The porphyrin–fullerene nanoparticles to promote the ATP overproduction in myocardium: 25Mg2+-magnetic isotope effect

Rezayat, Seyed Mahdi; Boushehri, Seyed Vahid Shetab; Salmanian, Bahram; Omidvari, Amir Houshang; Tarighat, S.; Esmaeili, Sara; Sarkar, Saeed; Amirshahi, Nima; Alyautdin, R. N.; Орлова, М. А.; Trushkov, Igor V.; Бучаченко, А. Л.; Liu, K.C.; Kuznetsov, Dmitry A.

doi:10.1016/j.ejmech.2008.07.030

Cited by 56 publications

(47 citation statements)

References 33 publications

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“…Of particular interest is the treatment of ischemic conditions such as stroke and heart disease, where reduced oxygen supply to the affected tissue leads to a severe reduction in the cell's ability to produce ATP. Indeed, Buchachenko and co-workers have been involved in developing a delivery system on the basis of a porphyrin adduct of C 60 (21), and positive results have been reported in preclinical trials on mammalian heart muscle (22,27). The 10% natural abundance of 25 Mg and the requirement for very high Mg 2þ concentrations should limit the influence of the magnetic isotope effect (MIE) and associated magnetic field effects under normal physiological conditions, but BK assert that effects could be significant because of natural local fluctuations in Mg ion concentrations (17).…”

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confidence: 99%

Reexamination of magnetic isotope and field effects on adenosine triphosphate production by creatine kinase

Crotty

Silkstone

Poddar

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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The influence of isotopically enriched magnesium on the creatine kinase catalyzed phosphorylation of adenosine diphosphate is examined in two independent series of experiments where adenosine triphosphate (ATP) concentrations were determined by a luciferase-linked luminescence end-point assay or a real-time spectrophotometric assay. No increase was observed between the rates of ATP production with natural Mg, 24 Mg, and 25 Mg, nor was any significant magnetic field effect observed in magnetic fields from 3 to 1,000 mT. Our results are in conflict with those reported by Buchachenko et al. [J Am Chem Soc 130:12868–12869 (2008)], and they challenge these authors’ general claims that a large (two- to threefold) magnetic isotope effect is “universally observable” for ATP-producing enzymes [Her Russ Acad Sci 80:22–28 (2010)] and that “enzymatic phosphorylation is an ion-radical, electron-spin-selective process” [Proc Natl Acad Sci USA 101:10793–10796 (2005)].

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confidence: 99%

Reexamination of magnetic isotope and field effects on adenosine triphosphate production by creatine kinase

Crotty

Silkstone

Poddar

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…arrest the degenerative changes of nerve fibre pathology [4]. Oxidative stress mechanisms by induction of mitochondrial dysfunction, decrease in adenosine triphosphate (ATP) and neural death play important roles in peripheral neuropathy [6][7][8]. Our recent study also indicated the usefulness of PMC16 in DN in terms of improvement of oxidative stress and mitochondrial ATP production [9].…”

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confidence: 99%

“…These nanoparticles are a novel pharmaceutical nanotool based on the porphyrin-attached fullerene-C60 'ball' (porphylleren-MC16 or PMC16) [6]. Regarding accumulation of this nanoparticle in neural mitochondria [6,10], we aimed to analyse the efficacy of PMC16 on morphological …”

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confidence: 99%

Protective Effect of Magnesium-25 Carrying Porphyrin-Fullerene Nanoparticles on Degeneration of Dorsal Root Ganglion Neurons and Motor Function in Experimental Diabetic Neuropathy

