The polymerase chain reaction in the demonstration of monoclonality in T cell lymphomas.

Diss, TC; Watts, Mike; Pan, Langxing; Burke, Margaret; Linch, David C.; Isaacson, Peter G.

doi:10.1136/jcp.48.11.1045

Cited by 143 publications

(105 citation statements)

References 26 publications

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“…However, TcR-␥ gene rearrangement is not restricted to T-cell lineage and can occur in various B-cell lymphomas, although rarely in mature neoplastic B-cell proliferations (42)(43)(44). Of note is the detection of a clonal IgH gene rearrangement in approximately 10% of T-cell lymphomas (45)(46)(47), which is of the same degree of magnitude as the detection of TcR-␥ rearrangement in B-cell lymphomas (approximately 5% of cases; 2, 47, 48). It is noteworthy that the presence of clonal TcR-␥ gene rearrangement was mainly found in B-cell lymphoma subtypes, such as follicular, MALT-type, and T-cell-rich B-cell lymphomas, which are known to contain large numbers of T lymphocytes admixed with neoplastic B cells.…”

Section: Discussionmentioning

confidence: 99%

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

et al. 2000

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This report summarizes a cumulative 4-year experience in polymerase chain reaction (PCR) analysis of immunoglobin heavy chain (IgH) and TcR-␥ chain gene rearrangements in 525 cases of lymphoproliferative disorders. Because the sensitivity of the PCR methodology was found to be tissue dependent, in the study of the presence of clonal cell population in tissues containing a small number of polyclonal lymphocytes, such as skin and gastrointestinal biopsy specimens, we used the multiple-PCR run approach. In this latter methodology, we repeat the PCR reaction from the same sample at least three times to confirm the reproducibility of the results. In the study of 273 cases of B-or T-cell lymphomas with characteristic immunomorphological and clinical features, a clonal IgH or TcR-␥ chain gene rearrangement was detected in approximately 80% of cases. A clonal rearrangement involving both IgH and TcR-␥ chain genes was found in 10% of cases of both B-cell and T-cell lymphomas. The study of 167 cases of nonneoplastic lymphoid tissue samples showed the presence of clonally rearranged cell populations for IgH or TcR-␥ genes in 3 and 9% of cases, respectively. We also applied PCR for the study of 85 cases of lymphoproliferations with no definite diagnosis (i.e., benign versus malignant) after immunomorphological analysis. In 65 cases (76%), the correlation of immunomorphological features with the presence (48 cases) or the absence (17 cases) of clonal lymphoid cell populations led to a definite diagnosis. In almost all these cases, the final diagnosis was found to be in agreement with the clinical course. In the 20 remaining cases (24%), no definite diagnosis could be made. We also assessed the value of PCR in detecting bcl-2/J H gene rearrangement as an additional clonal marker in the diagnosis of follicular lymphoma. Bcl-2/J H rearrangement and/or IgH gene rearrangement was found in approximately 85% (71/85) of follicular lymphoma cases studied.

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Section: Discussionmentioning

confidence: 99%

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

et al. 2000

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“…These tests were done with known positive and negative controls. 7 In one case PCR was unsuccessful as significant amount of DNA could be extracted.…”

Section: Cytologymentioning

confidence: 99%

Cytopathological analysis of vitreous in intraocular lymphoma

Intzedy

Teoh

Hogan

et al. 2007

Eye

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Objective To describe the cytopathological method used in the analysis of vitreous samples in the diagnosis of primary intraocular lymphoma (PIOL). Participants Seven patients with refractory posterior uveitis referred to a regional ocular inflammatory service were diagnosed as having PIOL between 1999 and 2006. Methods Clinical features of the uveitis and cytopathological preparation of the samples were described. All patients underwent vitrectomy and samples were placed in formal saline or prepared fresh. Following paraffin embedding generating a cell block, immunostaining, and polymerase chain reactions were performed. Results Five women (71.4%) and two men (28.6%) (mean age 67.7 years) were included. Five patients had diagnostic vitrectomy performed within 6 months of presentation, but in two patients diagnosis was delayed up to 2 years. Uveitis was bilateral in two patients. Cytologic and immunohistochemical staining prepared from the vitreous specimens showed PIOL in all patients, and PCR displayed single band of immunoglobulin heavy chain rearrangement in five out of six samples tested. Conclusions Diagnosis of PIOL is difficult due to small volume of sample with low number of malignant cells and inadequate preparation of samples. Our method of analysis with fresh samples together with immunohistochemistry and PCR analysis demonstrates a high yield of diagnosis reducing diagnostic delay.

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“…The TcR-␥-chain gene was examined by PCR as described by Diss et al (1995). Briefly, three sets of primers, VrIϩVrIII/ivϩJr1/2 (product size approximately 70 to 95 base pairs), VrIϩVrIII/ivϩJpr1/2 (product size approximately 80 to 110 base pairs), and VrIIϩJr1/2 (product size approximately 150 to 180 base pairs) were used separately in total volumes of 40 l of reaction mixture, which contained 10 mM Tris-HCl (pH 8.8), 1.5 mM MgCl 2 , 50 mM KCl, 0.1% Triton X-100, 200 M of each dNTP, 1 mM of each primer, 100 ng of DNA, and 1 unit of Taq polymerase.…”

Section: T-cell Receptor ␥ Rearrangement Studymentioning

confidence: 99%

Expression of Cyclin-Dependent Kinase 6 (cdk6) and Frequent Loss of CD44 in Nasal-Nasopharyngeal NK/T-Cell Lymphomas: Comparison with CD56-Negative Peripheral T-Cell Lymphomas

Lien

Lin

Huang

et al. 2000

Laboratory Investigation

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The polymerase chain reaction in the demonstration of monoclonality in T cell lymphomas.

Abstract: Aims-To evaluate polymerase chain reaction (PCR) amplification of T cell receptor (TCR) P and y chain genes as a means of demonstrating monoclonality in T cell lymphomas using histological samples; to compare the performance of PCR with Southern blot analysis.

Cited by 143 publications

References 26 publications

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

Cytopathological analysis of vitreous in intraocular lymphoma

Expression of Cyclin-Dependent Kinase 6 (cdk6) and Frequent Loss of CD44 in Nasal-Nasopharyngeal NK/T-Cell Lymphomas: Comparison with CD56-Negative Peripheral T-Cell Lymphomas

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