confidence: 99%

“…The first nucleotide of the anticodon of tRNA Arg -ACG is modified to an inosine (I), and tRNA Arg -ICG decodes the four CGN codons in the common bean Phaseolus vulgaris (Pfitzinger et al, 1990). trnR-CCG is located between rbcL and accD in the moss Physcomitrella patens (Kasten et al, 1992;Sugiura et al, 2003). trnR-CCG has been considered functional in plastids.…”

Section: Introductionmentioning

confidence: 99%

Plastid transformation reveals that moss tRNA^Arg‐CCG is not essential for plastid function

Sugiura

Sugita²

2004

The Plant Journal

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SummaryThree distinct arginine tRNA genes, trnR-CCG, trnR-ACG, and trnR-UCU, are present in the plastid genome of bryophytes, whereas only the latter two trnR genes are present in the major vascular plants, except for black pine. trnR-CCG is located between rbcL and accD in the moss Physcomitrella patens and it was previously believed to be functional in plastids. However, no trnR-CCG transcript has been detected by Northern hybridization, and the codon usage of CGG is quite low in plastid protein-coding sequences. This raises the possibility that trnR-CCG is non-functional. To investigate this possibility, we integrated a foreign gene into the trnR-CCG coding region via homologous recombination, and constructed stable plastid trnR-CCG knockout moss transformants. The trnR-CCG knock-out transformants grew normally, indicating that the P. patens trnR-CCG gene is not essential for plastid function.

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“…Molecular analysis has also been applied to the organellar genomes of P. patens. Sequences have been determined for the plastid genes, trnR-CGG, encoding for arginine-tRNA (Kasten et al 1992) and zfpA. zfpA is of interest since it shares homology with a putative zinc-finger regulatory gene in Pisum, and furthermore, its expression in P. patens appears to be modulated by cytokinin which is known to induce plastid division (Kasten et al 1992).…”

mentioning

confidence: 99%

“…Sequences have been determined for the plastid genes, trnR-CGG, encoding for arginine-tRNA (Kasten et al 1992) and zfpA. zfpA is of interest since it shares homology with a putative zinc-finger regulatory gene in Pisum, and furthermore, its expression in P. patens appears to be modulated by cytokinin which is known to induce plastid division (Kasten et al 1992). The cytochrome oxidase III gene (cox3) has been isolated and sequenced from the mitochondrion of P. patens (Marienfeld, Reski & Abel 1991).…”

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confidence: 99%

Studying plant development in mosses: the transgenic route

Knight

1994

Plant Cell & Environment

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The current status of transgenic studies in mosses is reviewed with particular attention being given to the mosses Physcomitrella patens and Ceratodon purpureus. This paper reviews the advantages of using mosses as models for higher plants in the study of plant development, and includes developmental processes, already partially characterized at the genetic level by mutant analysis, for which transgenic studies may be applicable. The P. patens transformation process is being studied in this laboratory and details are given for a class of transformants which contain extrachromosomal plasmid DNA. Publications which present the nucleic acid and/or protein sequence for nuclear, chloroplast and mitochondrial genes are reviewed. Areas of research in which transgenic studies promise to complement existing cell biological and physiological approaches are discussed. These include the measurement of calcium levels in mutant and wild‐type transformants expressing the apoaequorin gene and a role for phytochrome gene expression in the establishment of polarity.

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The plastome-encoded zfpA gene of a moss contains procaryotic as well as eucaryotic promoter consensus sequences and its RNA abundance is modulated by cytokinin

Cited by 18 publications

References 27 publications

Development, Genetics and Molecular Biology of Mosses

Development, Genetics and Molecular Biology of Mosses

Plastid transformation reveals that moss tRNA^Arg‐CCG is not essential for plastid function

Studying plant development in mosses: the transgenic route

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The plastome-encoded zfpA gene of a moss contains procaryotic as well as eucaryotic promoter consensus sequences and its RNA abundance is modulated by cytokinin

Cited by 18 publications

References 27 publications

Development, Genetics and Molecular Biology of Mosses

Development, Genetics and Molecular Biology of Mosses

Plastid transformation reveals that moss tRNAArg‐CCG is not essential for plastid function

Studying plant development in mosses: the transgenic route

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Plastid transformation reveals that moss tRNA^Arg‐CCG is not essential for plastid function