The Potential of Hydrocarbon Chemotaxis to Increase Bioavailability and Biodegradation Efficiency

Lacal, Jesús

doi:10.1007/978-3-319-20796-4_3-1

Cited by 2 publications

(2 citation statements)

References 69 publications

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“…This allows us to explore disparate specialized niches and communal behavior of bacteria and the pore-scale hydrodynamics that influence their habitat. The patchy distribution of microcolonial bacteria that appeared to be most metabolically active is suggestive of heterogeneous resource allocation. , This may reflect the heterogeneous distribution of diesel and/or nutrients within the soil, which is common in PHC-contaminated soils . Biofilm bacteria typically form through production of extracellular polymeric substances and can dramatically alter the fluid dynamics of their environment .…”

Section: Discussionmentioning

confidence: 99%

“…81,82 This may reflect the heterogeneous distribution of diesel and/or nutrients within the soil, which is common in PHCcontaminated soils. 83 Biofilm bacteria typically form through production of extracellular polymeric substances and can dramatically alter the fluid dynamics of their environment. 84 Live and/or dead bacteria may clog preferential flow paths, effectively remodeling the porous network within the soil, with implications for chemical transport in natural porous media.…”

Section: ■ Discussionmentioning

confidence: 99%

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Soil Buffering Capacity Can Be Used To Optimize Biostimulation of Psychrotrophic Hydrocarbon Remediation

Mamet

Jimmo

Conway

et al. 2021

Environ. Sci. Technol.

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Effective bioremediation of hydrocarbons requires innovative approaches to minimize phosphate precipitation in soils of different buffering capacities. Understanding the mechanisms underlying sustained stimulation of bacterial activity remains a key challenge for optimizing bioremediationparticularly in northern regions. Positron emission tomography (PET) can trace microbial activity within the naturally occurring soil structure of intact soils. Here, we use PET to test two hypotheses: (1) optimizing phosphate bioavailability in soil will outperform a generic biostimulatory solution in promoting hydrocarbon remediation and (2) oligotrophic biostimulation will be more effective than eutrophic approaches. In so doing, we highlight the key bacterial taxa that underlie aerobic and anaerobic hydrocarbon degradation in subarctic soils. In particular, we showed that (i) optimized phosphate bioavailability outperformed generic biostimulatory solutions in promoting hydrocarbon degradation, (ii) oligotrophic biostimulation is more effective than eutrophic approaches, and (iii) optimized biostimulatory solutions stimulated specific soil regions and bacterial consortia. The knowledge gleaned from this study will be crucial in developing field-scale biodegradation treatments for sustained stimulation of bacterial activity in northern regions.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Soil Buffering Capacity Can Be Used To Optimize Biostimulation of Psychrotrophic Hydrocarbon Remediation

Mamet

Jimmo

Conway

et al. 2021

Environ. Sci. Technol.

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Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota

et al. 2018

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While most studies using RNA-stable isotope probing (SIP) to date have focused on ribosomal RNA, the detection of 13C-labeled mRNA has rarely been demonstrated. This approach could alleviate some of the major caveats of current non-target environmental “omics.” Here, we demonstrate the feasibility of total RNA-SIP in an experiment where hydrocarbon-degrading microbes from a BTEX-contaminated aquifer were studied in microcosms with 13C-labeled toluene under microoxic conditions. From the total sequencing reads (∼30 mio. reads per density-resolved RNA fraction), an average of 1.2% of reads per sample were identified as non-rRNA, including mRNA. Members of the Rhodocyclaceae (including those related to Quatrionicoccus spp.) were most abundant and enriched in 13C-rRNA, while well-known aerobic degraders such as Pseudomonas spp. remained unlabeled. Transcripts related to cell motility, secondary metabolite formation and xenobiotics degradation were highly labeled with 13C. mRNA of phenol hydroxylase genes were highly labeled and abundant, while other transcripts of toluene-activation were not detected. Clear labeling of catechol 2,3-dioxygenase transcripts supported previous findings that some of these extradiol dioxygenases were adapted to low oxygen concentrations. We introduce a novel combination of total RNA-SIP with calculation of transcript-specific enrichment factors (EFs) in 13C-RNA, enabling a targeted approach to process-relevant gene expression in complex microbiomes.

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The Potential of Hydrocarbon Chemotaxis to Increase Bioavailability and Biodegradation Efficiency

Cited by 2 publications

References 69 publications

Soil Buffering Capacity Can Be Used To Optimize Biostimulation of Psychrotrophic Hydrocarbon Remediation

Soil Buffering Capacity Can Be Used To Optimize Biostimulation of Psychrotrophic Hydrocarbon Remediation

Transcriptome-Stable Isotope Probing Provides Targeted Functional and Taxonomic Insights Into Microaerobic Pollutant-Degrading Aquifer Microbiota

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