The potential of copper-resistant bacteria Acinetobacter sp. strain CN5 in decolorizing dyes

Irawati, Wahyu; Pinontoan, Reinhard; MOURETTA, BELLEN; Yuwono, Triwibowo

doi:10.13057/biodiv/d230212

Cited by 5 publications

(16 citation statements)

References 34 publications

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“…A decrease in absorbance at a wavelength of 663 nm indicates decolorization of methylene blue while a decrease in absorbance at a wavelength of 597 nm indicates decolorization of the Reactive Black. The percentage decolorization value was measured using the formula below (Irawati et al 2022):…”

Section: Dye-resistance and Decolorization Assaymentioning

confidence: 99%

Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes

Irawati

TIMOTHY²,

SOENTORO³

et al. 2023

Biodiversitas

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Abstract. Irawati W, Timothy M, Soentoro SE, Pinontoan R, Yuwono T, Lindarto V. 2022. Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes. Biodiversitas 23: 6661-6668. Study on the characterization of multiple copper and dyes resistance in bacteria has so far been quite limited. It is, therefore, of interest to isolate and characterize such bacteria. The study was aimed to isolate bacteria from the Kapuas River in Indonesia and measuring copper resistance as well as dye-resistance and decolorization abilities. Copper resistance was determined by measuring the minimum inhibitory concentration (MIC) of copper, while dye-resistance was measured by observing changes in colony color and clear zone formation and decolorization ability was determined by spectrophotometry. It was found that 2 out of the 15 isolated strains showed the highest copper resistance with the MIC of 7 mM. The two bacterial strains KIMS8 and KIMS10, were grown in solid media supplemented with 300 ppm of methylene blue or reactive black dye. Bacterial decolorization assays showed that KIMS8 was able to decolorize up to 90% methylene dye and up to 11.3% of reactive black dye whereas KIMS10 was able to decolorize 94.9% of methylene blue dye and 12.1% of reactive black dye. Molecular characterization by 16S rRNA gene sequencing of these two strains showed that KIMS8 and KIMS10 were identified as Enterobacter hormaechei and E. cloacae, respectively.

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Section: Dye-resistance and Decolorization Assaymentioning

confidence: 99%

Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes

Irawati

TIMOTHY²,

SOENTORO³

et al. 2023

Biodiversitas

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“…Absorbansi medium tanpa pewarna juga diukur sebagai kontrol. Persentase dekolorisasi dihitung dengan rumus sebagai berikut (Irawati et al, 2022) (Stackebrandt, 1994).…”

Section: Metode Penelitian Medium Pertumbuhanunclassified

Karakterisasi Resistensi dan Dekolorisasi Berbagai Pewarna Oleh Bakteri Indigen Indonesia Escherichia coli Strain CN5

Irawati

Adhiwijaya

Marvella³

2022

J. Biol Papua

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The development of the textile industry in Indonesia is increasing the amount of dye waste produced. Copper is often a component of dyes, both of which harm aquatic ecosystems because they cannot be degraded. These problems can be overcome by bioremediation using bacteria isolated from polluted waters, called indigenous bacteria. It is hoped that indigenous bacteria can degrade textile waste and reduce copper toxicity in waters. This study aims to characterize, test resistance, and test the decolorization of the Indonesian indigenous bacterial isolate CN5 to dye and copper. There are 12 kinds of dyes used, namely: methylene blue, malachite green, congo red, mordant orange, reactive black, direct yellow, basic fuchsin, reactive orange, disperse orange, remasol red, wantex yellow, and wantex red. Resistance and decolorization tests on solid medium were carried out by growing bacterial cultures into luria bertani agar medium, each of which added a different type of dye. The dye concentrations tested were 100 ppm and 500 ppm. The ability to decolorize is known from the presence of a clear zone around the bacterial colony. The percentage of decolorization was tested using a spectrophotometer at a wavelength of 300-900 nanometers. Bacterial identification was carried out by 16S-rRNA sequencing. The results showed that CN5 isolates had a base similarity of 100% with Escherichia coli, so hereinafter referred to as E. coli strain CN5 could grow at 200 ppm and 500 ppm methylene blue, malachite green, congo red, mordant orange, reactive black, direct yellow, reactive orange, disperse orange, red remasol, yellow wantex, and red wantex but did not grow on fuchsin basic dye. Colonies of E. coli strain CN5 were only able to decolorize methylene blue with a concentration of 200 ppm and 500 ppm seen from the clear zone formed around the colony. The decolorization of methylene blue that occurred was 92.47%. The addition of copper reduced the decolorization ability to 75.59%. Based on the results of this study, it can be concluded that the E. coli strain CN5 has the potential to be used as a bioremediation agent for textile waste containing copper and methylene blue.Key words: dye; E. coli; copper; decolorization; resistant

