The Possible Protective Role of Crocin and Pumpkin Seed Oil against Lead-Induced Testicular Cytotoxicity in Adult Male Albino Rat.Hormonal, Histological and Immunohistochemical study.

Abstract

Background: Lead (Pb) disturbs of oxidant/antioxidant balance in testicular cells causing oxidative stress. Crocin (Cr) and pumpkin seed oil (PSO) have antioxidant effects and neutralize free radicals. Aim: This study was performed to assess the cytotoxicity of lead on testis and the possible protective effect of Cr and PSO. Methods: Forty eight healthy male rats were divided into six groups as follows: Control group, Cr group: each rat was received Crocin (200 mg/kg) given intraperitonealy (ip) once daily for 4 weeks, PSO group: each rat was orally received PSO (1 ml/kg) daily for 4 weeks , Pb group: each rat was received lead acetate, (8 mg/kg) ip daily for 4 weeks, (Cr+ Pb) group: each rat was received (200 mg/kg) crocin ip with 8 mg/kg Pb ip for 4 weeks and (PSO+ Pb) group: each rat was received PSO (1 ml/kg) orally in addition to ip lead acetate (8 mg/kg) daily for 4 weeks. The testicular tissues have been processed for a light microscopic study. Results: In Pb -treated rats, levels of FSH, LH, and testosterone significantly decreased. Decrease thickness of spermatogenic layers, mispresentation, Vacuolations, degeneration of spermatogenic cells, wide interstitial spaces, Weak PCNA and androgen receptor immunoexpression were observed. Both Cr and PSO treatment resulted in reversing Pb effects on testis presented in improvement in hormonal levels and histological architecture of most of seminiferous tubules with increase PCNA and androgen receptor immunoexpressions. Conclusions: Cr and PSO may have ameliorative effects after lead acetate toxicity on rat testis.