The Possible Role of Nitric Oxide Pathway in Pentylenetetrazole Preconditioning Against Seizure in Mice

Faghir‐Ghanesefat, Hedyeh; Keshavarz-Bahaghighat, Hedieh; Mohajer, Bahram; Mokhtari, Tahmineh; Bahramnejad, Erfan; Roodsari, Soheil Kazemi; Dehpour, Ahmad Reza

doi:10.1007/s12031-018-1256-2

Cited by 9 publications

(4 citation statements)

References 37 publications

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“…[61] In light of this information, the present finding indicates elevated levels of NO in brain homogenates in PTZ administered group. The present outcomes are in accordance with the study of Faghir-Ghanesefat et al [62] It was proven that sulfur-containing compounds like alpha lipoic acid are capable of preventing the overproduction of NO in epileptic brain tissues. [63] Vit U reversed the altered NO levels in PTZ group probably via its antioxidant capacity.…”

Section: Discussionsupporting

confidence: 93%

The protective effect of vitamin U on pentylenetetrazole‐induced brain damage in rats

Bayrak

Türkyılmaz

Yanardağ

2022

J Biochem & Molecular Tox

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Pentylenetetrazole (PTZ) is preferred for experimental epilepsy induction. PTZ damages brain and other organs by elevating oxidative substances. Vitamin U (Vit U) is sulfur derivative substance that proved to be an excellent antioxidant.The current study was intended to determine the protective role of Vit U on PTZ-induced brain damage. Male Sprague-Dawley rats were separated into four groups. The Control group (Group I), was given saline for 7 days intraperitoneally (i.p); Vit U (Group II) was given as 50 mg/kg/day for 7 days by gavage; PTZ was injected into animals (Group III) at a single dose of 60 mg/kg, by i.p; PTZ + Vit U group (Group IV) was administered PTZ and Vit U in same dose and time as aforementioned. After the experiment was terminated, brain tissues were taken for the preparation of homogenates. In the PTZ group, glutathione and lipid peroxidation levels, alkaline phosphatase, myeloperoxidase, xanthine oxidase, acetylcholine esterase, antioxidant enzyme activities, total oxidant status, oxidative stress index, reactive oxygen species, and nitric oxide levels were increased. However, total antioxidant capacity was decreased in the PTZ group.Vit U ameliorated these effects in the PTZ-induced brain damage. Consequently, we can suggest that Vit U protected brain tissue via its antioxidant feature against PTZ kindling epilepsy.

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Section: Discussionsupporting

confidence: 93%

The protective effect of vitamin U on pentylenetetrazole‐induced brain damage in rats

Bayrak

Türkyılmaz

Yanardağ

2022

J Biochem & Molecular Tox

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“…Although the role of NO in epilepsy has been investigated in a number of studies, there has been no consensus regarding the results [31] and the anticonvulsant [32] and pre-convulsive [33] effects of NO by seizure type, NO source, other neurological transmitters and even sex or age of animals have been reported [34]. In the present study, brain and serum levels of NO were higher in PTZ group and decreased in diazepam, sham and extract groups.…”

Section: Discussioncontrasting

confidence: 48%

Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat

et al. 2020

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Background Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study was to investigate the possible effects of CZ extract on cognitive impairment and oxidative stress induced by epilepsy in rats. Methods Rats were randomly divided into different groups. In all rats (except the sham group), kindling was performed by intraperitoneal injection of pentylenetetrazol (PTZ) at a dose of 35 mg/kg every 48 h for 14 days. Positive group received 2 mg/kg diazepam + PTZ; treatment groups received 100, 200 or 400 mg/kg CZ extract + PTZ; and one group received 0.5 mg/kg flumazenil and CZ extract + PTZ. Shuttle box and Morris Water Maze tests were used to measure memory and learning. On the last day of treatments PTZ injection was at dose of 60 mg/kg, tonic seizure threshold and mortality rate were recorded in each group. After deep anesthesia, blood was drawn from the rats’ hearts and the hippocampus of all rats was removed. Results Statistical analysis of the data showed that the CZ extract significantly increased the tonic seizure threshold and reduced the pentylenetetrazol-induced mortality and the extract dose of 400 mg/kg was selected as the most effective dose compared to the other doses. It was also found that flumazenil (a GABAA receptor antagonist) reduced the tonic seizure threshold compared to the effective dose of the extract. The results of shuttle box and Morris water maze behavioral tests showed that memory and learning decreased in the negative control group and the CZ extract treatment improved memory and learning in rats. The CZ extract also increased antioxidant capacity, decreased MDA and NO in the brain and serum of pre-treated groups in compared to the negative control group. Conclusion: It is concluded that the CZ extract has beneficial effects on learning and memory impairment in PTZ-induced epilepsy model, which has been associated with antioxidant effects in the brain or possibly exerts its effects through the GABAergic system.

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“…It has been well established that NMDRs/NO signaling modulates seizure threshold in a variety of seizure models. [35][36][37][38] Additionally, from several mechanisms that potentially contribute to the anticonvulsant effects of licofelone, the NO pathway has drawn much attention. 17,18,39 These data may indicate the potential involvement of NMDAR in the anti-seizure activity of licofelone.…”

Section: 34mentioning

confidence: 99%

Involvement of N-Methyl-D-Aspartate Receptors in the Anticonvulsive Effects of Licofelone on Pentylenetetrazole-Induced Clonic Seizure in Mice

Gholizadeh¹,

Abdolmaleki²,

Bahremand³

et al. 2021

J Epilepsy Res

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Background and Purpose: Licofelone is a dual 5-lipoxygenase/cyclooxygenase inhibitor, with well-documented anti-inflammatory and analgesic effects, which is used for treatment of osteoarthritis. Recent preclinical studies have also suggested neuroprotective and anti-oxidative properties of this drug in some neurological conditions such as seizure and epilepsy. We have recently demonstrated a role for nitric oxide (NO) signaling in the anti-epileptic activity of licofelone in two seizure models in rodents. Given the important role of N-methyl-D-aspartate receptors (NMDARs) activation in the NO production and its function in the nervous system, in the present study, we further investigated the involvement of NMDAR in the effects of licofelone (1, 3, 5, 10, and 20 mg/kg, intraperitoneal [i.p.]) in an in vivo model of seizure in mice.Methods: Clonic seizures were induced in male NMRI mice by intravenous administration of pentylenetetrazol (PTZ).Results: Acute administration of licofelone exerted anticonvulsant effects at 10 (p<0.01) and 20 mg/kg (p<0.001). A combined treatment with sub-effective doses of the selective NMDAR antagonist MK-801 (0.05 mg/kg, i.p.) and licofelone (5 mg/kg, i.p.) significantly (p<0.001) exerted an anticonvulsant effect on the PTZ-induced clonic seizures in mice. Notably, pre-treatment with the NMDAR co-agonist D-serine (30 mg/kg, i.p.) partially hindered the anticonvulsant effects of licofelone (20 mg/kg).Conclusions: Our data suggest a possible role for the NMDAR in the anticonvulsant effects of licofelone on the clonic seizures induced by PTZ in mice.

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The Possible Role of Nitric Oxide Pathway in Pentylenetetrazole Preconditioning Against Seizure in Mice

Cited by 9 publications

References 37 publications

The protective effect of vitamin U on pentylenetetrazole‐induced brain damage in rats

The protective effect of vitamin U on pentylenetetrazole‐induced brain damage in rats

Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat

Involvement of N-Methyl-D-Aspartate Receptors in the Anticonvulsive Effects of Licofelone on Pentylenetetrazole-Induced Clonic Seizure in Mice

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