The porcine <i>SRY</i> promoter is transactivated within a male genital ridge environment

Daneau, Isabelle; Pilon, Nicolas; Boyer, Alexandre; Behdjani, Ramin; Overbeek, Paul A.; Viger, Robert S.; Lussier, Jacques G.; Silversides, David W.

doi:10.1002/gene.10106

Cited by 25 publications

(33 citation statements)

References 41 publications

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“…Under this hypothesis, one of the roles of SRY will be to inhibit PISRT1, allowing the up-regulation of SOX9 in the XY undifferentiated gonads. Interestingly, it has been shown recently that the pig SRY promoter can be trans-activated by SOX9 (Daneau et al, 2002), via a SOX9 potential binding site identified at -205 bp of the pig promoter sequence. This SOX9 binding site is conserved in the 5) flanking sequences of goat, bovine and human SRY genes but not in the mouse gene (Daneau et al, 2002;and personal observation).…”

Section: Expression Profiles Of Foxl2 and Pisrt1 In The Mousementioning

confidence: 99%

“…Interestingly, it has been shown recently that the pig SRY promoter can be trans-activated by SOX9 (Daneau et al, 2002), via a SOX9 potential binding site identified at -205 bp of the pig promoter sequence. This SOX9 binding site is conserved in the 5) flanking sequences of goat, bovine and human SRY genes but not in the mouse gene (Daneau et al, 2002;and personal observation). Taking into account this observation, a regulatory loop appears also to exist in higher mammals, SOX9 activates SRY which represses PISRT1 itself repressing SOX9 (Fig.…”

Section: Expression Profiles Of Foxl2 and Pisrt1 In The Mousementioning

confidence: 99%

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Expression studies of the PIS-regulated genes suggest different mechanisms of sex determination within mammals

Pannetier

Servel

Cocquet

et al. 2003

Cytogenet Genome Res

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In mammals, the Y-located SRY gene is known to induce testis formation from the indifferent gonad. A related gene, SOX9, also plays a critical role in testis differentiation in mammals, in birds and reptiles. It is now assumed that SRY acts upstream of SOX9 in the sex determination cascade, but the regulatory link which should exist between these two genes remains unknown. Studies on XX sex reversal in polled goats (PIS mutation: Polled Intersex Syndrome) have led to the discovery of a female-specific locus crucial for ovarian differentiation. This genomic region is composed of at least two genes, FOXL2 and PISRT1, which share a common transcriptional regulatory region, PIS. In this review, we present the expression pattern of these PIS-regulated genes in mice. The FOXL2 expression profile of mice is similar to that described in goats in accordance with a conserved role of this ovarian differentiating gene in mammals. On the contrary, the PISRT1 expression profile is different between mice and goats, suggesting different mechanisms of the primary switch in the testis determination process within mammals. A model based on two different modes of SOX9 regulation in mice and other mammals is proposed in order to integrate our results into the current scheme of gonad differentiation.

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Section: Expression Profiles Of Foxl2 and Pisrt1 In The Mousementioning

confidence: 99%

Section: Expression Profiles Of Foxl2 and Pisrt1 In The Mousementioning

confidence: 99%

Expression studies of the PIS-regulated genes suggest different mechanisms of sex determination within mammals

Pannetier

Servel

Cocquet

et al. 2003

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…Recent studies of the pig SRY gene have shown that SOX9 may be involved in the initiation of SRY transcription and possible feedback amplification of the SRY gene (Daneau et al, 2002). This interaction was found to occur via a motif conserved in humans and bovine, which is present 205 bp upstream of the translational start site of SRY in the pig (Daneau et al, 2002).…”

Section: Sox9 Targets In the Sex Determination Pathwaymentioning

confidence: 99%

“…It was found that in the XX transgenic male mice, GFP was detectable indicating that SRY promoter activity does not require factors on the Y chromosome or SRY protein itself. Rather, SRY promoter activity requires factors found within a testicular environment, regardless of the genotype (Daneau et al, 2002).…”

Section: Sox9 Targets In the Sex Determination Pathwaymentioning

confidence: 99%

“…This interaction was found to occur via a motif conserved in humans and bovine, which is present 205 bp upstream of the translational start site of SRY in the pig (Daneau et al, 2002). In these studies, Odsex XX embryos containing a transgene with GFP under the control of pig SRY 5) flanking sequence were generated.…”

Section: Sox9 Targets In the Sex Determination Pathwaymentioning

confidence: 99%

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Turning on the male – SRY, SOX9 and sex determination in mammals

Knower

Kelly

Harley³

2003

Cytogenet Genome Res

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The decision of the bi-potential gonad to develop into either a testis or ovary is determined by the presence or absence of the Sex-determining Region gene on the Y chromosome (SRY). Since its discovery, almost 13 years ago, the molecular role that SRY plays in initiating the male sexual development cascade has proven difficult to ascertain. While biochemical studies of clinical mutants and mouse genetic models have helped in our understanding of SRY function, no direct downstream targets of SRY have yet been identified. There are, however, a number of other genes of equal importance in determining sexual phenotype, expressed before and after expression of SRY. Of these, one has proven of central importance to mammals and vertebrates, SOX9. This review describes our current knowledge of SRY and SOX9 structure and function in the light of recent key developments.

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Electrical properties of conductive adhesives as affected by particle compositions, particle shapes, and oxidizing temperatures of copper powders in a polymer matrix

Lin

Chiu

2004

J of Applied Polymer Sci

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The effects of particle compositions, particle shapes, and oxidation temperatures on the electrical properties of conductive adhesives have been investigated. Silver-coated copper powders and uncoated copper powders with spherical and flake-shaped particles are oxidized at temperatures such as 30, 175, 240, 300°C and 350°C for 2 h and dispersed in an epoxy matrix. The results of this study indicate that the electrical properties of the conductive adhesives are strongly affected by the particle compositions and oxidation temperatures and only slightly affected by the particle shapes. Silver-coated copper powders show significantly greater oxidation resistance than uncoated copper powders. To understand how silver-coated copper powders show such oxidation resistance, they are analyzed by the techniques of thermogravimetric analysis (TGA), X-ray diffraction (XRD), and Auger spectroscopy to observe how metal oxides such as AgO, Cu 2 O, and CuO affect the electrical properties of the conductive adhesives.

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The porcine SRY promoter is transactivated within a male genital ridge environment

Cited by 25 publications

References 41 publications

Expression studies of the PIS-regulated genes suggest different mechanisms of sex determination within mammals

Expression studies of the PIS-regulated genes suggest different mechanisms of sex determination within mammals

Turning on the male – SRY, SOX9 and sex determination in mammals

Electrical properties of conductive adhesives as affected by particle compositions, particle shapes, and oxidizing temperatures of copper powders in a polymer matrix

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