The possible involvement of kupffer cell-mediated hepatocytotoxicity in the pathogenesis of liver injuries

Abstract

The possible involvement of cell-mediated immune response to liver specific lipoprotein (LSP) in the pathogenesis of liver injury was investigated. The subjects were consisted of one patient with acute hepatitis, five cases with chronic active hepatitis and one case with chronic failure inactive hepatitis. When peripheral blood lymphocytes from these patients were cultured in the presence of LSP and lymphocyte transformation was determined by measuring the uptake of [3H]-thymidine into the acid-insoluble materials, positive blastogenesis was seen in three cases with chronic active hepatitis. Furthermore, when peripheral blood lymphocytes from halothane-induced cholestatic hepatitis were cultured with offending drug in the presence of LSP and lymphocyte transformation was determined, a positive blastogenesis was seen. The factor which activated Kupffer cells (KCAF), a kind of lymphokine, was also detectable in the culture medium of activated lymphocytes from four patients who showed positive blastogenesis by estimating [3H]-glucosamine incorporation into Kupffer cells. The macrophage activating factor (MAF) was detectable in culture medium of activated lymphocytes from three patients who showed positive blastogenesis by estimating [3H]-glucosamine incorporation into macrophages. Furthermore, the KCAF-activated Kupffer cells and MAF-activated macrophages were shown to be cytotoxic for the isolated liver cells causing marked inhibition of albumin synthesis. The observations suggest that Kupffer cell-mediated cytotoxicity and macrophage-mediated cytotoxicity play a role in the pathogenesis of liver disease.

Murine Kupffer cells. Mononuclear phagocytes deficient in the generation of reactive oxygen intermediates.

“…The band at 33 K coresponded to apolipoprotein E, evidently a major product of resident KC. Additional bands at 34,35,40,55,58,68, 73, and 135 K were also shared between the two macrophage populations. The absence of a predominating band at 68 K in the KC population implied little secretion of albumin and thus minimal contamination with biosynthetically active hepatocytes.…”

“…A cell-derived protein that can suppress macrophage oxidative metabolism is currently under study (56,57). Indeed, the diminished ROI generation may prevent injury to hepatocytes or erythrocytes during KC's frequent phagocytic encounters (58,59). Distinctions among these possibilities may be aided by study of the NADPH oxidase of KC and its cytochrome b559 cofactor.…”