The Possible Role of the Nitroso-Sulfide Signaling Pathway in the Vasomotoric Effect of Garlic Juice

Abstract

The beneficial cardiovascular effects of garlic have been reported in numerous studies. The major bioactive properties of garlic are related to organic sulfides. This study aimed to investigate whether garlic juice works exclusively due to its sulfur compounds or rather via the formation of new products of the nitroso-sulfide signaling pathway. Changes in isometric tension were measured on the precontracted aortic rings of adult normotensive Wistar rats. We evaluated NO-donor (S-nitrosoglutathione, GSNO)-induced vasorelaxation and compare it with effects of hydrogen sulfide (H2S)/GSNO and garlic/GSNO. Incubation with garlic juice increased the maximal GSNO-induced relaxation and markedly changed the character of the relaxant response. Although incubation with an H2S donor enhanced the maximal vasorelaxant response of GSNO, neither the absolute nor the relative relaxation changed over time. The mixture of GSNO with an H2S donor evoked a response similar to GSNO-induced relaxation after incubation with garlic juice. This relaxation of the H2S and GSNO mixture was soluble guanylyl cyclase (sGC) dependent, partially reduced by HNO scavenger and it was adenosine triphosphate-sensitive potassium channels (KATP) independent. In this study, we demonstrate for the first time the suggestion that H2S itself is probably not the crucial bioactive compound of garlic juice but rather potentiates the production of new signaling molecules during the GSNO-H2S interaction.

Fructose Intake Impairs the Synergistic Vasomotor Manifestation of Nitric Oxide and Hydrogen Sulfide in Rat Aorta

“…Our previous studies showed an obvious interaction between two important vasoregulatory molecules, NO and H 2 S, and their pathways and suggested the existence of a coupled nitroso–sulfide signaling pathway [ 24 , 25 ]. Herein, we confirmed that the heterogeneity of specific nitroso–sulfide vasoactive signaling exists depending on the occurrence of different pathological stages, e.g., hypertension or increased plasma glucose levels [ 26 ].…”

Arterial Hypertension and Plasma Glucose Modulate the Vasoactive Effects of Nitroso-Sulfide Coupled Signaling in Human Intrarenal Arteries

“…On the contrary, relaxation induced by the S/GSNO products was not significantly affected by the presence of cPTIO, indicating that a portion of the relaxation induced by reaction products was mediated by an alternative mechanism, which might directly activate soluble guanylate cyclase without releasing free NO. Moreover, in our recent study we recorded that the hyperpolarization of smooth muscle cells after K + -channel activation played no significant role in S/GSNO product-induced relaxation in normotensive rats [ 34 ]. In the conditions of arterial hypertension, using renal arteries isolated from spontaneously hypertensive rats (SHR) we found out that the pre-treatment with ODQ, inhibitor of soluble guanylate cyclase, significantly inhibited the vasorelaxant effect induced by the S/GSNO products so indicating the involvement of the cGMP activation in the pathway.…”

Garlic (Allium sativum L) and Its beneficial properties for health: A Review

“…Green leaves from garlic foliage (Yoshimoto et al, 2015) Reduce the blood pressure and prevent oxidative stress GPH-P (pepsin) y GPH-T (trypsin) Garlic residuals (Gao et al, 2020) Vasomotoric Organic sulfides nitroso-sulfide Garlic juice (Berenyiova et al, 2020) Inflammation, Obesity and Cardiovascular Disease Organosulfur compounds, alk(en)yl-Lcysteine sulfoxides (ACSOs), alliin, alliinase Garlic bulbs (Quesada et al, 2020) Inhibited excessive adipogenesis obesity…”