The polar lipid composition of walsby's square bacterium

Oren, A

doi:10.1016/0378-1097(96)00085-7

Cited by 46 publications

(59 citation statements)

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“…Cellular fatty acids of photoheterotrophically grown cultures were analysed as described by Sasser (1990). Polar lipid analyses were performed using 1 g freeze-dried cells of photoheterotrophically grown cultures as described previously (Subhash et al, 2013b, c;Oren et al, 1996). Quinone analysis was performed after extraction with chloroform and methanol (2 : 1, v/v) by previously described methods (Imhoff, 1984;.…”

Section: Rhodobacter Ovatus Ja234 T (Am690348)mentioning

confidence: 99%

Rhodobacter sediminis sp. nov., isolated from lagoon sediments

Yadav

Lee

2016

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, rod-shaped phototrophic bacteria (designated strains N1 T and C7) were isolated from lagoon sediments. Both strains were positive for catalase and oxidase activity. Casein, starch, urea and Tween 20 were hydrolysed by both strains while chitin, gelatin and Tween 80 were not. In both strains, C 16 : 0 , C 18 : 0 ,C 16 : 1 !6c/C 16 : 1 !7c and C 18 : 1 !6c/ C 18 : 1 !7c were the predominant fatty acids, with minor amounts of C 8 : 0 3-OH, anteiso-C 14 : 0 , C 17 : 0 , C 14 : 1 !5c, C 17 : 1 10-methyl and C 18 : 1 !5c. Strains N1 T and C7 contained phosphatidylglycerol and phosphatidylethanolamine as major polar lipids with minor amounts of phosphatidylcholine, unidentified lipids and an unidentified phospholipid. The mean genomic DNA G+C content was 70.6±1 mol% and the two strains were closely related (mean DNA-DNA hybridization >90 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains clustered with species of the genus Rhodobacter belonging to the family Rhodobacteraceae of the class Alphaproteobacteria. Strain N1 T has a 16S rRNA gene sequence similarity of 99.2 % with Rhodobacter capsulatus ATCC 11166 T , 99.1 % with Rhodobacter viridis JA737 T and <96.6 % with other members of the genus Rhodobacter. Strain N1 T and C7 shared 100 % 16S rRNA gene sequence similarity. DNA-DNA hybridization values between strain N1 T and the type strains of the nearest species were clearly below the 70 % threshold. On the basis of phenotypic and genotypic data, it is proposed that strain N1 T represents a novel species of the genus Rhodobacter, for which the name Rhodobacter sediminis sp. nov. is proposed. The type strain is N1 T (=KEMB 563-471 T =JCM 31175 T ), and strain C7 is an additional strain of the species.

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Section: Rhodobacter Ovatus Ja234 T (Am690348)mentioning

confidence: 99%

Rhodobacter sediminis sp. nov., isolated from lagoon sediments

Yadav

Lee

2016

International Journal of Systematic and Evolutionary Microbiology

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“…However, the dominant type of halophilic Archaea inhabiting such ponds still defies microbiologists ' attempts toward its isolation (Benlloch et al, 1995 ;Oren et al, 1996).…”

Section: Gibbonsii Haloferax Denitrificans Haloarcula Hispanica Hamentioning

confidence: 99%

“…Polar lipid extraction and characterization by TLC on silica gel plates (20i20 cm ; Sigma) was performed as described by Torreblanca et al (1986), using single development in chloroform\methanol\acetic acid\water (85 : 22n5:10:4, v\v). Plates were stained for glycolipids and other lipids using the α-naphthol stain, the CeSO % \H # SO % stain, the orcinol\FeCl $ stain and the molybdate stain for detection of phospholipids (Kates, 1972 ;Oren et al, 1996 ;Torreblanca et al, 1986). The presence of C #!…”

Section: Collection Of Samples and Isolation Of Halophilic Archaeamentioning

confidence: 99%

Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Montalvo‐Rodríguez¹,

López‐Garriga²,

Vreeland³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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An extremely halophilic Archaeon belonging to the order Halobacteriales was isolated from the solar salterns of Cabo Rojo, Puerto Rico. The organism, designated strain PR5 T , is rod-shaped, non-motile and requires at least 12 % (w/v) NaCl to grow. The strain is highly thermotolerant : its temperature optimum is 50 SC and growth is possible up to 60 SC. Polar lipid analysis revealed the presence of the bis-sulfated glycolipid S 2 -DGD-1 as sole glycolipid and the absence of the glycerol diether analogue of phosphatidylglycerosulfate. Both C 20 ,C 20 and C 20 ,C 25 core lipids are present. The GMC content of the DNA is 63 3 mol %. According to 16S rDNA sequence data, strain PR5 T is closely related to the representatives of the genera Haloterrigena and Natrinema, but on the basis of its phenotypic properties, 16S rDNA sequence and DNA-DNA hybridization studies, strain PR5 T cannot be assigned to any of the recognized species within these genera. On the basis of its polar lipid composition, the isolate has been assigned to the genus Haloterrigena. The creation of a new species, Haloterrigena thermotolerans, is therefore proposed to accommodate this isolate. The type strain is strain PR5 T (l DSM 11552 T l ATCC 700275 T ).

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“…in the first dimension and chloroform\methanol\acetic acid\water (80 : 12 : 15 : 4 by vol.) in the second dimension (Tindall et al, 1987 ;Oren et al, 1996). Phospholipids were visualized with an ammonium molybdate\sulfuric acid spray.…”

Section: Methodsmentioning

confidence: 99%

Halorubrum tebenquichense sp. nov., a novel halophilic archaeon isolated from the Atacama Saltern, Chile.

Lizama¹,

Monteoliva‐Sánchez²,

Suárez-García³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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A novel extremely halophilic archaeon was isolated from Lake Tebenquiche, situated in the northern part of the Atacama Saltern, Chile. The cells of these micro-organisms were mostly irregularly disc-shaped. They grew in medium containing saturated concentrations of NaCl and did not require magnesium for optimal growth. The polar lipid composition revealed the presence of mannosyl-2-sulfate-(1-4)-glycosyl-archaeol, the main glycolipid of the genus Halorubrum, and two new glycolipids. The GMC content of the DNA was 63 2 mol %. Phylogenetic analysis of the 16S rRNA gene placed strain ALT6-92 T within the Halorubrum cluster. The low DNA-DNA hybridization value justified classification in a new species for which the name Halorubrum tebenquichense sp. nov. is proposed. The type strain is ALT6-92 T (l CECT 5317 T l DSM 14210 T ).

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The polar lipid composition of walsby's square bacterium

Cited by 46 publications

References 1 publication

Rhodobacter sediminis sp. nov., isolated from lagoon sediments

Rhodobacter sediminis sp. nov., isolated from lagoon sediments

Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Halorubrum tebenquichense sp. nov., a novel halophilic archaeon isolated from the Atacama Saltern, Chile.

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