The Possible Roles of Biological Bone Constructed with Peripheral Blood Derived EPCs and BMSCs in Osteogenesis and Angiogenesis

Wu, Liming; Zhao, Xin; He, Bo; Jiang, Jie; Xie, Xing; Liu, Liu

doi:10.1155/2016/8168943

Cited by 16 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of major markers of bone differentiation i.e. Opn, Col I and Ocn are linked with the maturation of osteoblasts and bone development [46]. The degree of Ocn expression was significantly more in grad.250.top scaffolds which promoted more bone formation and mineralization in this scaffold structure.…”

Section: Discussionmentioning

confidence: 92%

In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

et al. 2020

View full text Add to dashboard Cite

Background Biomaterial-based bone tissue engineering represents a promising solution to overcome reduced residual bone volume. It has been previously demonstrated that gradient and offset architectures of three-dimensional melt electrowritten poly-caprolactone (PCL) scaffolds could successfully direct osteoblast cells differentiation toward an osteogenic lineage, resulting in mineralization. The aim of this study was therefore to evaluate the in vivo osteoconductive capacity of PCL scaffolds with these different architectures. Methods Five different calcium phosphate (CaP) coated melt electrowritten PCL pore sized scaffolds: 250 μm and 500 μm, 500 μm with 50% fibre offset (offset.50.50), tri layer gradient 250–500-750 μm (grad.250top) and 750–500-250 μm (grad.750top) were implanted into rodent critical-sized calvarial defects. Empty defects were used as a control. After 4 and 8 weeks of healing, the new bone was assessed by micro-computed tomography and immunohistochemistry. Results Significantly more newly formed bone was shown in the grad.250top scaffold 8 weeks post-implantation. Histological investigation also showed that soft tissue was replaced with newly formed bone and fully covered the grad.250top scaffold. While, the bone healing did not happen completely in the 250 μm, offset.50.50 scaffolds and blank calvaria defects following 8 weeks of implantation. Immunohistochemical analysis showed the expression of osteogenic markers was present in all scaffold groups at both time points. The mineralization marker Osteocalcin was detected with the highest intensity in the grad.250top and 500 μm scaffolds. Moreover, the expression of the endothelial markers showed that robust angiogenesis was involved in the repair process. Conclusions These results suggest that the gradient pore size structure provides superior conditions for bone regeneration.

show abstract

Section: Discussionmentioning

confidence: 92%

In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The use of MSCs/EPCs co-culture is an effective method to generate osteogenic differentiation. In our previous study, we demonstrated that EPCs enhanced MSC osteogenesis in vivo [27], and similarly report also showed that MSCs coculture with EPCs promoted significant osteogenic differentiation, when compared with in vivo MSCs monoculture [19].…”

Section: Discussionmentioning

confidence: 53%

“…Previous study showed that endothelial differentiation of MSCs only occurs in direct co-culture systems, whereas MSCs maintain their initial biological properties in in-direct culture conditions [38,39]. Abundant studies have demonstrated that factors generated by EPCs are considered crucial for the process of osteogenesis, suggests that paracrine signaling was sufficient to promote MSCs differentiation [19]. MAPKs play important roles in controlling cell proliferation, and are involved in multiple cellular pathways and functions [42,43].…”

Section: Discussionmentioning

confidence: 99%

“…In vascular biology, the role of EPCs in angiogenesis has been studied extensively, both in vitro and in vivo [18]. Because of the outstanding ability of EPCs to promote, these cells are widely used in tissue engineering approaches [19]. EPCs have also promoted bone regeneration in host bone defects, hence both EPCs and MSCs are promising candidates for bone tissue engineering, and large segmental bone defect therapies [20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Endothelial Progenitor Cells Induce Osteogenic Differentiation in Co-cultured Mesenchymal Stem Cells via the MAPK Signaling Pathway

Chu¹,

liu²,

he³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The role of bone tissue engineering is to regenerate tissue using biomaterials and stem cell based approaches. Combination of two or more cell types is one of the strategies to promote bone formation. Endothelial progenitor cells (EPCs) may enhance the osteogenic properties of mesenchymal stem cells (MSCs) and promote bone healing, this study aimed to investigate the possible mechanisms of EPCs on promoting osteogenic differentiation of MSCs.Methods: MSCs and EPCs were isolated and co-cultured in Transwell chambers, the effects of EPCs on the regulation of MSC biological properties was investigated. Real-time PCR array, qRT-PCR and western blotting were performed to explore possible signaling pathways involved in osteogenesis. The expression of osteogenesis markers and calcium nodule formation was quantified by qRT-PCR, western blotting and Alizarin Red staining. Results: Results showed that when co-cultured with EPCs, MSCs exhibited greater alkaline phosphatase (ALP) activity and increased calcium mineral deposition significantly. The mitogen-activated protein kinase (MAPK) signaling pathway was involved in this process. p38 gene expression and p38 protein phosphorylation levels showed significant up-regulation in co-cultured MSCs. Silence expression of p38 in co-cultured MSCs reduced osteogenic gene expression, protein synthesis, ALP activity and calcium nodule formation.Conclusions: These data suggest paracrine signaling from EPCs influence the biological function and promote MSCs osteogenic differentiation. Activation of the p38MAPK pathway may be the key to enhancing MSCs osteogenic differentiation via indirect interactions with EPCs.

show abstract

“…One possible source of lipid formation within the bone marrow is the disrupted balance between osteogenesis and adipogenesis of BMMSCs, and the latter assumes predominance [ 2 , 3 ]. BMMSCs, the most important MSCs in the bone morrow, are self-renewable, multipotent stem cells with the capacity to differentiate into cartilage, bone, and adipose tissue [ 4 – 6 ]. The contributing factors of the formation of osteoblasts are recognized as an inhibitor for the maturation of adipocytes and vice versa [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway

Huang

Yuan

Wei

et al. 2017

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Fat infiltration within the bone marrow is easily observed in some postmenopausal women. Those fats are mainly derived from bone marrow mesenchymal stem cells (BMMSCs). The increment of adipocytes derived from BMMSCs leads to decreased osteoblasts derived from BMMSCs, so the bidirectional differentiation of BMMSCs significantly contributes to osteoporosis. Icariin is the main extractive of Herba Epimedii which is widely used in traditional Chinese medicine. In this experiment, we investigated the effect of icariin on the bidirectional differentiation of BMMSCs through quantitative real-time PCR, immunofluorescence, western blot, and tissue sections in vitro and in vivo. We found that icariin obviously promotes osteogenesis and inhibits adipogenesis through detecting staining and gene expression. Micro-CT analysis showed that icariin treatment alleviated the loss of cancellous bone of the distal femur in ovariectomized (OVX) mice. H&E staining analysis showed that icariin-treated OVX mice obtained higher bone mass and fewer bone marrow lipid droplets than OVX mice. Western blot and immunofluorescence showed that icariin regulates the bidirectional differentiation of BMMSCs via canonical Wnt signaling. This study demonstrates that icariin exerts its antiosteoporotic effect by regulating the bidirectional differentiation of BMMSCs through the canonical Wnt signaling pathway.

show abstract

The Possible Roles of Biological Bone Constructed with Peripheral Blood Derived EPCs and BMSCs in Osteogenesis and Angiogenesis

Cited by 16 publications

References 35 publications

In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

Endothelial Progenitor Cells Induce Osteogenic Differentiation in Co-cultured Mesenchymal Stem Cells via the MAPK Signaling Pathway

Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway

Contact Info

Product

Resources

About