The Posttranscriptional Mechanism in Salvia miltiorrhiza Bunge Leaves in Response to Drought Stress Using Phosphoproteomics

Abstract

Drought stress is a major constraint to the quality and production of Salvia miltiorrhiza Bunge (Danshen). This study aimed to investigate the posttranslational molecular mechanisms in S. miltiorrhiza leaves in response to drought stress using quantitative phosphoproteomics analysis. S. miltiorrhiza plants were stressed by withholding water for two (moderate drought stress) and four weeks (high drought stress). Leaf samples were prepared with tandem mass tag labeling. Liquid chromatography-tandem mass spectrometry was performed for the quantitative phosphoproteomics. Bioinformatics methods were used to identify the phosphosites and phosphoproteins that had significantly changed phosphorylation levels upon drought stresses. A total of 119 common phosphoproteins were significantly changed by both high and moderate drought stresses. The phosphorylation levels of proteins related to protein processing, photosynthesis, RNA binding, and splicing were significantly changed upon high drought, not moderate drought. Additionally, we identified that the Ser phosphorylation levels of most proteins related to terpene metabolism and RNA splicing were regulated by drought stresses. The Ser and Thr phosphorylation levels of energy metabolism proteins (including FBA2/8, PPC4, and PPCC) and heat shock proteins (including HSP70 and HSP90) were upregulated by drought stresses. Our study showed the posttranscriptional mechanisms in S. miltiorrhiza leaves in response to drought stress.

Unraveling the adaptive mechanisms of Veronica nakaiana in response to drought stress: a transcriptome-based study

“…In our study, six bZIP TFs were upregulated in DS plants (Table S3). Furthermore, seven bHLH family genes, including the three SPATULA genes mentioned above, were upregulated, suggesting their potential involvement in the drought stress mechanism, as reported in other plant species [70][71][72][73] . Among the other upregulated TFs, one belonged to the NAC family, six to WRKY family, and seven to the GATA family (Table S3).…”

Rapid diversification of grey mangroves (Avicennia marina) driven by geographic isolation and extreme environmental conditions in the Arabian Peninsula

“…It has been proposed that SQE genes can play a role in the regulation of reactive oxygen species, stomatal responses and drought tolerance, likely mediated by the involvement of sterols in the localization of NADPH oxidases (Posé et al., 2009). The 4‐coumarate‐CoA ligase‐like 7 (4CLL7) gene encodes an enzyme related to terpenoid metabolism potentially involved in response to drought stress, as it has been reported for red sage (Madritsch et al., 2019; Zhang et al., 2022). The abscisic acid‐insensitive 5‐like (AI5L5) gene is similar in function to ABI5, a well‐known transcription factor involved in the response to the plant hormone ABA pathway.…”

“…gene encodes an enzyme related to terpenoid metabolism potentially involved in response to drought stress, as it has been reported for red sage (Madritsch et al, 2019;Zhang et al, 2022). The abscisic acid-insensitive 5-like (AI5L5) gene is similar in function to ABI5, a well-known transcription factor involved in the response to the plant hormone ABA pathway.…”

Rapid diversification of gray mangroves (Avicennia marina) driven by geographic isolation and extreme environmental conditions in the Arabian Peninsula

“…It has been proposed that SQE genes can play a role in the regulation of reactive oxygen species, stomatal responses and drought tolerance, likely mediated by the involvement of sterols in the localization of NADPH oxidases (Posé et al, 2009). The 4-coumarate-CoA ligase-like 7 (4CLL7) gene encodes an enzyme related to terpenoid metabolism potentially involved in response to drought stress, as it has been reported for red sage (Madritsch et al, 2019;Zhang, Li, Su, Song, & Wang, 2022). The abscisic acid-insensitive 5-like (AI5L5) gene is similar in function to ABI5, a well-known transcription factor involved in the response to the plant hormone ABA pathway.…”