The polycotyledon (pct1-2) mutant of tomato shows enhanced accumulation of PIN1 auxin transport facilitator protein

Kharshiing, Eros; Kumar, Gaurav; Ditengou, Franck Anicet; Li, X.; Palme, Klaus; Sharma, Rameshwar

doi:10.1111/j.1438-8677.2009.00267.x

Cited by 14 publications

(20 citation statements)

References 29 publications

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“…The pct1-2 mutant of tomato displays nearly 3-fold faster polar auxin transport that is associated with increased expression of the PIN1 auxin transporter in roots (Al-Hammadi et al, 2003;Kharshiing et al, 2010). Consistent with our hypothesis, time-lapse imaging of root penetration in pct1-2 demonstrated that, in contrast to the wild type, pct1-2 roots had nearly normal penetration in the Soilrite in the presence of 1-MCP, with only occasional seedlings showing an aerial root ( Fig.…”

Section: An Auxin Transport Mutant Displays Normal Root Penetration Imentioning

confidence: 99%

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

et al. 2011

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During seed germination, emerging roots display positive gravitropism and penetrate into the soil for nutrition and anchorage. Tomato (Solanum lycopersicum) seeds germinated in the presence of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, failed to insert roots into Soilrite and grew in the air, forming loops. Time-lapse video imaging showed that 1-MCPgrown root tips retained positive gravitropism and made contact with the surface of Soilrite but failed to penetrate into the Soilrite. Time-course studies revealed that the effect of 1-MCP was most prominent when seed imbibition and germination were carried out in the continual presence of 1-MCP. Conversely, 1-MCP was ineffective when applied postgermination after penetration of roots in the Soilrite. Furthermore, treatment with 1-MCP caused a reduction in DR5::b-glucuronidase auxinreporter activity and modified the expression of SlIAA3 and SlIAA9 transcripts, indicating interference with auxin signaling. The reduced ethylene perception mutant, Never-ripe, displayed decreased ability for root penetration, and the enhanced polar auxin transport mutant, polycotyledon, showed a nearly normal root penetration in the presence of 1-MCP, which could be reversed by application of auxin transport inhibitors. Our results indicate that during tomato seed germination, a coaction between ethylene and auxin is required for root penetration into the soil.

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Section: An Auxin Transport Mutant Displays Normal Root Penetration Imentioning

confidence: 99%

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

et al. 2011

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“…The initiation of shoot primordia involves YUCCA regulated auxin biosynthesis and transport facilitated by PIN1 transporter [57]. In conformity with above, IAA levels were higher in RS of pct1-2 mutant that over-accumulates PIN1 protein [27]. The reduction in IAA levels in RS of shr mutant is likely related to the multifaceted interactions of NO with plant hormones [31].…”

Section: Discussionmentioning

confidence: 81%

“…Consistent with this shoot regeneration in Arabidopsis pin1-4 mutant is reduced to 1/5 of wild-type [14]. In view of above role of PIN1 in calli formation, we compared metabolic shifts with the polycotyledon ( pct1-2 ) mutant of tomato that shows enhanced polar auxin transport associated with elevated level of polar auxin transporter PIN1 protein [26], [27]. …”

Section: Introductionmentioning

confidence: 93%

Metabolomic homeostasis shifts after callus formation and shoot regeneration in tomato

et al. 2017

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Plants can regenerate from a variety of tissues on culturing in appropriate media. However, the metabolic shifts involved in callus formation and shoot regeneration are largely unknown. The metabolic profiles of callus generated from tomato (Solanum lycopersicum) cotyledons and that of shoot regenerated from callus were compared with the pct1-2 mutant that exhibits enhanced polar auxin transport and the shr mutant that exhibits elevated nitric oxide levels. The transformation from cotyledon to callus involved a major shift in metabolite profiles with denser metabolic networks in the callus. In contrast, the transformation from callus to shoot involved minor changes in the networks. The metabolic networks in pct1-2 and shr mutants were distinct from wild type and were rewired with shifts in endogenous hormones and metabolite interactions. The callus formation was accompanied by a reduction in the levels of metabolites involved in cell wall lignification and cellular immunity. On the contrary, the levels of monoamines were upregulated in the callus and regenerated shoot. The callus formation and shoot regeneration were accompanied by an increase in salicylic acid in wild type and mutants. The transformation to the callus and also to the shoot downregulated LST8 and upregulated TOR transcript levels indicating a putative linkage between metabolic shift and TOR signalling pathway. The network analysis indicates that shift in metabolite profiles during callus formation and shoot regeneration is governed by a complex interaction between metabolites and endogenous hormones.

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“…Downregulation of the GA biosynthesis gene GA20OX showed delayed flowering in FR enriched environment suggesting a role for this gene in phytochrome-dependent flowering (Hisamatsu and King, 2008). While the association of light and auxin signaling is well established, the tomato pct1-2 mutant having enhanced polar transport of auxin shows delayed phototropism (Kharshiing et al, 2010a,b) as well as increased number of flowers which bloom at the same time as compared to wild type which have lesser flowers and which bloom sequentially (Al-Hammadi et al, 2003). However, the flowers of the pct1-2 mutant are male sterile because the anthers lack dehiscence.…”

Section: Photoreceptors and Phytohormones: Light Mediated Hormonal Rementioning

confidence: 99%

Photoreceptor Mediated Plant Growth Responses: Implications for Photoreceptor Engineering toward Improved Performance in Crops

Oil¹,

Kharshiing²

2017

Front. Plant Sci.

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Rising temperatures during growing seasons coupled with altered precipitation rates presents a challenging task of improving crop productivity for overcoming such altered weather patterns and cater to a growing population. Light is a critical environmental factor that exerts a powerful influence on plant growth and development ranging from seed germination to flowering and fruiting. Higher plants utilize a suite of complex photoreceptor proteins to perceive surrounding red/far-red (phytochromes), blue/UV-A (cryptochromes, phototropins, ZTL/FKF1/LKP2), and UV-B light (UVR8). While genomic studies have also shown that light induces extensive reprogramming of gene expression patterns in plants, molecular genetic studies have shown that manipulation of one or more photoreceptors can result in modification of agronomically beneficial traits. Such information can assist researchers to engineer photoreceptors via genome editing technologies to alter expression or even sensitivity thresholds of native photoreceptors for targeting aspects of plant growth that can confer superior agronomic value to the engineered crops. Here we summarize the agronomically important plant growth processes influenced by photoreceptors in crop species, alongwith the functional interactions between different photoreceptors and phytohormones in regulating these responses. We also discuss the potential utility of synthetic biology approaches in photobiology for improving agronomically beneficial traits of crop plants by engineering designer photoreceptors.

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The polycotyledon (pct1-2) mutant of tomato shows enhanced accumulation of PIN1 auxin transport facilitator protein

Cited by 14 publications

References 29 publications

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

Metabolomic homeostasis shifts after callus formation and shoot regeneration in tomato

Photoreceptor Mediated Plant Growth Responses: Implications for Photoreceptor Engineering toward Improved Performance in Crops

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