The platelet surface glycosylation caused by glycosidase has a strong impact on platelet function

Galectin-1 and platelet factor 4 (CXCL4) induce complementary platelet responses in vitro

“…Desialylation of platelets leads to rapid clearance by the liver through the action of β2-integrins on macrophages and the hepatic Ashwell-Morell receptor [58]. The extent of sialic acid presentation on the platelet surface has been shown to have implications for platelet functions, although it appears somewhat controversial whether platelet activity increases [59] or decreases [30]. Also endogenous neuraminidases have recently been shown to modulate platelet activation [31].…”

Painting proteins to study cardiovascular pathology

“…Another widely used model to study DVT is endothelial damage by the application of 3.5%-10% FeCl 3 to the IVC. This application leads to endothelial injury, resulting in very rapid, platelet-rich thrombus formation, similarly to the arterial thrombosis model [59]. As the two described models are both induced in the IVC, shear and vessel wall are equal, and the thrombi induced can be compared for their cellular components after thrombus formation due to abnormal flow or endothelial injury.…”

“…This may reflect the common observation that cells show an increase release of EV after activation. EVs have shown to be upregulated in patients with endothelial dysfunction or atherosclerosis [42,59], in patients with deep vein thrombosis or pulmonary embolism [60][61][62], in patients with cerebrovascular diseases [63][64][65][66] EV levels were higher in patients that later developed a major adverse cardiovascular and cerebral event [44]. This study also finds that the presence of diabetes and male gender are significantly positively correlated to the number of EVs, a factor that has to be taken into account during risk stratification.…”

“…Desialylation of platelets leads to rapid clearance by the liver through the action of β2-integrins on macrophages and the hepatic Ashwell-Morell receptor [58]. The extent of sialic acid presentation on the platelet surface has been shown to have implications for platelet functions, although it appears somewhat controversial whether platelet activity increases [59] or decreases [30]. Also endogenous neuraminidases have recently been shown to modulate platelet activation [31].…”

“…Many small peptides and DTPA chelators are used in the clinic without any toxicity, but there are many differences between mice and humans. Due to their small size, shear stress is smaller in rodents [59]. However, mice are not just small men.…”