The possible protective role of omega 3 on liver of L thyroxine treated male albino rats. Histological and immunhistochemical study

Abstract

Background: Hyperthyroidism is a condition associated with oxidative damage of liver. Omega-3 fatty acids are effective in various medical conditions due to antioxidant, anti-inflammatory, anti-apoptotic and anti-fibrotic activities. Aim of the work: Here we evaluate the potential effects of omega-3 fatty acids on experimentally induced hyperthyroidism. Materials and methods: Thirty adult male rats were divided equally into 3 groups; Control group (G1) received saline. Hyperthyroid (GII) received L-thyroxine group 0.1µg/g/day intraperitoneal for 6 weeks. L-thyroxine+Omega-3 group (GIII) received L-thyoxine as previous group then 12% of eicosapentaenoic acid (EPA), and 18% of docosahexaenoic acid (DHA) for 4 weeks (from 3rd to 6th weeks). Blood samples were taken for detection of liver enzymes, serum T3 and TSH. Liver samples were taken for light and electron microscopic examination. Immunohistochemical staining of CD68, Caspase 3 and proliferating cell nuclear antigen (PCNA) were done. Results: L-thyroxine over dose decreased TSH, increased serum T3 and liver enzymes (ALT &AST). Also, it induced patches of cytoplasmic vacuolation, dense nuclei, vascular congestion and significant increase of collagen fibers. Electron microscopic changes revealed rarified cytoplasm and mitochondria with destructed cristae in most hepatocytes,. Significant increase of caspase3 and CD68 immunoexpression and significant decrease of (PCNA) immunoexpression were noticed. Concomitant treatment with omega 3 showed decreased liver enzymes and T3. Improvement of most morphological alterations in the hepatocytes was observed. Significant decrease of collagen fibers, caspase 3 and CD68 immunoexpression was observed and significant increase of PCNA immunoexpression. Conclusion: Combined treatment with omega 3 ameliorated most of L. thyroxine induced liver changes and serological parameters.

Effect of Induced Hyperthyroidism on Pancreas of Adult Female Albino Rats

“…Examination of Mallory's trichrome stained sections revealed that deposition of collagen fibers significantly increased in the connective tissue septa around the pancreatic acini, ducts and blood vessels of the hyperthyroid group. This was in accordance with Meligy et al (24) who examined the liver of hyperthyroid rats and observed significant increase in the collagen deposition and Hegazy et al (25) who found that hyperthyroidism accompanied with marked thickness in the capsule of the testes indicating that the collagen fibers deposition increased in the capsule as well as around the blood vessels. Safadi and Friedman (26) explained the increase of liver fibrosis that it might resulted from activation of stellate cells by ROS and subsequent increase in collagen synthesis and fibrogenesis.…”

Influence of hyperthyroidism on hepatic antioxidants and cytokines Levels: An Experimental Study

“…Hyperthyroidism is a condition associated with overproduction and secretion of thyroid hormones. Liver dysfunction has been reported in 37% to 77.9% of patients with hyperthyroidism (13). A well-known negative effect of hyperthyroidism is hepatic toxicity, which is characterized by an increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels (3,13).…”

“…Liver dysfunction has been reported in 37% to 77.9% of patients with hyperthyroidism (13). A well-known negative effect of hyperthyroidism is hepatic toxicity, which is characterized by an increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels (3,13). However, the possible mechanism between hyperthyroidism and hepatic dysfunction has not been fully explained (13).…”

“…A well-known negative effect of hyperthyroidism is hepatic toxicity, which is characterized by an increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels (3,13). However, the possible mechanism between hyperthyroidism and hepatic dysfunction has not been fully explained (13). In previous studies, some explanations have been proposed for liver dysfunction associated with hyperthyroidism.…”

Melissa officinalis extract palliates redox imbalance and inflammation associated with hyperthyroidism-induced liver damage by regulating Nrf-2/ Keap-1 gene expression in γ-irradiated rats

“…Consequently, a hyperthyroid state is associated with various chronic disorders such as cardiovascular diseases, diabetes and liver diseases [ 39 ], and impaired both the hepatic structure and function [ 4 , 40 ]. Additionally, the prevalence of liver dysfunction in patients with hyperthyroidism has been estimated to be between 37% and 77.9% [ 41 ].…”