The potent therapeutic effect of novel cyanobacterial isolates against oxidative stress damage in redox rats

Abstract

Aims: Cyanobacteria are immense sources of several pharmacological active compounds such as flavonoids and carotenoids with anti-inflammatory and antioxidant activity. The potential therapeutic effect of two novel cyanobacterial isolates, Cronbergia siamensis (KY296358.1) and Sphaerospermopsis aphanizomenoides (KU212886.1), against hydrogen peroxide (H 2 O 2 )induced oxidative stress damage in the rat model was determined in this study. Methods and Results: In vitro antioxidant activity of the two studied isolates was evaluated by radical scavenging assay and ferric reducing power. The possible prophylactic activity of S. aphanizomenoides (KU212886.1) against H 2 O 2 -induced oxidative stress in the rat model was assessed in vivo. Serum alanine transaminase and aspartate transaminase were measured for the liver functions in redox rats. Liver malondialdehyde (MDA), glutathione, oxidized glutathione, nitric oxide, superoxide dismutase (SOD) and catalase (CAT) were assessed as oxidative stress markers. The effect of S. aphanizomenoides on the transcripts level of superoxide dismutase (Mn-SOD) and catalase (CAT) genes in the rat's liver tissues was measured using qRT-PCR. Oral administration of S. aphanizomenoides extract in low and high doses (100, 200 mg kg À1 b.w) resulted in significant improvement in biochemical parameters of liver functions and oxidative stress markers. Also, the endogenous antioxidant defence enzymes and the expression of their related genes (Mn/SOD, CAT) were upregulated. Immunohistochemistry of Caspase-3, an apoptotic marker, showed potent amelioration in the liver tissues. Conclusions: The novel isolate S. aphanizomenoides proved in vitro and in vivo antioxidant activity against redox rat model. Significance and Impact of the Study: This isolate provides a new source of pharmacological compounds with great importance in pharmacological and medical fields.The phenolic compound was measured by Agilent HPLC 1260 Infinity II, system; the system consisted of autosampler, quaternary pumps, UV detector, an interface, reversed-phase column alternative for LiChrospher 100 RP-c18, 250 mm 9 4Á0 mm I.D., 5 lm particle size. The

Prophylactic Evidence of MSCs-Derived Exosomes in Doxorubicin/Trastuzumab-Induced Cardiotoxicity: Beyond Mechanistic Target of NRG-1/Erb Signaling Pathway

“…This was evident by decreased NCX1 immunostaining in group II cardiac tissue compared to controls. Notably, perturbations in the expression and function of NCX1 have been demonstrated in various clinical and experimental settings of cardiac dysfunction [ 43 ], including DOX-induced cardiac toxicity [ 44 , 45 ]. Additionally, Ujihara et al have reported maintaining NCX1 activity as a promising strategy for protection against heart failure [ 46 ].…”

Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

“…To our knowledge, there are no reported data on the protective effect of cyanobacterial extracts against H 2 O 2 -induced oxidative stress in human cell lines, as has already been described for other natural compounds such as ferrulic acid, a natural phenolic present in plants and fruits [ 36 ] and Phyllanthus phillyreifolius , an endogenous pant used in traditional medicine in Réunion island [ 37 ]. However, a study by Badr et al [ 38 ] revealed a decrease in oxidative stress markers and an increase in endogenous antioxidant defenses in rats under H 2 O 2 -induced oxidative stress, when orally consuming an extract of the cyanobacterial strain Sphaerospermopsis aphanizomenoides . In the present study, the extracts of Nostoc sp.…”

Comparative evaluation of growth performance, carcass characteristics and timed series gene expression profile of GH and IGF‐1 in two Egyptian indigenous chicken breeds versus Rhode Island Red

“…(2002), while the endogenous antioxidant enzyme (SOD) was analysed by spectrophotometer according to Badr et al. (2019). Protein carbonyl assay was performed based on the method of Patsoukis et al.…”