The Possibilities of Cell Technologies in the Treatment of Cicatricial Lesions of the Vocal Folds

Свистушкин, В; Старостина, С. В.; Люндуп, Алексей Валерьевич; Дедова, Мария Георгиевна; Будейкина, Лилия Сергеевна; Свистушкин, М.; Крашенинников, Михаил Евгеньевич; Baranovskii, Denis S.

doi:10.15690/vramn586

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…The parameters of the inflicted defect and the time of the scar maturation were optimal, because, according to the literature data, the depth of a defect must exceed the actual thickness of the vocal fold's lamina propria, as a trauma to the deep layers unavoidably leads to the cicatricial changes with the subsequent deterioration of the vibratory characteristics. Three months is a sufficient time for the completion of the active processes of collagen organisation in the scar tissue (Svistushkin et al, 2016).…”

Section: Creation Of a Defect In The Vocal Foldsmentioning

confidence: 99%

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Свистушкин

Kotova

Shekhter

et al. 2019

Journal of Microscopy

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Summary Regenerative medicine opens new opportunities in the repair of cicatricial lesions of the vocal folds. Here, we present a thorough morphological study, with the focus on the collagen structures in the mucosa of the vocal folds, dedicated to the effects of stem cells on the vocal folds repair after cicatricial lesions. We used a conventional experimental model of a mature scar of the rabbit vocal folds, which was surgically excised with a simultaneous implantation of autologous bone marrow‐derived mesenchymal stem cells (MSC) into the defect. The restoration of the vocal folds was studied 3 months postimplantation of stem cells and 6 months after the first surgery. The collagen structure assessment included histology, immunohistochemistry and atomic force microscopy (AFM) studies. According to the data of optical microscopy and AFM, as well as to immunohistochemical analysis, MSC implantation into the vocal fold defect leads not only to the general reduction of scarring, normal ratio of collagens type I and type III, but also to a more complete restoration of architecture and ultrastructure of collagen fibres in the mucosa, as compared to the control. The collagen structures in the scar tissue in the vocal folds with implanted MSC are more similar to those in the normal mucosa of the vocal folds than to those of the untreated scars. AFM has proven to be an instrumental technique in the assessment of the ultrastructure restoration in such studies. Lay Description Regenerative medicine opens new opportunities in the repair of the vocal fold scars. Because collagen is a main component in the vocal fold mucosa responsible for the scar formation and repair, we focus on the collagen structures in the mucosa of the vocal folds, using a thorough morphological study based on histology and atomic force microscopy (AFM). Atomic force microscopy is a scanning microscopic technique which allows revealing the internal structure of a tissue with a resolution up to nanometres. We used a conventional experimental model of a mature scar of the rabbit vocal folds, surgically excised and treated with a mesenchymal stem cells transplant. Our morphological study, primarily AFM, explicitly shows that the collagen structures in the scarred vocal folds almost completely restore after the stem cell treatment. Thus, the modern microscopic methods, and especially AFM are instrumental tools for monitoring the repair of the vocal folds scars.

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Section: Creation Of a Defect In The Vocal Foldsmentioning

confidence: 99%

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Свистушкин

Kotova

Shekhter

et al. 2019

Journal of Microscopy

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“…Therefore, great interest arose to the techniques and approaches of regenerative medicine for the development of an efficient way of treatment for VF scar lesions [ 15 – 19 ]. In experimental studies, positive effects of cells from various sources have been demonstrated, such as the reduction in general fibrosis signs, level of inflammation, thickness of the scar tissue, restoration of the extracellular matrix architecture and the tissues’ mechanical parameters [ 20 – 24 ].…”

Section: Introductionmentioning

confidence: 99%

Vocal fold restoration after scarring: biocompatibility and efficacy of an MSC-based bioequivalent

Svistushkin,

Shpichka,

Bikmulina

et al. 2023

Stem Cell Res Ther

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Background There is growing interest to application of regenerative medicine approaches in otorhinolaryngological practice, especially in the framework of the therapy of vocal fold (VF) scar lesions. The used conservative and surgical methods, despite the achieved positive outcomes, are frequently unpredictable and do not result in the restoration of the VF’s lamina propria’s structure, which provides the mechanical properties necessary for vibration. In this connection, the aim of this study was to ascertain the safety and efficacy of a bioequivalent in the treatment of VF scars using a rabbit model of chronic damage. Methods The bioequivalent consisted of a hydrogel system based on a PEG-fibrin conjugate and human bone marrow-derived MSC. It was characterized and implanted heterotopically into rats and orthotopically into rabbits after VF scar excision. Results We showed that the fabricated bioequivalent consisted of viable cells retaining their metabolic and proliferative activity. While being implanted heterotopically, it had induced the low inflammatory reaction in 7 days and was well tolerated. The orthotopic implantation showed that the gel application was characterized by a lower hemorrhage intensity (p = 0.03945). The intensity of stridor and respiratory rate between the groups in total and between separate groups had no statistically significant difference (p = 0.96 and p = 1; p = 0.9593 and p = 0.97…1, respectively). In 3 days post-implantation, MSC were detected only in the tissues closely surrounding the VF defect. The bioequivalent injection caused that the scar collagen fibers were packed looser and more frequently mutually parallel that is inherent in the native tissue (p = 0.018). In all experimental groups, the fibrous tissue’s ingrowth in the adjacent exterior muscle tissue was observed; however, in Group 4 (PEG-Fibrin + MSC), it was much less pronounced than it was in Group 1 (normal saline) (p = 0.008). The difference between the thicknesses of the lamina propria in the control group and in Group 4 was not revealed to be statistically significant (p = 0.995). The Young’s modulus of the VF after the bioequivalent implantation (1.15 ± 0.25 kPa) did not statistically significantly differ from the intact VF modulus (1.17 ± 0.45 kPa); therefore, the tissue properties in this group more closely resembled the intact VF. Conclusions The developed bioequivalent showed to be biocompatible and highly efficient in the restoration of VF’s tissue.

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“…у взрослых и 3 мес. -у детей) антибактери-альной терапии как факта риска инфекций, вызванных резистентными возбудителями [16]. Вопросы при выборе антибиотика согласно стандарту ■ стартовый антибиотик, ■ доза антибиотика, ■ форма, ■ безопасность, ■ комплаентность, ■ вероятность соблюдения рекомендованной длитель-ности терапии.…”

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Rational choice of antibiotic therapy in acute otitis media in children

Бойкова¹,

Гаращенко²

2017

Med. sov.

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В статье рассматриваются вопросы распространенности и частоты заболевания острого среднего отита (ОСО) в детском возрасте, причины возникновения заболевания, особенности терапии, эмпирический подход к назначению антибактери-ального лечения, выбор антибактериального препарата. The article examines the prevalence and incidence of acute otitis media (AOM) in children, the causes of the disease, the specific therapies, empirical approach to the prescription of antibacterial drugs, and the choice of antibiotics.

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The Possibilities of Cell Technologies in the Treatment of Cicatricial Lesions of the Vocal Folds

Cited by 6 publications

References 30 publications

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Vocal fold restoration after scarring: biocompatibility and efficacy of an MSC-based bioequivalent

Rational choice of antibiotic therapy in acute otitis media in children

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