The Poly(C) Motif in the Proximal Promoter Region of the D Site-Binding Protein Gene (<i>Dbp</i>) Drives Its High-Amplitude Oscillation

Kwon, Paul Kwangho; Kim, Hyo-Min; Kim, Sung Wook; Kang, Byung Ha; Yi, Hee; Ku, Hyun-Ok; Roh, Tae-Young

doi:10.1128/mcb.00101-19

Cited by 7 publications

(35 citation statements)

References 29 publications

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“…We previously reported that the poly(C) motif of Dbp supports the Dbp mRNA oscillation. 28 We also demonstrated that hnRNP K could act as a trans-acting factor of the poly(C) motif within the Dbp proximal promoter. 7 This led us to wonder whether there was any other poly(C) motif within the proximal promoter regions (promoter region of 1 kb from the TSS) of other clock genes.…”

Section: Rorγ Is a Clock Gene That Contains The Poly(c) Motif A Pomentioning

confidence: 63%

“…The extraction of total RNA was performed as previously described 28 . We used real‐time PCR (Applied Biosystems) with SYBR Green Master (Roche) to measure endogenous mRNA levels.…”

Section: Methodsmentioning

confidence: 99%

“…Treatment with glycine (0.125 M) was performed for 10 minutes to stop the reaction of fixation following the treatment of 1% formaldehyde in NIH3T3 cells. Chromatin immunoprecipitation (ChIP) assays were performed as previously described 7,28 . Anti‐hnRNP K antibody (Abcam, ab70492) and anti‐RNAPII (Abcam, 8WG16) were added to the lysate to immunoprecipitate hnRNP K and RNAPII.…”

Section: Methodsmentioning

confidence: 99%

“…The knockdown of hnRNP K decreased the chromatin accessibility levels of RORγ1 and RORγ2. Dbp exon 4 region was previously used as a negative control 28 (n = 3, n.s. : not significant, indicated by asterisks; *P < .05 by two-way ANOVA followed by Sidak's multiple comparisons test, error bar: SEM) of the aforementioned metabolic genes that showed measurable mRNA expression levels in NIH3T3 cells and quantified their mRNA oscillation levels under the knockdown of hnRNP K (Figure 3).…”

Section: Hnrnp K Supports Mrna Oscillation Of Metabolic Genes Via Rorγmentioning

confidence: 99%

“…Previously, we reported that the poly(C) motif in the promoter region modulates the binding of RNA polymerase 2 (RNAPII) and chromatin accessibility, which affects downstream target gene expression 28 . Moreover, we reported that hnRNP K interacts with the poly(C) motif 7 .…”

Section: Introductionmentioning

confidence: 99%

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hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling

et al. 2021

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Most living organisms on Earth have a circadian rhythm, which gives them the ability to adapt to daily changes. The circadian rhythm regulates gene expression, metabolism, and physiology to sustain biological systems. 1,2 The expressions of genes that participate in the circadian rhythm, which are called clock genes, are delicately regulated by multiple processes such as transcriptional regulation and post-transcriptional regulation. 3-5 Recently, it was reported that heterogeneous nuclear ribonucleoprotein K (hnRNP K) controls the expressions of clock genes such as Dbp and Per3. 6,7 Here, we investigate transcriptional activation of RORγ by hnRNP K, which interacts with the poly(C) motif of RORγ. hnRNP K is known as a durable poly(C)-binding protein 7-11 that controls multiple gene expression processes such

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Section: Rorγ Is a Clock Gene That Contains The Poly(c) Motif A Pomentioning

confidence: 63%

“…The extraction of total RNA was performed as previously described 28 . We used real‐time PCR (Applied Biosystems) with SYBR Green Master (Roche) to measure endogenous mRNA levels.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Hnrnp K Supports Mrna Oscillation Of Metabolic Genes Via Rorγmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling

et al. 2021

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Inhibition of DDX3 and COX-2 by forskolin and evaluation of anti-proliferative, pro-apoptotic effects on cervical cancer cells: molecular modelling and in vitro approaches

et al. 2022

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Cell-type specific circadian bioluminescence rhythms inDbpreporter mice

Smith

Vinne

McCartney

et al. 2021

Preprint

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Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (Dbp). In this line of mice, firefly luciferase is expressed from the Dbp locus in a Cre-recombinase-dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was Cre-independent was also generated. The Dbp reporter alleles do not alter Dbp gene expression rhythms in liver or circadian locomotor activity rhythms. In vitro and In vivo studies show the utility of the reporter alleles for monitoring rhythmicity. Our studies reveal cell-type specific characteristics of rhythms among neuronal populations within the suprachiasmatic nuclei in vitro. In vivo studies show stable Dbp-driven bioluminescence rhythms in the liver of Albumin-Cre;DbpKI/+ liver reporter mice. After a shift of the lighting schedule, locomotor activity achieved the proper phase relationship with the new lighting cycle more rapidly than hepatic bioluminescence did. As previously shown, restricting food access to the daytime altered the phase of hepatic rhythmicity. Our model allowed assessment of the rate of recovery from misalignment once animals were provided with food ad libitum. These studies provide clear evidence for circadian misalignment following environmental perturbations and reveal the utility of this model for minimally invasive, longitudinal monitoring of rhythmicity from specific mouse tissues.

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The Poly(C) Motif in the Proximal Promoter Region of the D Site-Binding Protein Gene (Dbp) Drives Its High-Amplitude Oscillation

Cited by 7 publications

References 29 publications

hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling

hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling

Inhibition of DDX3 and COX-2 by forskolin and evaluation of anti-proliferative, pro-apoptotic effects on cervical cancer cells: molecular modelling and in vitro approaches

Cell-type specific circadian bioluminescence rhythms inDbpreporter mice

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