The post-mitotic state in neurons correlates with a stable nuclear higher-order structure

Aranda‐Anzaldo, Armando

doi:10.4161/cib.18761

Cited by 27 publications

(19 citation statements)

References 70 publications

(97 reference statements)

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“…This classical observation has stood the test of time [Woodcock and Ghosh, 2010]. In the present study, we confirm and further expand the previous evidence that the NHOS, defined by the set of structural interactions between nuclear DNA and the NM, is cell-type specific [Rivera-Mulia and Aranda-Anzaldo, 2010; Trevilla-Garc ıa and Aranda- Anzaldo, , 2012. Here, we show that the NHOS of neurons is clearly different at the large-scale from that in hepatocytes (as reflected by the significant difference in the average DNA loop size, Fig.…”

Section: Discussionsupporting

confidence: 90%

“…So far, the evidence indicates that the NHOS is cell‐type specific at the local and at the large‐scale level [Rivera‐Mulia and Aranda‐Anzaldo, ; Trevilla‐García and Aranda‐Anzaldo, , ]. Thus, it has been suggested that the NHOS is rather independent of functional constraints related to replication and/or transcription and that it is primarily established following structural and thermodynamic constraints that impinge on chromosomal DNA and the NM [Aranda‐Anzaldo, , ]. This suggestion is consistent with recent evidence that the NHOS is not conserved between similar cell types from closely related species [Silva‐Santiago et al, ].…”

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confidence: 99%

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The Set of Structural DNA-Nuclear Matrix Interactions in Neurons Is Cell-Type Specific and Rather Independent of Functional Constraints

2017

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In metazoans, nuclear DNA is organized during the interphase in negatively supercoiled loops anchored to a compartment or substructure known as the nuclear matrix. The interactions between DNA and the nuclear matrix (NM) are of higher affinity than those between DNA and chromatin proteins since the last ones do not resist the procedures for extracting the NM. The structural interactions DNA-NM constitute a set of topological relationships that define a nuclear higher order structure (NHOS) although there are further higher order levels of organization within the nucleus. So far, the evidence derived from studies with primary hepatocytes and naïve B lymphocytes indicates that the NHOS is cell-type specific at the local and at the large-scale level, and so it has been suggested that such NHOS is primary determined by structural and thermodynamic constraints. We carried out a comparative characterization of the NHOS of postmitotic cortical neurons with that of hepatocytes and naïve B lymphocytes. Our results indicate that the NHOS of neurons is completely different at the large scale and at the local level from that one observed in hepatocytes or in naïve B lymphocytes, confirming on the one hand that the set of structural DNA-NM interactions is cell-type specific and supporting, on the other hand the notion that structural constraints that impinge on chromosomal DNA and the NM are more important for determining this NHOS than functional constraints related to replication and/or transcription. J. Cell. Biochem. 118: 2151-2160, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%

The Set of Structural DNA-Nuclear Matrix Interactions in Neurons Is Cell-Type Specific and Rather Independent of Functional Constraints

2017

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“…EDTP/MTMR14 has two main functions: negative regulation of autophagy and disease-causing effect if deficient in the muscles [1,[11][12][13][14][15]. Drosophila EDTP has two peaks of expression, one Motoneurons are highly specialized and terminally differentiated with reduced function of global genome repair [32,33]. More importantly, neuronal cells utilize autophagy as an essential process for normal turnover of cytoplasmic contents [34].…”

Section: Discussionmentioning

confidence: 99%

Drosophilaegg-derived tyrosine phosphatase (EDTP): a novel target for improved survivorship to prolonged anoxia and cellular protein aggregates

Xiao

Qiu

et al. 2018

Preprint

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Drosophila egg-derived tyrosine phosphatase (EDTP), a lipid phosphatase that removes 3position phosphate at the inositol ring, has dual functions in the oogenesis and the muscle performance during adult stages. A mammalian homologous gene MTMR14, which encodes the myotubularin-related protein 14, negatively regulates autophagy. Mutation of EDTP/MTMR14, however, causes at least three deleterious consequences: (1) lethality in the early embryogenesis in Drosophila; (2) "jumpy" phenotype with apparently impaired motor functions; and (3) association with a rare genetic disorder called centronuclear myopathy. Here we show that flies carrying a heterozygous EDTP mutation had increased survivorship to prolonged anoxia; tissuespecific downregulation of EDTP in non-muscle tissues, particularly motoneurons, extended the lifespan; and tissue-specific downregulation of EDTP in motoneurons improved the survivorship to beta-amyloid peptides (Aβ42) and polyglutamine (polyQ) protein aggregates. MTMR14 expression was evident in the hippocampus and cortex in C57BL/6J and APP/PS1 mice.Compared with C57BL/6J mice, APP/PS1 mice had reduced MTMR14 in the cortex but not in the hippocampus. Hippocampal expression of MTMR14 was increased and plateaued at 9-17 months compared with 2-6 months in C57BL/6J mice. Aβ42 treatment increased the expression of MTMR14 in the primarily cultured hippocampal neurons of Sprague/Dawley rats and mouse Neuro2a neuroblasts. We demonstrated a novel approach of tissue-specific manipulation of the disease-associated gene EDTP/MTMR14 for lifespan extension and the improvement of survivorship to cellular protein aggregates.

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“…4). Because heavy metal toxicity in PSP preferentially affects neurons 29 , which are non-dividing post-mitotic cells 30 , we compared the sensitivity of SH-SY5Y cells to heavy metals in both non-differentiated and neuron-like differentiated states.…”

Section: Chromium and Nickel Induced Cell Death In Cycling And Terminmentioning

confidence: 99%

Heavy metals contaminating the environment of a progressive supranuclear palsy cluster induce tau accumulation and cell death in cultured neurons

Alquézar

Felix

McCandlish

et al. 2020

Sci Rep

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The post-mitotic state in neurons correlates with a stable nuclear higher-order structure

Cited by 27 publications

References 70 publications

The Set of Structural DNA-Nuclear Matrix Interactions in Neurons Is Cell-Type Specific and Rather Independent of Functional Constraints

The Set of Structural DNA-Nuclear Matrix Interactions in Neurons Is Cell-Type Specific and Rather Independent of Functional Constraints

Drosophilaegg-derived tyrosine phosphatase (EDTP): a novel target for improved survivorship to prolonged anoxia and cellular protein aggregates

Heavy metals contaminating the environment of a progressive supranuclear palsy cluster induce tau accumulation and cell death in cultured neurons

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