The potential of endophytic bacteria from Prosopis cineraria for the control of Pythium aphanidermatum-induced damping-off in cucumber under saline water irrigation

Al-Rashdi, Ahad; Al-Hinai, Fatma Sulaiman; Al-Harrasi, Majida Mohammed Ali; Al-Sabahi, Jamal Nasser; Al-Badi, Rahil Said; Al-Mahmooli, Issa Hashil; Al-Sadi, Abdullah M.; Velazhahan, R.

doi:10.1007/s42161-022-01237-5

Cited by 11 publications

(7 citation statements)

References 100 publications

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“…In contrast, the volatiles from B. firmus RWGB3 were not effective against A. flavus. The production of VOCs is recognized as an important mechanism by which antagonistic bacterial isolates suppress plant pathogenic fungi [ 41 , 42 , 56 , 83 , 84 , 85 , 86 ]. These VOCs are gaseous, lipophilic, low-molecular-weight (<300 Da) compounds [ 87 ] that disrupt fungal cell membranes, leading to the leakage of cell contents and, eventually, cell death [ 88 , 89 ].…”

Section: Discussionmentioning

confidence: 99%

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Al-Saadi,

Al-Sadi,

Al-Wahaibi

et al. 2024

JoF

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In this study, bacteria residing in the gut of the rice weevils (Sitophilus oryzae L.) (Coleoptera: Curculionidae) feeding on aflatoxin-contaminated corn kernels were isolated and evaluated for their ability to suppress Aspergillus flavus and to remove/degrade aflatoxin B1 (AFB1). Four morphologically distinct S. oryzae gut-associated bacterial isolates were isolated and identified as Bacillus subtilis (RWGB1), Bacillus oceanisediminis (RWGB2), Bacillus firmus (RWGB3), and Pseudomonas aeruginosa (RWGB4) based on 16S rRNA gene sequence analysis. These bacterial isolates inhibited A. flavus growth in the dual culture assay and induced morphological deformities in the fungal hyphae, as confirmed by scanning electron microscopy. All four bacterial isolates were capable of removing AFB1 from the nutrient broth medium. In addition, culture supernatants of these bacterial isolates degraded AFB1, and the degradation of toxin molecules was confirmed by liquid chromatography-mass spectrometry. The bacterial isolates, B. subtilis RWGB1, B. oceanisediminis RWGB2, and P. aeruginosa RWGB4, were capable of producing antifungal volatile organic compounds that inhibited A. flavus growth. These results suggest that the bacterial isolates from S. oryzae gut have the potential to bind and/or degrade AFB1. Further research on their application in the food and feed industries could enhance the safety of food and feed production.

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Section: Discussionmentioning

confidence: 99%

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Al-Saadi,

Al-Sadi,

Al-Wahaibi

et al. 2024

JoF

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“…Further, by measuring the lesion diameter, the severity of the diseases has been assessed to determine the effectiveness of the biocontrol agent. Although, during the inoculation, either the pathogens or microbial antagonistic are inoculated at fixed time intervals [95,99].…”

Section: In Vivo Methodsmentioning

confidence: 99%

Biocontrol Screening of Endophytes: Applications and Limitations

Kashyap

Singh

Yadav

et al. 2023

Plants

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The considerable loss of crop productivity each year due to plant disease or pathogen invasion during pre- or post-harvest storage conditions is one of the most severe challenges to achieving the goals of food security for the rising global population. Although chemical pesticides severally affect the food quality and health of consumers, a large population relies on them for plant disease management. But currently, endophytes have been considered one of the most suitable biocontrol agents due to better colonization and acclimatization potential. However, a very limited number of endophytes have been used commercially as biocontrol agents. Isolation of endophytes and their screening to represent potential characteristics as biocontrol agents are considered challenging by different procedures. Through a web search using the keywords “endophytes as biocontrol agents” or “biocontrol mechanism of endophytes,” we have succinctly summarised the isolation strategies and different in vitro and in vivo biocontrol screening methods of endophytic biocontrol agents in the present review. In this paper, biocontrol mechanisms of endophytes and their potential application in plant disease management have also been discussed. Furthermore, the registration and regulatory mechanism of the endophytic biocontrol agents are also covered.

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“…Various bacterial endophytes have been reported to control Pythium blight in cucumber [45][46][47][48][49]. They have shown different modes of action on Pythium species.…”

Section: Plos Onementioning

confidence: 99%

“…against several plant pathogens including Pythium aphanidermatum in tomato by secreting different metabolites [46]. Four bacterial endophytes isolated from halophytic plant Prosopis cineraria showed potential to control P. aphanidermatum in vitro [47] and one of them inhibited the pathogen by releasing volatile organic compounds. There was a dearth of peerreviewed articles of bacterial endophytes controlling Pythium decline in hydroponic lettuce.…”

Section: Plos Onementioning

confidence: 99%

Biocontrol potential of endophytic Pseudomonas strain IALR1619 against two Pythium species in cucumber and hydroponic lettuce

Amaradasa,

Mei,

et al. 2024

PLoS ONE

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The use of fungicides to manage disease has led to multiple environmental externalities, including resistance development, pollution, and non-target mortality. Growers have limited options as legacy chemistry is withdrawn from the market. Moreover, fungicides are generally labeled for traditional soil-based production, and not for liquid culture systems. Biocontrol agents for disease management are a more sustainable and environmentally friendly alternative to conventional agroprotectants. Pythium ultimum is a soil borne oomycete plant pathogen with a broad taxonomic host range exceeding 300 plants. Cucumber seedlings exposed to P. ultimum 1 day after a protective inoculation with bacterial endophyte accession IALR1619 (Pseudomonas sp.) recorded 59% survival; with the control assessed at 18%. When the pathogen was added 5 days post endophyte inoculation, 74% of the seedlings treated survived, compared to 36% of the control, indicating a longer-term effect of IALR1619. Under hydroponic conditions, IALR1619 treated leaf type lettuce cv. ‘Cristabel’ and Romaine cv. ‘Red Rosie’ showed 29% and 42% higher shoot fresh weight compared to their controls, respectively. Similar results with less growth decline were observed for a repeat experiment with IALR1619. Additionally, an experiment on hydroponic lettuce in pots with perlite was carried out with a mixture of P. ultimum and P. dissotocum after IALR1619 inoculation. The endophyte treated ‘Cristabel’ showed fresh weight gain, but the second cultivar ‘Pensacola’ yielded no increase. In summary, the endophyte IALR1619 provided short term as well as medium-term protection against Pythium blight in cucumber seedlings and may be used as an alternative to conventional fungicides in a greenhouse setting. This study also demonstrated the potential of ALR1619 as a biocontrol agent against Pythium blight in hydroponic lettuce.

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The potential of endophytic bacteria from Prosopis cineraria for the control of Pythium aphanidermatum-induced damping-off in cucumber under saline water irrigation

Cited by 11 publications

References 100 publications

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Biocontrol Screening of Endophytes: Applications and Limitations

Biocontrol potential of endophytic Pseudomonas strain IALR1619 against two Pythium species in cucumber and hydroponic lettuce

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