THE POTENTIAL OF ENDOPHYTIC Trichoderma FROM OIL PALM (Elaeis guineensis Jacq.) ROOTS OF NORTH SUMATRA, INDONESIA AGAINST Ganoderma boninense

Abstract

Utilisation of endophyticTrichoderma increases tremendously as an alternative control against G. boninense, causal pathogen of basal stem rot (BSR) disease of oil palm. However, investigation of endophytic Trichoderma from Indonesia is still very scarce. The aims of this study were to isolate, identify and investigate the potential of endophytic Trichoderma from oil palm roots. Three potential endophytic Trichoderma species were isolated and further identified using macroscopic, microscopic, and molecular methods. Antagonistic activities of endophytic Trichoderma were tested using dual culture agar and poison food agar assay. A molecular approach using DNA sequencing of 5.8S-ITS region successfully identified the endophytic Trichoderma isolate ET501 as Trichoderma reesei strain RHa, while isolates of endophytic ET523 and ET537 were identified as Trichoderma asperellum isolate F1 and Trichoderma asperellum strain Q1, respectively. Trichoderma reesei ET501 was the most aggressive isolate against G. boninense with PIRG of 95.1% compared to T. asperellum ET523 and T. asperellum ET537 with PIRG of 87.1% and 88.9%, respectively. Meanwhile, T. reesei ET501 showed the strongest antibiosis activity with 100% inhibition in 80% concentration, compared to T. asperellum ET523 and T. asperellum ET537 which gave 12.3% and 90.5% of inhibition, respectively.

Actividad enzimática ligninolítica de dos poblaciones de Ganoderma spp. en interacción con Trichoderma spp.

“…Por otra parte, el género Ganoderma spp. tiene características ecológicas versátiles al poseer un hábito saprobio y fitopatógeno; algunas especies son de interés para su control biológico con hongos antagonistas como especies del género Trichoderma (Naher et al 2012b, Sundram 2013a, Sundram 2013b. Sin embargo, en la bibliografía científica hay pocas investigaciones sobre el efecto de esas interacciones en la actividad enzimática ligninolítica de los géneros en cuestión, por lo tanto, el presente estudio representa una contribución al tema.…”

Comparative incidence of Ganoderma basal stem rot and upper stem rot disease in oil palm in relation to soil type and geographical distribution

“…The fungal isolation kit's modified mini technique was used to isolate total DNA from all 83 pure isolate cultures. The DNA extraction was done using the Invitrogen DNA isolation kit with minor modification, as done by Haryadi [20]. In addition, a multiplex polymerase chain reaction (PCR) was performed following the PCR protocol as described by Boyle et al [21], with ITS1 and ITS4 as primers.…”

Detection, characterization, and management of plant pathogens

“…Their mechanisms of action toward the target pathogen can be varying either directly or indirectly, such as competition, predation, antibiosis, induced host resistance, or by the activity of lytic enzymes. Over the past decades, numerous research has been published on the potential application of beneficial microorganisms as biological control agents (BCAs) against plant pathogenic bacteria, and strains from the genera Pseudomonas, Burkholderia, Streptomyces, Bacillus, and Trichoderma are very wellknown for their antimicrobial capability and synthesis of a wide range of bioactive compounds (Alexander and Phin, 2014;Haryadi et al, 2019;Lim et al, 2019;Alexander et al, 2021).…”