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“…Biakan bakteri diinkubasi dengan penggojogan 200 rpm pada suhu 37 o C. Kultur cair hasil inkubasi sebanyak 1 mL disentrifugasi pada 15.000 rpm selama 1 menit kemudian absorbansi supernatan diukur menggunakan spektrofotometer pada panjang gelombang 300-900 nanometer (nm). Persentase dekolorisasi dihitung dengan rumus sebagai berikut (Irawati et al, 2022):…”

Section: Uji Dekolorisasi Pada Berbagai Pewarnaunclassified

“…Hal ini menunjukkan bahwa tembaga menghambat pertumbuhan bakteri. Tembaga sebagai unsur mikro berperan penting sebagai kofaktor enzim, seperti sitokrom C oksidase, tetapi besifat toksik karena dapat mengikat protein melalui pembentukan jembatan disulfida sehingga mengakibatkan degradasi protein (Irawati et al, 2022).…”

Section: Pengaruh Tembaga Terhadap Pertumbuhan Bakteriunclassified

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Karakterisasi Bacillus cereus strain IrN yang memiliki sifat multiresistensi terhadap tembaga dan pewarna

Irawati¹,

Lucky

Tumakaka

et al. 2022

JBIOUNUD

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Peningkatan aktivitas industri tekstil semakin menambahkan jumlah limbah di lingkungan. Tembaga merupakan salah satu komponen pewarna sintetis sehingga limbah industri tekstil berpeluang mengandung pewarna dan tembaga. Bioremediasi menggunakan bakteri indigenus yang resisten terhadap pewarna dan tembaga diharapkan dapat meningkatkan efesiensi pengolahan limbah tekstil. Isolat bakteri IrN merupakan bakteri indigenus yang resisten terhadap tembaga dan diisolasi dari pengolahan limbah. Penelitian ini bertujuan untuk melakukan karakterisasi molekular serta menguji resistensi dan kemampuan dekolorisasi bakteri terhadap berbagai pewarna. Karakterisasi molekular dilakukan dengan analisis gen 16S rDNA. Medium yang digunakan adalah Luria Bertani dengan penambahan pewarna dan atau CuSO4 sebagai perlakuan. Pewarna yang digunakan ada 12 macam, yaitu: Methylene Blue, Malachite Green, Congo Red, Mordant Orange, Reactive Black, Direct Yellow, Basic Fuchsin, Reactive Orange, Disperse Orange, Remasol, Wantex Red, dan Wantex Yellow. Kemampuan dekolorisasi dan pertumbuhan bakteri diukur dengan menggunakan spektrofotometer. Hasil analisis 16S rDNA menunjukkan bahwa bakteri IrN teridentifikasi sebagai Bacillus cereus dengan kemiripan sebesar 99,91%. Isolat bakteri IrN memiliki multiresistensi terhadap 3 mM CuSO4 serta semua pewarna yang diuji pada konsentrasi 200 ppm, tetapi pada 500 ppm IrN hanya resisten terhadap methylene blue. Bakteri IrN dapat mendekolorisasi 200 ppm dan 500 ppm Methylene Blue masing-masing sebesar 42,78% dan 38,1%. Penambahan 3 mM CuSO4 pada medium 500 ppm Methylene BlueBlue tetap membuat bakteri mampu mendekolorisasi Methylene Blue, walaupun kemampuan dekolorisasinya menurun menjadi 34,17%. Isolat bakteri IrN juga dapat mendekolorisasi Malachite Green, Congo Red, Basic Fuchsine dengan kemampuan maksimal masing-masing sebesar 98,16%, 70,29%, dan 79,07%.

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The potential of copper-resistant bacteria Acinetobacter sp. strain CN5 in decolorizing dyes

Cited by 5 publications

References 34 publications

Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes

Enterobacter hormaechei KIMS8 and Enterobacter cloacae KIMS10 isolated from Kapuas River, Kalimantan, Indonesia as indigenous multi-resistant bacteria to copper and dyes

Karakterisasi Resistensi dan Dekolorisasi Berbagai Pewarna Oleh Bakteri Indigen Indonesia Escherichia coli Strain CN5

Karakterisasi Bacillus cereus strain IrN yang memiliki sifat multiresistensi terhadap tembaga dan pewarna

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