Hosseini

Abdollahi

Hassanzadeh

et al. 2011

Basic &Amp; Clinical Pharmacology &Amp; Toxicology

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Diabetic neuropathy (DN) is the most common peripheral neuropathy and long-term complication of diabetes. In view of the pathological basis for the treatment of DN, it is important to prevent nerve degeneration. Most of the current treatment strategies are symptomatic therapies. In this study, we evaluated the effectiveness of magnesium-25, carrying porphyrin-fullerene nanoparticles, on diabetes-induced neuropathy. Previous studies have suggested that dorsal root ganglion (DRG) neurons comprise a specific target and may be responsible for the known complications of DN. Experimental DN was induced by intraperitoneal injection of streptozotocin (STZ) (45 mg ⁄ kg). Different forms of magnesium including 25 24 Mg-PMC16 and MgCl 2 were administered intravenously in equal dose (0.5 LD 50 ) at 48-hr intervals before STZ injection. Peripheral nerves were studied after 2 months of diabetes in groups using qualitative approaches, morphometric analysis of DRG neurons and motor function tests. We showed that STZ-induced DN caused morphological abnormalities in DRG neurons comprising changes in area, diameter and number of A and B cells as well as motor dysfunction in DN. Moreover, our findings indicated that administration of 25 Mg-PMC16 as a magnetic form of Mg improved morphological abnormalities and motor dysfunctions significantly, whereas other forms of Mg were ineffective.Diabetic neuropathy (DN) is the most common late complication of diabetes showing an increasing prevalence [1]. Sixty per cent of patients with diabetes show evidence of peripheral nerve disease [2]. Similar to other diabetes complications, DN has been ascribed to hyperglycaemia and subsequent metabolic abnormalities such as increased polyol pathway activity leading to the accumulation of sorbitol and fructose, imbalances in NADP ⁄ NAD + , auto-oxidation of glucose causing the formation of reactive oxygen species, advanced glycation end-products produced by nonenzymatic glycation of proteins, inappropriate activation of protein kinase C (PKC) and a deficit of neurotrophic supports [3]. The pathology of DN includes axonal atrophy, demyelination, loss of nerve fibres and decreased regeneration of nerve fibres [4]. Abnormalities of peripheral nerve function are typical late complications of diabetes. However, by applying streptozotocin (STZ) injections in rats, such a spectrum of disease characteristics can be seen much earlier, i.e. only several weeks after induction of diabetes [5].Based on these observations, pharmacological therapies including aldose reductase inhibitors, antioxidant drugs, aminoguanidine and neurotrophic factors have been used in the treatment of DN [4]. Notwithstanding their importance, these treatments are solely symptomatic therapies, because peripheral nerve tissue damage cannot be reversed. Thus, early diagnosis of DN followed by a stringent pharmacological therapy may be effective to prevent the progression of DN, i.e. arrest the degenerative changes of nerve fibre pathology [4]. Oxidative stress mechanisms by induc...

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“…Both nucleophylic and ion-radical mechanisms coexists independently, they are about to contribute additively into the common ATP synthesis multi-compartment biochemical system. The former dominates in vivo, the latter may be turned on artificially by the targeted delivering of Mg (or,even better,25 Mg) to the heart muscle in order to hyper-stimulate the presuppressed ATP synthesis rate and hence to prevent some pathologies related to the ATP deficiency (Amirshahi et al, 2008 a,b;Rezayat et al, 2009) . This present work deals with an effort to reveal whether or not the enzymatic ATP synthesis is to be also specifically affected by magnetic calcium ions.…”

Section: Introductionmentioning

confidence: 99%

A specific role of 43Ca in the enzymatic ATP synthesis

Бучаченко¹,

Breslavskaya²,

Kuznetsov³

et al. 2011

AJBMS

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This is the first report ever on the nuclear spin selective 43 Ca 40 Ca isotope effect expressed in the Calcium affected enzymatic ATP synthesis. Once the creatine kinase (CK) catalytic sites were loaded with 43 Ca 2+ , the resulting enzyme activity goes up (2.0±0.3 -fold) compared to the CKdirected ATP synthesis rate occurred in the presence of nonmagnetic, spinless, 40 Ca nuclei. The effect manifests itself predominantly within a high concentration range (Calcium chloride,40 mM) indicating that the ATP synthesis is a spin-selective ion-radical process. Primary reaction of this path is an electron transfer between the reaction partners, С a(H2O)n 2+ ( n ≤ 4) and Ca(ADP) 3-, generating an ion-radical pair where the singlet -triplet spin conversion takes place as a result of the nuclear spin selectivity and a consequent magnetic isotope effect. This, in turn, makes an essential impact on the final reaction's ATP yield. To the contrast, there is no any isotope effect found within a low Ca 2+ concentration range showing that the ATP synthesis proceeds in a most common nucleophylic way. The submolecular mechanisms of phenomena described and their possible applied significance are under discussion.

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The porphyrin–fullerene nanoparticles to promote the ATP overproduction in myocardium: 25Mg2+-magnetic isotope effect

Cited by 56 publications

References 33 publications

Reexamination of magnetic isotope and field effects on adenosine triphosphate production by creatine kinase

Reexamination of magnetic isotope and field effects on adenosine triphosphate production by creatine kinase

Protective Effect of Magnesium-25 Carrying Porphyrin-Fullerene Nanoparticles on Degeneration of Dorsal Root Ganglion Neurons and Motor Function in Experimental Diabetic Neuropathy

A specific role of 43Ca in the enzymatic ATP synthesis

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