The Potency of Aluminum Hydroxide Nanoparticles  for Dengue Subunit Vaccine Adjuvant

Pambudi, Sabar; Mardliyati, Etik; Rahmani, Silmi; Setyawati, Damai Ria; Widayanti, Tika; Gill, Angelina; Sulfianti, Asri; LESTARI, WHINIE

doi:10.5454/mi.12.3.5

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…The cell viability assay was carried out to evaluate the cytotoxicity of the nanoparticle chitosan encapsulated with liposome as an adjuvant candidate on RAW 264.7 cells (kindly gifted by Dr. Paik of KRIBB, South Korea) using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay, as previously described by other groups [18][19][20]. Briefly, the cells were seeded on a 96-well plate at 50,000 cells per well in triplicate, and grown in the culture medium of Dulbecco's Modified Eagle Medium-High Glucose (DMEM-HG) supplemented with 10% Fetal Bovine Serum (FBS) and 100 units/mL penicillin-streptomycin in a humidified incubator with 5% CO2 at 37 o C for 20-24 hours.…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…The nitric oxide (NO) release assay was determined by measuring the quantity of nitrite in the supernatant by the Griess reaction, as previously reported [18,21,22]. Briefly, the cells were seeded on a 96-well plate at 120,000 cells per well in triplicate, and grown in the culture medium of DMEM-HG supplemented with 10% Fetal Bovine Serum (FBS), and 100 units/well penicillin-streptomycin, in a humidified incubator with 5% CO2 at 37°C for 24 hours to reach 90-95%.…”

Section: Nitric Oxide Release Assaysmentioning

confidence: 99%

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Preparation, characterization, and in-vitro evaluation of liposome-coated chitosan nanoparticle as immune adjuvant candidate in RAW 264.7 cells

Tarwadi,

Adliani,

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Chitosan has been explored for various biological applications due to its biodegradability and biocompatibility. In this research, we prepared, characterized, and evaluated liposome-coated chitosan nanoparticles for immune adjuvant against bovine serum albumin (BSA) as a vaccine model. The liposome was formulated using DPPC, cholesterol, and DSPE-PEG with a molar ratio of 9.5:9.5:1. The BSA-chitosan nanoparticle was generated using the ionic gelation method prepared at pH 2.8, 5.5, and 8.7 before it was encapsulated with the liposome. The adjuvant was evaluated for its degree of encapsulation efficiency by Bradford reagent, and the particle size was determined using Zetasizer. Cell viability and nitric oxide release assays were carried out in RAW 264.7 cells. It was found that encapsulation efficiency was in the range of 72% to 85%, and the smallest particle size of the adjuvant-BSA was approximately ∼ 200 nm with a polydispersity index of ∼ 0.04. MTT assays have shown that cell viability was around 80%-90% compared to the control and capable of inducing nitric oxide release (p<0.05). Further exploration should be carried out in order to further discover cytokine release, humoral, and cellular immune responses.

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Section: Cell Viability Assaymentioning

confidence: 99%

Section: Nitric Oxide Release Assaysmentioning

confidence: 99%

Preparation, characterization, and in-vitro evaluation of liposome-coated chitosan nanoparticle as immune adjuvant candidate in RAW 264.7 cells

Tarwadi,

Adliani,

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Based on this concept, tremendous efforts to amplify the performance of the adjuvant have been made by modifying its particle size into nano-sized adjuvant [3]. From our previous report, a stabilized AH nanoparticle (AHNp) with the average size of ~200 nm exhibited stronger immunopotentiation activity of purified DENV3 pre-Membrane Envelope (prM-E) recombinant protein than those of micro-sized counterpart (AHMp) without altering its intrinsic toxicity profile [4] [5]. Besides the aforementioned parameters, the binding of protein and adjuvant has been intensively studied to establish the optimum condition of adjuvant-antigen interaction which is contribute directly to the immuneenhancing effect.…”

Section: Introductionmentioning

confidence: 99%

Effect of Sterilization on Protein Adsorption of Micro- and Nano-sized Aluminum Hydroxide Adjuvant

Setyawati¹,

Muttaqien²,

Ramadhan³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Aluminum hydroxide (AH) is one of the aluminum-based adjuvants that forms needle-like crystalline structure with the average particle size of 1-20 µm. Due to their favorable safety profile, AH adjuvant has been used widely in various human vaccine in diphtheria, tetanus, pertussis, hepatitis, and papilloma virus vaccines. The antigen-adjuvant interaction is strongly governed by the adjuvant's physicochemical characters, such as shape, dimension and surface charge. Based on this concept, tremendous efforts to amplify the performance of the adjuvant have been made by modifying its particle size into nano-sized adjuvant. Also, during vaccine formulation, several physical processes may take place and potentially affect the antigen-adjuvant interaction, such as dilution, salt and aging exposure, pH change, heat, pressure and shear forces stress. Therefore, in this study we have examined the effect of sterilization and pH of AH nanoparticle (AHNp) with the average size of ~160 nm on its physical characterization and adjuvant-antigen interaction. The obtained results were compared with those from AH microparticle (AHMp) and commercial AH adjuvant (AHC).AHMp and AHNp were prepared by using common precipitation method through the addition of alkali to the solution of aluminum chloride. The aluminum content of both AH suspension is 12.1 mg/mL. The average particle size of AHMp and AHNp by static light scattering are 4.21 µm and 0.16 µm, respectively. For evaluating the effect of autoclave on AH properties, all AH samples on glass container were autoclaved at 121 °C for 30 dan 60 min. The result shows that all samples did not give significant physical character change after autoclave. All measured parameters including the solid content of AH suspension, the acquired mass after freezing dry, pH value, and visual appearance remained the same as those before autoclave. The X-ray diffractogram of AHMp and AHNp revealed that the diffraction bands were sharper after autoclaving.Following autoclaving, all fraction of (bovine serum albumin) BSA added into the adjuvant could be completely adsorbed by AHNp, AHMp, and AHC samples, as high as its pre-autoclaved condition, achieving maximum adsorption capacity. Such phenomena could be obtained even when volume ratio of adjuvant/BSA multiplied 20-times. It indicates that the binding efficiency BSA to all adjuvant

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Nanoscale alum-based adjuvants: Current status and future prospects

Mardliyati,

Hawa Syaifie,

El Muttaqien

et al. 2024

Materials Today: Proceedings

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The Potency of Aluminum Hydroxide Nanoparticles for Dengue Subunit Vaccine Adjuvant

Cited by 3 publications

References 23 publications

Preparation, characterization, and in-vitro evaluation of liposome-coated chitosan nanoparticle as immune adjuvant candidate in RAW 264.7 cells

Preparation, characterization, and in-vitro evaluation of liposome-coated chitosan nanoparticle as immune adjuvant candidate in RAW 264.7 cells

Effect of Sterilization on Protein Adsorption of Micro- and Nano-sized Aluminum Hydroxide Adjuvant

Nanoscale alum-based adjuvants: Current status and future prospects

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