The possible impact of DNA‐binding chromodomain‐helicase 5 polymorphisms on male infertility: A case–control study

Abstract

Background Chromodomain‐helicase 5 (CHD5) is a conventional tumor‐suppressing gene in many tumors. The CHD5 gene, as a key factor in the chromatin density process during sperm maturation, can affect the risk of infertility. This study aimed to determine whether CHD5 variants contribute to the risk of male infertility. Methods Gene variants were identified using tetra primer‐ARMS‐PCR method on nonobstructive azoospermia and severe oligozoospermia in a case–control study. SPSS software 20 (SPSS Inc. Chicago, IL, USA) was used for data recording and statistical analysis. Results In the codominant pattern, the rs12067480 TT variant versus CC significantly increased the risk of disease, and also, in the recessive pattern, TT variant versus CC + CT and T allele versus C. The rs2273032 variant was associated with the risk of infertility in codominant pattern AA versus GG and recessive pattern AA versus GG + GA and allele. We discovered that the rs12067480 T and rs2273032 A alleles increase the risk of male infertility. Also, the interaction of the CT/GA, CT/AA, TT/GA genotypes and rs12067480T/rs2273032A and rs12067480T/rs2273032G haplotypes significantly increased the risk of infertility. Conclusions Our results suggest that the CHD5 gene polymorphisms contribute to the risk of male infertility. Our findings can be valuable in improving the diagnosis and treatment of infertility.

Possible involvement of three-stemmed pseudoknots in regulating translational initiation in human mRNAs

“…CHD5 serves as a chromatin remodeling protein that potentially plays a role in the formation of a nucleosome remodeling and deacetylation complex, contributing to the regulation of gene transcription. In various contexts, CHD5 is implicated as a potential tumor suppressor, in neuroblastomas, gliomas, and many common adult tumors [ 65 – 67 ].…”

“…In lung adenocarcinoma cell lines, knockdown of BPHL gene expression through RNA interference resulted in a decrease in tumor growth, colony formation, and metastasis, while promoting an increase in apoptosis [ 63 ]. CHD5 and CTDSPL possess tumor suppressor activities in many cancers, including neuroblastomas, gliomas, and Non-Small Cell Lung Cancer (NSCLC) [ 65 – 67 , 86 ]. SLIT2 plays crucial protective roles in various processes, including cell migration, immune response, vascular permeability, and angiogenesis during the development of the central nervous system (CNS).…”

“…These potential pseudoknots are found in the mRNAs of Malassezia restricta dynactin 4 (MRET_4255), chromodomain helicase DNA binding protein 5 (CHD5), CTD small phosphatase like (CTDSPL), mab-21 like 4 (MAB21L4), sodium voltage-gated channel beta subunit 1 (SCN1B), protein phosphatase 2 catalytic subunit beta (PPP2CB), MYCL proto-oncogene bHLH transcription factor (MYCL), kelch like family member 2 (KLHL2), dynein axonemal intermediate chain 1 (DNAI1), slit guidance ligand 2 (SLIT2), biphenyl hydrolase like (BPHL), transmembrane protein 181 (TMEM181), small integral membrane protein 10 like 2B (SMIM10L2B), and small ArfGAP2 (SMAP2). The protein products derived from these mRNAs serve crucial functional roles, with many of them directly linked to various diseases [ 62 – 67 ].…”