The polymeric antigen BLSOmp31 confers protection against Brucella ovis infection in rams

Estein, Silvia Marcela; Fiorentino, Marí­a Andrea; Paolicchi, Fernando Alberto; Clausse, María; Manazza, J.; Cassataro, Juliana; Giambartolomei, Guillermo H.; Coria, Lorena M.; Zylberman, Vanesa; Fossati, Carlos A.; Kjeken, Rune; Goldbaum, Fernando A.

doi:10.1016/j.vaccine.2009.08.097

Cited by 40 publications

(47 citation statements)

References 34 publications

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“…Although certain subunit vaccines have been demonstrated to be efficacious and useful (8,12), one of the most promising of the vaccine approaches, based on published results and effectiveness in the field, is use of a live, attenuated agent (13). Vaccines currently in use are derived from spontaneously occurring attenuated forms that arise randomly and that therefore do not provide a means to control the combination of defects that attenuate survival (13).…”

Section: Discussionmentioning

confidence: 99%

TheBrucella abortusPhosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice

Trant¹,

Lacerda²,

Carvalho³

et al. 2010

Infect Immun

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Section: Discussionmentioning

confidence: 99%

TheBrucella abortusPhosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice

Trant¹,

Lacerda²,

Carvalho³

et al. 2010

Infect Immun

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“…Microarrays done under different experimental conditions, i.e., infection times and cell types, performed in concert with qRT-PCR will fill in the gaps of what we know about the expression of our epitope-containing proteins. Future work will investigate the kinetic expression of epitope-containing proteins over the course of infection, Considering that long-term protection to Brucella is either not complete or largely not addressed in studies of single whole-protein and polymeric vaccines, we do not anticipate that complete protection might be engendered by the single epitopes presented here (1,17,31). Rather, these data allow the development of tetramers to track the Brucella-specific CD8 ϩ T-cell response, as well as lay the groundwork for potential multivalent peptide vaccines of the future.…”

Section: Discussionmentioning

confidence: 99%

DiscordantBrucella melitensisAntigens Yield Cognate CD8⁺T Cells In Vivo

et al. 2010

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Brucella spp. are intracellular bacteria that cause the most frequent zoonosis in the world. Although recent work has advanced the field of Brucella vaccine development, there remains no safe human vaccine. In order to produce a safe and effective human vaccine, the immune response to Brucella spp. requires greater understanding. Induction of Brucella-specific CD8 ؉ T cells is considered an important aspect of the host response; however, the CD8 ؉ T-cell response is not clearly defined. Discovering the epitope containing antigens recognized by Brucella-specific CD8 ؉ T cells and correlating them with microarray data will aid in determining proteins critical for vaccine development that cover a kinetic continuum during infection. Developing tools to take advantage of the BALB/c mouse model of Brucella melitensis infection will help to clarify the correlates of immunity and improve the efficacy of this model. Two H-2 d CD8 ؉ T-cell epitopes have been characterized, and a group of immunogenic proteins have provoked gamma interferon production by CD8 ؉ T cells. RYCINSASL and NGSSSMATV induced cognate CD8 ؉ T cells after peptide immunization that showed specific killing in vivo. Importantly, we found by microarray analysis that the genes encoding these epitopes are differentially expressed following macrophage infection, further emphasizing that these discordant genes may play an important role in the pathogenesis of B. melitensis infection.Brucellosis is the world's most common zoonosis, with more than half a million new human infections each year (44). Brucellosis has been endemic to the Mediterranean and Middle East since ancient times, since carbonized cheese and skeletal remains in Pompeii show evidence of Brucella spp. (8). Evidence of brucellosis also exists in the skeleton of a 2.4-to 2.8-million-year-old hominid (16). In areas of endemicity, domestic animal brucellosis severely affects regional economies, and vaccination campaigns cannot always reach nomadic herders. Human infections occur in these regions mainly from the ingestion of infected animal products, including unpasteurized milk and fresh cheeses (14). Antibiotic treatment exists but is costly and prolonged, lasting at least 6 weeks in moderate cases, and it may extend for years depending on complications that arise. Even after treatment, PCR data have revealed that low levels of bacteria are detectable years after the resolution of symptoms, and relapses occur in 5 to 30% of cases (20,30,55,62). In areas where brucellosis is endemic, prevention of infection via vaccine would be far more cost-effective than the regimen of antibiotics suggested by the World Health Organization (WHO). Unfortunately, this disease flies below the radar of many of the major world health agencies, and the problem is compounded by frequent misdiagnosis and under-reporting (15,20).Although brucellosis is eradicated from food sources here, in the post-Gulf War United States, awareness was raised to fund vaccine research concerning potential biological weapons.Brucella melit...

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“…antibodies showed bactericidal activity against B. ovis in the presence of an homologous source of complement (Estein et al, 2009). In this work, sera collected at week 10 after first immunization showed significant bactericidal activity against B. canis (p<0.001), although lower than positive control serum (infected dog) (Figure 3).…”

Section: Resultsmentioning

confidence: 53%

“…canis challenge in BALBc mice (Clausse et al, 2013) and also conferred protection in mice and rams against B. ovis (the other naturally rough strain of the genus) (Cassataro et al, 2007;Estein et al, 2009). The immunization strategies induced a mixed Th1/Th2 response and high specific IgG titers which did not interfere in current serological test (RSAT: Rapid Slide Agglutination Test) for diagnosis of canine brucellosis (Clausse et al, 2013).…”

Section: Dna Vaccines Codifying For Eight Different Proteins Against mentioning

confidence: 99%

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Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs

Clausse

Díaz

Pardo

et al. 2017

Veterinary Immunology and Immunopathology

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M., Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs.Veterinary Immunology and Immunopathology http://dx.doi.org/10. 1016/j.vetimm.2016.11.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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The polymeric antigen BLSOmp31 confers protection against Brucella ovis infection in rams

Cited by 40 publications

References 34 publications

TheBrucella abortusPhosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice

TheBrucella abortusPhosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice

DiscordantBrucella melitensisAntigens Yield Cognate CD8⁺T Cells In Vivo

Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs

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The polymeric antigen BLSOmp31 confers protection against Brucella ovis infection in rams

Cited by 40 publications

References 34 publications

TheBrucella abortusPhosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice

TheBrucella abortusPhosphoglycerate Kinase Mutant Is Highly Attenuated and Induces Protection Superior to That of Vaccine Strain 19 in Immunocompromised and Immunocompetent Mice

DiscordantBrucella melitensisAntigens Yield Cognate CD8+T Cells In Vivo

Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs

Contact Info

Product

Resources

About

DiscordantBrucella melitensisAntigens Yield Cognate CD8⁺T Cells In Vivo