The potential effects of Indigofera coerulea extract on THP-1 human cell line

Al-Nasser, Muneerah M.; Al‐Dosari, Mohammed S.; Parvez, Mohammad K.; Al-Anazi, Mashael R.; Alkahtane, Abdullah A.; Alothaid, Hani; Alahmari, Abeer; Alarifi, Saud; Albasher, Gadah; Almeer, Rafa; Alqahtani, Mohamed D.; AL–Johani, Norah S.; Alhoshani, Norah M.; Alkeraishan, Nora; Alhenaky, Alhanof; Alkahtani, Saad; Al‐Qahtani, Ahmed

doi:10.1016/j.jksus.2021.101446

Cited by 5 publications

(3 citation statements)

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“…Providing that the mechanism of action of miRNA usually links to mRNA inhibition (target repression), plant-derived miR482f and miR482c-5p could eventually downregulate inflammation-related genes. To eventually validate this hypothesis, we used differentiated human THP-1 macrophages, an in vitro cell model widely used in the study of the antioxidant and anti-inflammatory bioactive properties of chemical compounds and derived food products ( 46 – 48 ).…”

Section: Discussionmentioning

confidence: 99%

miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages

Díez-Sainz,

Lorente-Cebrián,

Aranaz

et al. 2023

Front. Nutr.

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BackgroundEdible plants can exert anti-inflammatory activities in humans, being potentially useful in the treatment of inflammatory diseases. Plant-derived microRNAs have emerged as cross-kingdom gene expression regulators and could act as bioactive molecules involved in the beneficial effects of some edible plants. We investigated the role of edible plant-derived microRNAs in the modulation of pro-inflammatory human genes.MethodsMicroRNAs from plant-derived foods were identified by next-generation sequencing. MicroRNAs with inflammatory putative targets were selected, after performing in silico analyses. The expression of candidate plant-derived miRNAs was analyzed by qPCR in edible plant-derived foods and their effects were evaluated in THP-1 monocytes differentiated to macrophages. The bioavailability of candidate plant miRNAs in humans was evaluated in feces and serum samples by qPCR.ResultsmiR482f and miR482c-5p are present in several edible plant-derived foods, such as fruits, vegetables, and cooked legumes and cereals, and fats and oils. Transfections with miR482f and miR482c-5p mimics decreased the gene expression of CLEC7A and NFAM1, and TRL6, respectively, in human THP-1 monocytes differentiated to macrophages, which had an impact on gene expression profile of inflammatory biomarkers. Both microRNAs (miR482f and miR482c-5p) resisted degradation during digestion and were detected in human feces, although not in serum.ConclusionOur findings suggest that miR482f and miR482c-5p can promote an anti-inflammatory gene expression profile in human macrophages in vitro and their bioavailability in humans can be achieved through diet, but eventually restricted at the gut level.

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Section: Discussionmentioning

confidence: 99%

miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages

Díez-Sainz,

Lorente-Cebrián,

Aranaz

et al. 2023

Front. Nutr.

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“… 1 , 2 Research on inflammation signaling pathways has accelerated the exploration of targets for drug discovery. 3 In case of dangerous stimuli, macrophages take the major role in the inflammatory response. In vitro cancer, monocyte–macrophage differentiation, and macrophage-related physiological process studies are usually conducted with THP-1 cells.…”

Section: Introductionmentioning

confidence: 99%

“…Inflammation is the complex biological response of body tissues to harmful infections with pathogens, damaged cells, or irritants, and inflammation is a protective reaction involving immune cells, blood vessels, and molecular mediators. Arteriosclerosis, obesity, liver diseases, autoimmune diseases, Alzheimer’s disease, and cancer are the results of excessive or chronic inflammatory responses. , Research on inflammation signaling pathways has accelerated the exploration of targets for drug discovery . In case of dangerous stimuli, macrophages take the major role in the inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Link between Inflammasome and Apoptosis through the Response of THP-1 Cells against Drugs Using Droplet-Based Microfluidics

et al. 2022

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Droplet-based microfluidic devices are used to investigate monocytic THP-1 cells in response to drug administration. Consistent and reproducible droplets are created, each of which acts as a bioreactor to carry out single cell experiments with minimized contamination and live cell tracking under an inverted fluorescence microscope for more than 2 days. Here, the effects of three different drugs (temsirolimus, rifabutin, and BAY 11-7082) on THP-1 are examined and the results are analyzed in the context of the inflammasome and apoptosis relationship. The ASC adaptor gene tagged with GFP is monitored as the inflammasome reporter. Thus, a systematic way is presented for deciphering cell-to-cell heterogeneity, which is an important issue in cancer treatment. The drug temsirolimus, which has effects of disrupting the mTOR pathway and triggering apoptosis in tumor cells, causes THP-1 cells to express ASC and to be involved in apoptosis. Treatment with rifabutin, which inhibits proliferation and initiates apoptosis in cells, affects ASC expression by first increasing and then decreasing it. CASP-3, which has a role in apoptosis and is directly related to ASC, has an increasing level in inflammasome conditioning. Thus, the cell under the effect of rifabutin might be faced with programmed cell death faster. The drug BAY 11-7082, which is responsible for NFκB inhibition, shows similar results to temsirolimus with more than 60% of cells having high fluorescence intensity (ASC expression). The microfluidic platform presented here offers strong potential for studying newly developed small-molecule inhibitors for personalized/precision medicine.

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Phytochemical profiling, anti-oxidant, antimicrobial and cytotoxic evaluation of Bergera koenigii seed extracts against Leukemic cancer

Govindswamy

2024

Preprint

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Traditional herbs have always been pioneers in the development of new therapeutics. According to Ayurveda, India has always been a hub of many herbs and shrubs that possess numerous polyphenols and flavonoids with promising anticancer, antioxidant, and antibacterial properties. The present study aimed to elucidate the anticancer activity of various methanol, ethanol and petroleum ether extracts of Bergera koenigii seeds against THP-1 cells. In addition to phytochemical analysis, total phenol and flavonoid content (TPC, TFC) and antioxidant assays were also performed to estimate the potential of the Bergera koenigii seeds. Among these, the methanolic extract of Bergera koenigii seeds inhibited leukemic THP-1 cells due to the presence of bioactive compounds such as hexadecanoic acid, octadecadienoic acid, octadecatrienoic acid, tricosanoic acid and γ-sitosterol, as revealed by GC‒MS analysis. According to the radical scavenging capacity, both hexadecanoic acid and octadecadienoic acid exhibited the highest anticancer activity, with IC50 values of 15±8.37 and 15±0.23 µg/ml, respectively. Additionally, the methanolic extract had a TPC of 959.97 mg/GAE and a TFC of 1443.20 mg/QE, and the antioxidant activity had an IC50 value of 369.09, Antibacterial activity with 10 ± 0.5 mmEscherichia coli, 8 ± 0.82 mm for Pseudomonas aeruginosa and15 ± 1.7 mm for Staphylococcus aureus. Thus, the above results indicate that the methanolic extracts of Bergera koenigii seeds are more promising drugs against leukemic THP-1 cell lines than are the chloroform and pet ether extracts.

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The potential effects of Indigofera coerulea extract on THP-1 human cell line

Cited by 5 publications

References 34 publications

miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages

miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages

Understanding the Link between Inflammasome and Apoptosis through the Response of THP-1 Cells against Drugs Using Droplet-Based Microfluidics

Phytochemical profiling, anti-oxidant, antimicrobial and cytotoxic evaluation of Bergera koenigii seed extracts against Leukemic cancer

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