The polymorphisms of MyoD1 gene in Manych Merino sheep and its influence on body conformation traits

Abstract

Candidate genes associated with meat productivity often affect the performance of myostatin and muscle development in general. The MyoD1 gene is a member of the myogenic regulatory factor (MRF) family and plays a key role in the differentiation of skeletal muscle cells in vertebrates. We investigated the structure of the MyoD1 gene. The effect of polymorphisms on meat production was studied in the Russian sheep breed Manych Merino. To detect alleles, we used NimbleGen sequencing technology (Roche, USA). In the Manych Merino breed, we found 14 single nucleotide polymorphisms (SNPs) associated with substitutions, all in the second exon, namely, c.244C>T, c.246G>T, c.253G>T, c.259G>C, c.261C>T, c.269C>G, c.274C>A, c.276C>G, c.277C>A, c.279C>T, c.281C>A, c.287C>A, c.325T>C, and c.483C>T. All of these SNPs, except from c.325T>C, were detected here for the first time. The Manych Merino breed had 13 substitutions that were present as homozygous type. Only SNP c.325T>C occurred as the wild type of homozygotes and heterozygotes in the ratio 2:1. We found that SNP c.325T>C was associated with some vital body parameters, including parameters of height and croup measurement. Thus, the determination of allelic variants of the MyoD1 gene may be used in marker assisted genetic selection schemes.

Insights into the influence of diet and genetics on feed efficiency and meat production in sheep

“…Polymorphisms in the intron region of IGF1 was found to be associated with carcass traits such as length of carcass and rib yield (Meira et al., 2019). Two other studies report SNPs in the intron 1 region to have associations with sheep body weight (Trukhachev, Skripkin, et al., 2016) and carcass traits (Su et al., 2014). Two IGF1 genotypes namely A 2 and B 2 were detected by Li et al.…”

Growth Hormone (GH) Gene Polymorphism and Its Association with Meat Productivity in Two Rough Wool Sheep Breeds Grown in Russia's Dry Zone

“…In recent years, a number of reports have appeared that noted the relationship between the different gene polymorphisms and the meat production of sheep bred in Russia (Gorlov et al 2016;Trukhachev et al 2016Trukhachev et al , 2017Gorlov et al 2018b). The purpose of these studies was to enhance the level and quality of meat production, as well as reduce the cost of feed per unit of production in Russian sheep breeding.…”