The potential of FT-IR spectroscopy for improving healthcare in sepsis – An animal model study

Pinto, G.; Leal, Leonardo Barbosa; Magalhães, N.C.; Pereira, Marcos; Vassallo, Paula Frizera; Pereira, Thiago Marinho; Baraúna, Valério Garrone; Byrne, Hugh J.; Carvalho, Luís Felipe C. S.

doi:10.1016/j.pdpdt.2021.102312

Cited by 4 publications

(1 citation statement)

References 57 publications

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“…This technique has been successfully tested as a tool for screening, diagnostics, and progression of diseases by examining several bio uid samples, including cells and tissue [9,10]. Our group has previously shown the applicability of ATR-FTIR in bio uids, in both animal and human models, as screening for COVID-19 [11][12][13], iron overload [14], sepsis severity [15], and cancer [16]. Another metabolic genetic disease has also been studied (galactosemia), and ATR-FTIR successfully discriminated among diabetic, healthy and galactosemic subjects.…”

Section: Introductionmentioning

confidence: 99%

Infrared spectroscopy as a new approach for Fabry disease screening

Barretto,

Nascimento,

Brun

et al. 2023

Preprint

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Background Fabry disease (FD) is a rare X-linked lysosomal storage disorder marked by alpha-galactosidase-A (α-Gal A) deficiency, caused by pathogenic mutations in the GLA gene resulting in the accumulation of glycosphingolipids inside lysosomes. The current screening test consists of measuring α-Gal A activity. However, it is limited only to men. Infrared spectroscopy is a technique that provides information about biofluids' molecular composition and has been successfully applied in numerous diseases. Herein, we investigate the vibration profile of plasma chemical bonds in patients with FD through attenuated total reflectance Fourier transform infrared (ATR-FTIR) vibrational spectroscopy. Results The Fabry disease group (n = 47) and the healthy control group (n = 52) were analyzed with similar ages (39.2 ± 16.9 and 36.7 ± 10.9 years, respectively), and women were predominant in both groups (59,6% vs. 65,4%). All patients had the classic phenotype (100%), and no late-onset phenotype was detected. PLS-DA classification model independent of gender allowed differentiation of the samples between Fabry and the control group, reaching 100% sensitivity, specificity, and accuracy. Conclusion ATR-FTIR spectroscopy associated with pattern recognition can distinguish between FD patients and healthy control participants as a fast-screening test.

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Section: Introductionmentioning

confidence: 99%

Infrared spectroscopy as a new approach for Fabry disease screening

Barretto,

Nascimento,

Brun

et al. 2023

Preprint

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Infrared spectroscopy combined with chemometrics in transflectance mode: An alternative approach in the photodiagnosis of COVID-19 using saliva

Juchem,

Corbellini,

Horst

et al. 2024

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Infrared spectroscopy as a new approach for early fabry disease screening: a pilot study

Barretto,

Nascimento,

Brun

et al. 2024

Orphanet J Rare Dis

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Background Fabry disease (FD) is a rare X-linked lysosomal storage disorder marked by alpha-galactosidase-A (α-Gal A) deficiency, caused by pathogenic mutations in the GLA gene, resulting in the accumulation of glycosphingolipids within lysosomes. The current screening test relies on measuring α-Gal A activity. However, this approach is limited to males. Infrared (IR) spectroscopy is a technique that can generate fingerprint spectra of a biofluid’s molecular composition and has been successfully applied to screen numerous diseases. Herein, we investigate the discriminating vibration profile of plasma chemical bonds in patients with FD using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy. Results The Fabry disease group (n = 47) and the healthy control group (n = 52) recruited were age-matched (39.2 ± 16.9 and 36.7 ± 10.9 years, respectively), and females were predominant in both groups (59.6% and 65.4%, respectively). All patients had the classic phenotype (100%), and no late-onset phenotype was detected. A generated partial least squares discriminant analysis (PLS-DA) classification model, independent of gender, allowed differentiation of samples from FD vs. control groups, reaching 100% sensitivity, specificity and accuracy. Conclusion ATR-FTIR spectroscopy harnessed to pattern recognition algorithms can distinguish between FD patients and healthy control participants, offering the potential of a fast and inexpensive screening test.

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The potential of FT-IR spectroscopy for improving healthcare in sepsis – An animal model study

Cited by 4 publications

References 57 publications

Infrared spectroscopy as a new approach for Fabry disease screening

Infrared spectroscopy as a new approach for Fabry disease screening

Infrared spectroscopy combined with chemometrics in transflectance mode: An alternative approach in the photodiagnosis of COVID-19 using saliva

Infrared spectroscopy as a new approach for early fabry disease screening: a pilot study

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