The Potential Cardioprotective Effects of Hydrogen in Irradiated Mice

Qian, Liren; Cao, Fei; Cui, Jianguo; Wang, Yicun; Huang, Yuecheng; Chuai, Yunhai; Zaho, Luqian; Jiang, Hao; Cai, Jianming

doi:10.1269/jrr.10093

Cited by 80 publications

(72 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, many studies have reported the use of hydrogen therapy in different diseases, including those involving the nervous, digestive, cardiovascular and respiratory systems. The protective effects of hydrogen have also been confirmed in different animal models, including the ability to limit the infarct volume of the brain, heart, intestines and kidneys by reducing ischemia-reperfusion injury without altering hemodynamic parameters and providing protection against multiple organ damage elicited by generalized inflammation34567891011121314151617. These studies suggest that hydrogen plays a beneficial role in clinical applications in various organs.…”

mentioning

confidence: 77%

Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes

Liu

Kurokawa²,

Fujino

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Hydrogen exerts beneficial effects in disease animal models of ischemia-reperfusion injury as well as inflammatory and neurological disease. Additionally, molecular hydrogen is useful for various novel medical and therapeutic applications in the clinical setting. In the present study, the hydrogen concentration in rat blood and tissue was estimated. Wistar rats were orally administered hydrogen super-rich water (HSRW), intraperitoneal and intravenous administration of hydrogen super-rich saline (HSRS), and inhalation of hydrogen gas. A new method for determining the hydrogen concentration was then applied using high-quality sensor gas chromatography, after which the specimen was prepared via tissue homogenization in airtight tubes. This method allowed for the sensitive and stable determination of the hydrogen concentration. The hydrogen concentration reached a peak at 5 minutes after oral and intraperitoneal administration, compared to 1 minute after intravenous administration. Following inhalation of hydrogen gas, the hydrogen concentration was found to be significantly increased at 30 minutes and maintained the same level thereafter. These results demonstrate that accurately determining the hydrogen concentration in rat blood and organ tissue is very useful and important for the application of various novel medical and therapeutic therapies using molecular hydrogen.

show abstract

mentioning

confidence: 77%

Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes

Liu

Kurokawa²,

Fujino

et al. 2014

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This study suggested that serial measurements of cardiac biomarkers might facilitate the management of patients undergoing radiation therapy and might help to define subgroups at high risk of developing heart failure. Also Qian et al (2010) found that hydrogen had a cardioprotective effect against radiation-induced injury.…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of peroxisome proliferator-activated receptor gamma in radiation-induced heart injury in rats

Gao

Zeng

2011

Radiat Environ Biophys

View full text Add to dashboard Cite

To explore the expression level and the role of peroxisome proliferator-activated receptor gamma (PPAR-γ) in radiation-induced heart injury in a rat model, thirty-two Sprague-Dawley rats were divided into three groups (the control group, the 15-Gy irradiation group and the 18-Gy irradiation group). Experimental animals were exposed to radiation generated by a linear accelerator at the chest and killed after 3 months. Heart tissues from these animals were removed for Masson staining, PPAR-γ immunohistochemical staining, Western blot analysis and real-time polymerase chain reaction assay (RT-PCR). In addition, the protein expression of matrix metalloprotein-1 (MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor type beta1 (TGF-β1), all of which are associated with fibrosis, was measured. Masson staining revealed significant myocardial fibrosis, degeneration and necrosis in rats exposed to radiation. The results of immunohistochemical staining and Western blot analysis showed that PPAR-γ protein expression in hearts of the irradiation groups was significantly higher than in the control group, especially in myocardium and vascular endothelial (p < 0.05). RT-PCR results also showed a parallel increase in PPAR-γ mRNA expression in the heart of the irradiation groups compared with the control group (p < 0.05). The expression of MMP-1 protein was not significantly different in three groups (p > 0.05). The expression of TIMP-1 and TGF-β1 proteins was, however, higher in two irradiation groups than in the control group (p < 0.05). These data demonstrate that PPAR-γ expression is up-regulated on both mRNA and protein levels in heart injured by radiation. PPAR-γ may play an important role in radiation-induced heart injury.

show abstract

“…For example, Monceau et al delivered a dose of 16 Gy to the thorax of C57BL/6 mice and witnessed radiation-induced cardiac hypertrophy 15 weeks following irradiation (19); however, a significant decrease in ejection fraction did not occur until 30 weeks. Qian et al (34) evaluated short-term effects of 7 Gy delivered to the heart, and found that a mild myocyte degeneration was not witnessed until 100 days following treatment. A study Fig.…”

Section: Discussionmentioning

confidence: 99%

X-ray dose delivered during a longitudinal micro-CT study has no adverse effect on cardiac and pulmonary tissue in C57BL/6 mice

Detombe

Dunmore‐Buyze²,

Petrov³

et al. 2013

Acta Radiol

View full text Add to dashboard Cite

This study demonstrated that a 5 Gy X-ray dose accumulated over 6 weeks during a longitudinal micro-CT study had no significant effects on the pulmonary and myocardial tissue of C57BL/6 mice. As a result, the many advantages of micro-CT imaging, including rapid acquisition of high-resolution, isotropic images in free-breathing mice, can be taken advantage of in longitudinal studies without concern for negative dose-related effects.

show abstract

The Potential Cardioprotective Effects of Hydrogen in Irradiated Mice

Cited by 80 publications

References 30 publications

Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes

Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes

Up-regulation of peroxisome proliferator-activated receptor gamma in radiation-induced heart injury in rats

X-ray dose delivered during a longitudinal micro-CT study has no adverse effect on cardiac and pulmonary tissue in C57BL/6 mice

Contact Info

Product

Resources

About