The polymorphism of rs6505162 in the MIR423 coding region and recurrent pregnancy loss

Su, Xing; Hu, Yi; Liu, Ying; Cao, Jingli; Wang, Xueqin; Ma, Xu; Xia, Hong-Fei

doi:10.1530/rep-15-0007

Cited by 34 publications

(22 citation statements)

References 33 publications

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“…However, in our study, we did not find a statistically significant association between miR-423 genotypes and RPL risk. Notably, a recent study in the Han Chinese population also failed to identify an association between the miR-423 genotypes and RPL risk; however, the study found an association between miR-423 alleles and RPL, with functional evidence showing that the minor A allele contributed to an increased expression of mature miR-423 [7]. An explanation for the different results may be related to the different allele frequencies.…”

Section: Discussionmentioning

confidence: 99%

“…Another study reported an association between two pre-miRNA polymorphisms (miR-196a2 and miR-499) and the occurrence of RPL in Korean females [9], which was supported in Iranian women [6]. The most recent study identified a polymorphism in the coding region of miR-423 contributing to an increase in the expression of mature miR-423 associated with RPL in the Han Chinese population [7]. Several miRNAs that are considered important during pregnancy were chosen for this study because of their elevated expression ( miR-27a ), decreased expression in the endometrium and in trophoblasts ( miR-423 ), lower expression during endometriosis ( miR-449b ), and involvement in pregnancy loss via the p53 network ( miR-605 ) [10–13].…”

Section: Introductionmentioning

confidence: 96%

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miR-27a and miR-449b polymorphisms associated with a risk of idiopathic recurrent pregnancy loss

Rah

Chung

et al. 2017

PLoS ONE

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ObjectiveMicroRNAs (miRNAs) regulate gene expression during the peri-implantation period. The purpose of this study was to investigate whether genetic polymorphisms in the four miRNAs associated with fetal or placental development play roles in the development of idiopathic recurrent pregnancy loss (RPL) in Korean females.Study designA case-control study involving 225 controls and 387 women with at least two consecutively recurrent pregnancy losses between 1999 and 2012 was performed. The genotypes of the four miRNA polymorphisms, including miR-27a rs895819, miR-423 rs6505162, miR-449b rs10061133, and miR-605 rs2043556, were analyzed by the polymerase chain reaction-restriction fragment length polymorphism assay. Odds ratios and 95% confidence intervals were estimated using multivariate analyses after maternal age adjustments. The relationships between each of the four microRNA genotypes and each of the six clinical parameters of the RPL patients (plasma homocysteine and folate levels, natural killer cell number, platelet count, prothrombin time, and, activated partial thromboplastin time) were analyzed using multiple linear regression analyses.ResultsOur results suggest that weak associations between decreased RPL risk and the genotypes of miR-27a (AG and AG+GG), combination genotype of miR-27a/miR-423 (AG/GC), and haplotypes of miR-27a/miR-423/miR-449b/miR-605 (G-C-A-G) and miR-27a/miR-449b/miR-605 (G-A-G), whereas weak associations between increased RPL risk and genotypes of miR-449b (GG and AG+GG), combination genotypes of miR-423/miR-449b (CC/GG and CA/AG), miR-449b/miR-605 (AG/AG), haplotypes of miR-27a/miR-423/miR-449b/miR-605 (A-C-G-A, A-A-A-G, and G-C-G-G), miR-27a/miR-423/miR-449b (A-C-G), miR-27a/miR-449b/miR-605 (A-A-G, A-G-A, and G-G-G), miR-423/miR-449b/miR-605 (C-G-G and A-A-G), and miR-423/miR-449b (C-G and A-A). The genotypes of miR-27a (AG and AG+GG) also showed significant contributions to the prediction of folate levels in RPL patients.ConclusionsThe study showed associations between miRNA polymorphisms (miR-27a rs895819 and miR-449b rs10061133) and RPL development, and between the miRNA polymorphism (miR-27a rs895819) and plasma folate levels.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

miR-27a and miR-449b polymorphisms associated with a risk of idiopathic recurrent pregnancy loss

Rah

Chung

et al. 2017

PLoS ONE

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“…To our knowledge, our study provides the first piece of evidence that links the functional SNPs in pre‐miR‐323b to RPL susceptibility, although increasing evidences indicated that SNPs in miRNAs were identified to have a great relationship with diseases (Hu et al, ; Jazdzewski et al, ; Saunders et al, ; Su et al, ). Here, we had identified two variant alleles in pre‐miR‐323b that led to the altered production of miR‐323b , which was supposed to be associated with an increased risk for RPL in Han–Chinese population.…”

