The possible mechanisms of copper resistance in the pathogen Pseudomonas syringae pathovars in stone fruit trees

Husseini, Ahmad; Akköprü, Ahmet

doi:10.1007/s12600-020-00828-1

Cited by 6 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…syringae is reported to be the most harmful bacterium that causes citrus blast and black pit in Tunisian orchards [23]. Various strategies to control bacterial plant diseases have been explored, including the use of copper products [43]. In addition, biological control using antagonistic bacteria is widely used for the management of bacterial diseases, either by directly stimulating the natural defenses in the host or by directly ensuring the biocontrol of bioaggressors [44].…”

Section: Discussionmentioning

confidence: 99%

Biological and Molecular Characterization of the Lytic Bacteriophage SoKa against Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast and Black Pit in Tunisia

Oueslati

Holtappels

Fortuna

et al. 2022

Viruses

View full text Add to dashboard Cite

Pseudomonas syringae pv. syringae (Pss), the causal agent of citrus blast and black pit lesion of lemon fruit, continues to cause serious damage in citrus production in Tunisia. Faced with the rapid emergence of the disease and the inefficiency of conventional control methods, an alternative strategy based on the use of bacteriophages was pursued in this study. The lytic Pss bacteriophage SoKa was isolated from soil collected from Tunisian citrus orchards. Analysis of the host range showed that SoKa was able to lyse seven other Pss strains. Interestingly, Pseudomonas syringae pv. porri, pathogenic to leek, could also be infected by SoKa. The activity of SoKa was maintained at pH values between 2 and 10, at temperatures between −80 and 37 °C; the phage could resist UV radiation at an intensity of 320 nm up to 40 min. Whole genome sequencing revealed that the Pseudomonas phage SoKa is a novel phage that belongs to the Bifseptvirus genus of the Autographiviridae family. The absence of virulence proteins and lysogeny-associated proteins encoded on the phage genome, its anti-biofilm activity, and the significant reduction of tissue necrosis in different fruit bioassays make SoKa potentially suitable for use in phage biocontrol.

show abstract

Section: Discussionmentioning

confidence: 99%

Biological and Molecular Characterization of the Lytic Bacteriophage SoKa against Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast and Black Pit in Tunisia

Oueslati

Holtappels

Fortuna

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…In the case of the copABCD operon identified in Pss, it is known that coded proteins occur in the periplasmic and outer membrane of the cell wall that confers Cu resistance by sequestrating the metal outside the cytoplasm (Cha and Cooksey, 1991). Recently, Husseini and Akköprü (2020) studied the mechanisms of Cu resistance in different P. syringae pathovars isolated from stone fruit trees, and found the presence of copABCD genes and the ability to produce siderophores, but not detected other mechanisms such as cusA gene. The role that plays the bacterial community associate with the bacterial canker disease in sweet cherry need to be addressed in future research, both in term of its role in the disease development and Cu resistance.…”

Section: Discussionmentioning

confidence: 99%

Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper

Beltrán

Osorio

Lemus

et al. 2021

Chil. j. agric. res.

View full text Add to dashboard Cite

The Chilean sweet cherry (Prunus avium (L.) L.) industry became the leading exporter worldwide. The bacterial canker is the most significant disease causing major economic losses. Pseudomonas syringae pv. syringae (Pss) is the only related pathovar recognized in Chile and it is mainly controlled with Cu-based antimicrobial compounds (CBAC). Soil contamination and the decreasing efficacy of CBACs by the emergence of Cu-resistant bacterial strains threaten the longterm sustainability of sweet cherry production. This study aimed at characterizing the bacterial community associated with canker infection injuries by assessing Cu resistance in sweet cherry orchards in the O'Higgins Region of Chile. Eighty bacteria isolates were obtained from tissue that presented bacterial canker symptoms from cherry trees. We assessed the production of fluorescent pigments in Fe-deficient media, presence of the housekeeping genes rpoD and cts, and presence of syringomycin-producing genes syrb and syrD. Their pathogenic ability was evaluated on immature sweet cherry fruits and Cu resistance was determined as the minimum inhibitory concentration (MIC) using CuSO4. Only five isolates were Cu-susceptible (MIC < 0.8 mM), while 75 isolates exhibited different levels of Cu resistance (MIC > 0.8 mM). At least one gene of the copABCD operon/or and its regulatory genes were detected in 17 isolates, suggesting that most isolates likely have different mechanisms of Cu tolerance. Six isolates were identified as Pss, presenting different degrees of Cu resistance, but all presented at least one feature of the copABCD operon. A bacterial community that presents high Cu-resistance, probably under control of diverse genetic mechanisms, decreases the efficacy of CBAC.

show abstract

Characterization and monitoring of copper tolerance of Pseudomonas syringae pv. tomato strains from tomato plants in Turkey

Horuz,

Haji Nour,

Aysan

2024

J Plant Dis Prot

View full text Add to dashboard Cite

The possible mechanisms of copper resistance in the pathogen Pseudomonas syringae pathovars in stone fruit trees

Cited by 6 publications

References 57 publications

Biological and Molecular Characterization of the Lytic Bacteriophage SoKa against Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast and Black Pit in Tunisia

Biological and Molecular Characterization of the Lytic Bacteriophage SoKa against Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast and Black Pit in Tunisia

Bacterial community associated with canker disease from sweet cherry orchards of central valley of Chile presents high resistance to copper

Characterization and monitoring of copper tolerance of Pseudomonas syringae pv. tomato strains from tomato plants in Turkey

Contact Info

Product

Resources

About