The potential of chiroptical and vibrational spectroscopy of blood plasma for the discrimination between colon cancer patients and the control group

Abstract

Colorectal cancer is one of the most abundant causes of cancer deaths in the world. At an early stage, the established clinical procedures have low reliability and sensitivity. Therefore, we tested a novel approach based on chiroptical methods such as electronic circular dichroism (ECD) and Raman optical activity (ROA). These methods are suitable for detecting slight changes in the 3D structure of chiral biomolecules, some of which may be caused by pathological processes occurring during cancer growth. Fifty-five blood plasma samples were analyzed using the combination of ECD and ROA supplemented by conventional Raman and FT-IR spectroscopy. All obtained spectra were evaluated together by linear discriminant analysis. The accuracy of sample discrimination reached 100% and the subsequent leave-one-out cross-validation resulted in 93% sensitivity and 81% specificity. The achieved results indicate that chiroptical methods supplemented by Raman and FT-IR spectroscopy might be new supporting and minimally invasive tools in the clinical diagnosis of colon cancer.

Atomic force microscopy and Raman spectra profile of blood components associated with exposure to cigarette smoking

“…The most intense bands observed were 1005 cm À1 , 1156 cm À1 and 1517 cm À1 corresponding to carotenoid assignments, due to the enhance resonance of this structures induced by the used laser in our experiment. 69,70 We observed a signicant increase in peak 1156 cm À1 associated to carotenoid and proteins vibrations in smokers >10. It is known that carotenoids are antioxidant obtained from diet and that they are stored in lipid tissue.…”

Chiroptical spectroscopy and metabolomics for blood‐based sensing of pancreatic cancer

“…Recently, attention has been focused on PC at the genetic level, with the inspection of micro‐RNAs showing a promising diagnostic potential, as well as suggesting that a reliable biomarker panel might emerge from biofluids if innovative approaches are applied . Because biomolecules in human biofluids are already routinely used to determine the biomarkers of several diseases, blood plasma seems to be a useful probe also for the screening of PC.…”

“…In our previous studies, we developed a methodology for the smooth spectroscopic analysis of human blood plasma that combines both conventional and chiroptical forms of spectroscopy . We have also successfully applied our methodology to the identification of differences between the blood plasma of healthy subjects and that of patients suffering from type 1 diabetes and colon cancer …”

Electronic circular dichroism for the detection of microalbuminuria

“…However, the vast majority of chiroptical studies have been devoted to single molecules or simple systems, which are not burdened by the complexity of real biofluids. 19,20 However, the potential of chiroptical spectroscopy for the direct monitoring of urinary albumin levels in diabetic patients remains untapped. [9][10][11][12][13][14][15][16][17] A number of them have successfully used chiroptical spectroscopy of blood plasma, serum, or urine for [This article is part of the Special Issue: Proceedings 16th International Conference on Chiroptical Spectroscopy, Rennes France 2017.…”

“…pharmacokinetic studies, 9,10,12,13 cholesterol quantification, 18 and even the determination of disease-specific spectral signatures. 19,20 However, the potential of chiroptical spectroscopy for the direct monitoring of urinary albumin levels in diabetic patients remains untapped.…”