Section: Discussionmentioning

confidence: 86%

Haplotype‐based association of two SNPs in miR‐323b with unexplained recurrent spontaneous abortion in a Chinese Han population

Wang

Liu

et al. 2018

Journal Cellular Physiology

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Single nucleotide polymorphisms (SNPs) in miRNAs are associated with the risk to development of certain human diseases and affect the regulatory capacity of miRNAs. However, the relationship between miRNAs polymorphisms and recurrent pregnancy loss (RPL) is still largely unknown. Our study found that one SNP rs56103835 T>C in miR-323b coding region was associated with the increase risk of human unexplained RPL (URPL), but no differences were found in another SNP rs75330474 C>T. However, in two-locus haplotype analysis, T-C haplotype was associated with an increased risk of URPL. The level of mature miR-323b was obviously up-regulated in cells transfected with T-C haplotype. T-C haplotype inhibited HTR-8/SVneo cells proliferation and migration and promoted cells apoptosis. Further experiments identified that paired-box 8 (Pax8) was a functionally relevant target of miR-323b, and its expression was reversely regulated by miR-323b. Besides, the expressions of Pax8 in villous chorionic tissues from URPL patients were lower than controls, contrary to the high expression of miR-323. More importantly, dual-luciferase assay indicated T-C haplotype, increasing miR-323b expression, could down-regulated Pax8 expression. Collectively, our data suggest that T-C haplotype in pre-miR-323b may aggravate the risk of developing URPL and influence the level of mature miR-323b and its target gene Pax8.

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“…(35) In the polymorphism of pre-MIR423 rs6505162, genetic mutations, C to A transition, inhibited the function of HEC-1b cell proliferation and migratory. (36) The abduction of EndMT progression accelerating cell proliferation and migratory capacity was caused by KD serum in endothelial cells, which could be one of the pathogenesis in KD. (14) On the basis of the series of articles above, there is a clear connection between miRNA-423 and coronary artery and coronary collateral.…”

Section: Discussionmentioning

confidence: 99%

The rs6505162 C>A polymorphism in themiRNA-423gene exhibits a protective element of coronary artery in a southern Chinese population with Kawasaki disease

Jiang

et al. 2019

Preprint

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BackgroundManifesting as acute rash, fever and vasculitis, belonging to autoimmune syndrome, Kawasaki disease(KD) is prone to occur in infants and young children. Males and females is affected by KD at a ratio of 1.4 to 1.7: 1. KD is known to own many common clinical manifestations and complications, like coronary artery lesion(CAL) and coronary artery aneurysm(CAA). Polymorphisms of the rs6505162 locus in themiRNA-423gene are associated with enhancive susceptibility to coronary artery disease and the alterations of the four cytokines IL-4., IL-10, IL-21, IL-22 in the early stages of diabetes. However, no researcher has reported whether rs6505162 is related to KD susceptibility or no. Therefore, we carried out the trial concentrating on the connection betweenmiRNA-423rs6505162 C>A polymorphism and KD susceptibility.MethodsTo obtain the genotypes of rs6505162inobjects enrolled by 532 KD children and 623 control, we applied Taqman real-time PCR and all statistical analyses was carried out by SAS.ResultsThe comparison between all cases and all controls hinted that the rs6505162C>A polymorphism has no relationship with KD susceptibility. Nevertheless, a subgroup analysis revealed that the CA/AA genotypes of rs6505162 could reduce the occurrence of CAA (Adjusted age and gender odds ratio=1.30, 95%CI=1.02-1.67,P=0.037) and CAL (Adjusted OR=1.56, 95%CI=1.19-2.03,P=0.001)in KD patients.ConclusionOur final results stated clearly thatmiRNA-423rs6505162 polymorphism appears to be a protective element of CAL and CAA in southern Chinese suffers with KD.

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The polymorphism of rs6505162 in the MIR423 coding region and recurrent pregnancy loss

Cited by 34 publications

References 33 publications

miR-27a and miR-449b polymorphisms associated with a risk of idiopathic recurrent pregnancy loss

miR-27a and miR-449b polymorphisms associated with a risk of idiopathic recurrent pregnancy loss

Haplotype‐based association of two SNPs in miR‐323b with unexplained recurrent spontaneous abortion in a Chinese Han population

The rs6505162 C>A polymorphism in themiRNA-423gene exhibits a protective element of coronary artery in a southern Chinese population with Kawasaki disease

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