The Potential Mechanism of ZFX Involvement in the Cell Growth

Abstract

Background: The zinc-finger X linked (ZFX) gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim of this study is to define ZFX protein-protein interactions and the role of ZFX in cell growth. Materials and Methods: The PIPs output includes three interacting proteins with ZFX: eukaryotic translation initiation factor 3 subunit I (EIF3I), eukaryotic translation initiation factor 3 subunit G (EIF3G) and protein nuclear pore and COPII coat complex component homolog isoform 3 (SEC13L1). Results: As a cargo and transmembrane protein interacting with Sec13, eIF3I and eIF3G, ZFX mediates cargo sorting in COPII vesicles at ER exit sites. While traveling to cis-Golgi, eIF3I is phosphorylated by the mechanistic target of rapamycin (mTOR). Proteins transport by COPI vesicles to the nucleusouter site layer containing SEC13 via the contribution of microtubules. EIF3G and eIF3I interact with coatomer protein complex subunit beta 2 (COPB2) that helps to enclose ZFX in COPI vesicle. ZFX and eIF3G enter nucleolus where activation of transcription from pre rDNA genes occurs. Conclusion: We proposed a model in which ZFX is involved in cell growth by promoting the transcription of rDNA genes.

RICTOR Drives ZFX-mediated Ganglioside Biosynthesis to Promote Breast Cancer Progression

“…ZFX is a highly conserved Zinc figure protein and oncogenic transcription factor residing on the X chromosome, and is overexpressed in many cancers (33)(34)(35). To functionally validate the role of ZFX in UGCG regulation, we used ZFX-overexpressed MCF-7 cells (MCF-7_ZFX OE ), and only vector overexpressed (MCF-7_VECT OE ) cells as control, along with siRNA-mediated ZFX-silenced MCF-7 cells (MCF-7_ZFX SL ) with scrambled siRNA-transfected cells (MCF-7_SCRAM SL ) as control.…”