The polymerase chain reaction: an epidemiological tool to differentiate between two clusters of pathogenic Yersinia enterocolitica strains

Ibrahim, Ashraf S.; Liesack, Werner; Pike, Stephen; Stackebrandt, Erko

doi:10.1016/0378-1097(92)90364-t

Cited by 8 publications

(6 citation statements)

References 6 publications

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“…The average corrected sequence dissimilarity value between strains of these two subgroups is 1.2%, but is less than 0.5% within each of the subgroups. The phylogenetic splitting of this species is supported by several reports in the literature which indicate both genotypic and phenotypic diversity exists among Y. enterocolitica strains [23][24][25]. Studies have been initiated to confirm the phylogenetic results using both DNA-DNA similarity studies and other genotypic and phenotypic markers (Ibrahim and co-workers, in preparation).…”

Section: Resultsmentioning

confidence: 75%

The phylogeny of the genusYersiniabased on 16S rDNA sequences

Ibrahim

Goebel

Liesack

et al. 1993

FEMS Microbiology Letters

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The inter- and intrageneric relationships of the genus Yersinia were investigated by sequence analysis of the 16S rRNA gene. A stretch of approximately 1450 nucleotides was sequenced from representatives of ten of the eleven validly described species. Phylogenetic analysis revealed that yersinae form a coherent cluster within the gamma subgroup of Proteobacteria. The intrageneric relationship was characterized by five sublines with Y. enterocolitica, Y. rohdei, and Y. ruckeri forming separate sublines each represented by a single species. A separate subline was formed by Y. pestis, Y pseudotuberculosis and Y. kristensenii, while Y. mollaretii, Y. intermedia, Y. bercovieri, Y. aldovae, and Y. kristensenii formed a fifth subline. The phylogenetic distinctness of the yersiniae sublines is compared to published phenotypic properties and results of DNA-DNA similarity studies.

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Section: Resultsmentioning

confidence: 75%

The phylogeny of the genusYersiniabased on 16S rDNA sequences

Ibrahim

Goebel

Liesack

et al. 1993

FEMS Microbiology Letters

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“…In addition to the evaluation of the multiplex PCR assay performed on a number of strains from our culture collection (Tables 1 and 2) and in contrast to the approach of Weynants et al (35), we tested the multiplex PCR on isolates (uncharacterized) obtained in the present study from yersiniosis patients and pork meat (Tables 4 and 5). Several investigators have described multiplex PCR assays for Y. enterocolitica, some for detection (applied to enrichment media) (3,25) and others for identification (applied to pure cultures) (20,22). When the former strategy is used, however, problems with PCR inhibitors are often encountered, and an additional sample preparation step prior to the PCR is required (30).…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of Pathogenic Yersinia enterocolitica Isolates by PCR and Pulsed-Field Gel Electrophoresis

Lambertz

Danielsson-Tham

2005

Appl Environ Microbiol

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Approximately 550 to 600 yersiniosis patients are reported annually in Sweden. Although pigs are thought to be the main reservoir of food-borne pathogenic Yersinia enterocolitica, the role of pork meat as a vehicle for transmission to humans is still unclear. Pork meat collected from refrigerators and local shops frequented by yersiniosis patients (n ‫؍‬ 48) were examined for the presence of pathogenic Yersinia spp. A combined culture and PCR method was used for detection, and a multiplex PCR was developed and evaluated as a tool for efficient identification of pathogenic food and patient isolates. The results obtained with the multiplex PCR were compared to phenotypic test results and confirmed by pulsed-field gel electrophoresis (PFGE). In all, 118 pork products (91 raw and 27 ready-to-eat) were collected. Pathogenic Yersinia spp. were detected by PCR in 10% (9 of 91) of the raw pork samples (loin of pork, fillet of pork, pork chop, ham, and minced meat) but in none of the ready-to-eat products. Isolates of Y. enterocolitica bioserotype 4/O:3 were recovered from six of the PCR-positive raw pork samples; all harbored the virulence plasmid. All isolates were recovered from food collected in shops and, thus, none were from the patients' home. When subjected to PFGE, the six isolates displayed four different NotI profiles. The same four NotI profiles were also present among isolates recovered from the yersiniosis patients. The application of a multiplex PCR was shown to be an efficient tool for identification of pathogenic Y. enterocolitica isolates in naturally contaminated raw pork.

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“…The application of MLEE to study genetic heterogeneity of Y. enterocolitica-like species has also led to important observations. A single strain, each of Y. intermedia, Y. frederiksenii, Y. mollaretii and Y. kristensenii [48][49][50][51][52][53][54][55] gave distinctive electrophoretic type. These electrophoretic types were quite different from those of Y. enterocolitica [7].…”

Section: Multilocus Enzyme Electrophoresismentioning

confidence: 99%

Molecular heterogeneity inYersinia enterocoliticaand âY. enterocolitica-likeâ species â Implications for epidemiology, typing and taxonomy

Virdi

Sachdeva

2005

FEMS Immunology &Amp; Medical Microbiology

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Yersinia enterocolitica is an extremely heterogeneous species. Serotyping and biotyping have been used extensively, in the past, to study its heterogeneity and epidemiology. Application of methods like ribotyping, pulsed-field gel electrophoresis and a host of other genomic techniques have further revealed molecular heterogeneity in this species. Furthermore, these methods may be used effectively to supplement serotyping and biotyping schema for studying epidemiology of Y. enterocolitica. This is evident from the ability of some of these methods to subtype strains belonging to serogroups O:3, O:9 and O:8 - which are most commonly encountered in human Yersiniosis. Multilocus enzyme electrophoresis and nucleotide sequencing have reiterated the taxonomic relationships of this organism. However there is paucity of information about the molecular heterogeneity of 'Y. enterocolitica-like' species, which need to be addressed in the future. Also, newer techniques such as amplified fragment length polymorphism, VNTR-based typing and multilocus sequence typing should be applied to further understand epidemiology, population structure and evolutionary genetics of Y. enterocolitica and 'Y. enterocolitica-like' species.

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The polymerase chain reaction: an epidemiological tool to differentiate between two clusters of pathogenic Yersinia enterocolitica strains

Cited by 8 publications

References 6 publications

The phylogeny of the genusYersiniabased on 16S rDNA sequences

The phylogeny of the genusYersiniabased on 16S rDNA sequences

Identification and Characterization of Pathogenic Yersinia enterocolitica Isolates by PCR and Pulsed-Field Gel Electrophoresis

Molecular heterogeneity inYersinia enterocoliticaand âY. enterocolitica-likeâ species â Implications for epidemiology, typing and taxonomy

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The polymerase chain reaction: an epidemiological tool to differentiate between two clusters of pathogenic Yersinia enterocolitica strains

Cited by 8 publications

References 6 publications

The phylogeny of the genusYersiniabased on 16S rDNA sequences

The phylogeny of the genusYersiniabased on 16S rDNA sequences

Identification and Characterization of Pathogenic Yersinia enterocolitica Isolates by PCR and Pulsed-Field Gel Electrophoresis

Molecular heterogeneity inYersinia enterocoliticaand âY. enterocolitica-likeâ species â Implications for epidemiology, typing and taxonomy

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Molecular heterogeneity inYersinia enterocoliticaand âY. enterocolitica-likeâ species â Implications for epidemiology, typing and taxonomy