The possible role of intracellular polyamines in mitochondrial metabolic regulation

Chaffee, R. R. J.; Arine, Robert M.; Rochelle, R. H.

doi:10.1016/0006-291x(79)90865-9

Cited by 23 publications

(8 citation statements)

References 15 publications

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“…The differential carbohydrate accumulation may also indicate that cell wall metabolism is altered to sustain the supply of C for respiration, which is consistent with the higher juice quality of these transgenic fruit (Mehta et al, 2002). The high respiratory activity in the transgenics reveals an in vivo role of polyamines in mitochondrial metabolic regulation, consistent with a previous proposal for Spm function in rat liver mitochondria (Chaffee et al, 1979).…”

Section: Discussion Higher Polyamines May Control Multiple Sites In Csupporting

confidence: 87%

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

et al. 2006

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Polyamines are ubiquitous aliphatic amines that have been implicated in myriad processes, but their precise biochemical roles are not fully understood. We have carried out metabolite profiling analyses of transgenic tomato (Solanum lycopersicum) fruit engineered to accumulate the higher polyamines spermidine (Spd) and spermine (Spm) to bring an insight into the metabolic processes that Spd/Spm regulate in plants. NMR spectroscopic analysis revealed distinct metabolite trends in the transgenic and wild-type/azygous fruits ripened off the vine. Distinct metabolites (glutamine, asparagine, choline, citrate, fumarate, malate, and an unidentified compound A) accumulated in the red transgenic fruit, while the levels of valine, aspartic acid, sucrose, and glucose were significantly lower as compared to the control (wild-type and azygous) red fruit. The levels of isoleucine, glucose, g-aminobutyrate, phenylalanine, and fructose remained similar in the nontransgenic and transgenic fruits. Statistical treatment of the metabolite variables distinguished the control fruits from the transgenic fruit and provided credence to the pronounced, differential metabolite profiles seen during ripening of the transgenic fruits. The pathways involved in the nitrogen sensing/signaling and carbon metabolism seem preferentially activated in the high Spd/Spm transgenics. The metabolite profiling analysis suggests that Spd and Spm are perceived as nitrogenous metabolites by the fruit cells, which in turn results in the stimulation of carbon sequestration. This is seen manifested in higher respiratory activity and up-regulation of phosphoenolpyruvate carboxylase and NADP-dependent isocitrate dehydrogenase transcripts in the transgenic fruit compared to controls, indicating high metabolic status of the transgenics even late in fruit ripening.

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Section: Discussion Higher Polyamines May Control Multiple Sites In Csupporting

confidence: 87%

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

et al. 2006

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“…As it has been previously reported the uptake of spermine by mitochondria is related to a large numbers of physiological effects. Spermine is able to stabilize mitochondrial membranes; it controls cation fluxes across the membranes, augments the respiratory control ratio and regulates the mitochondrial volume homeostasis (Saris et al 1969;Chaffee et al 1977Chaffee et al , 1978Chaffee et al , 1979Byczkowski et al 1982). In this regard, the polyamine modulates Ca 2?…”

Section: Role Of Spermine Cyclingmentioning

confidence: 99%

Bidirectional fluxes of spermine across the mitochondrial membrane

et al. 2013

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The polyamine spermine is transported into the mitochondrial matrix by an electrophoretic mechanism having as driving force the negative electrical membrane potential (DW). The presence of phosphate increases spermine uptake by reducing DpH and enhancing DW. The transport system is a specific uniporter constituted by a protein channel exhibiting two asymmetric energy barriers with the spermine binding site located in the energy well between the two barriers. Although spermine transport is electrophoretic in origin, its accumulation does not follow the Nernst equation for the presence of an efflux pathway. Spermine efflux may be induced by different agents, such as FCCP, antimycin A and mersalyl, able to completely or partially reduce the DW value and, consequently, suppress or weaken the force necessary to maintain spermine in the matrix. However this efflux may also take place in normal conditions when the electrophoretic accumulation of the polycationic polyamine induces a sufficient drop in DW able to trigger the efflux pathway. The release of the polyamine is most probably electroneutral in origin and can take place in exchange with protons or in symport with phosphate anion. The activity of both the uptake and efflux pathways induces a continuous cycling of spermine across the mitochondrial membrane, the rate of which may be prominent in imposing the concentrations of spermine in the inner and outer compartment. Thus, this event has a significant role on mitochondrial permeability transition modulation and consequently on the triggering of intrinsic apoptosis.

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“…CHAFFEE et al(1979)demonstrated that the polyamines would increase the respiratory control ratio and suppress the activity of the respiratory cycle in rat liver mitochondria.They also showed that the liver mitochondria from the heat-acclimated animals have a significantly greater elevation of respiratory control ratio than those from the control in the presence of polyamines (CHAFFEE et al,1978).Recently,a unique uncoupling mechanism in the form of a high-conductance of H+in the inner membrane of mitochondria without obligatory ATP synthesis has been proposed as being responsible for the remarkably high thermogenic capacity of brown adipose tissue (HEATON et al,1978). It might be argued that the polyamines effect a role as a factor tightening the respiratory control of this tissue,enhancing thermogenesis with a decrease in their levels.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies indicate that polyamines also affect the metabolic aspects of cellular activities (CHAFFEE et al, 1978(CHAFFEE et al, , 1979CLO et al, 1979;KITADA et al, 1979;LOCKWOOD and EAST, 1974). For example, changes in polyamine concentration could cause very significant effects on mitochondrial respiration during ADP-ATP conversion,suggesting that polyamines have regulatory effects on cellular respiration (CHAFFEE et al, 1978(CHAFFEE et al, , 1979 et al(1951). Polyamines in the brown fat were determined according to the method by perchloric acid was applied to a column of Dowex-50.Polyamines were finally eluted with 6N HCl and the eluate was evaporated.The residue was dissolved amines developed with ninhydrin reagent was measured colorimetrically.…”

mentioning

confidence: 99%