The potential of Megasphaera elsdenii isolates to control ruminal acidosis

Henning, Pieter; Horn, C.H.; Steyn, D. G.; Meissner, H.H.; Hagg, F.M.

doi:10.1016/j.anifeedsci.2009.12.011

Cited by 51 publications

(44 citation statements)

References 13 publications

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“…Horn et al (2009a;b) selected from high producing dairy cows a number of Megasphaera elsdenii strains which have the potential to utilise lactic acid. From these strains one was selected (Henning et al, 2010a) and patented as M.e. strain NCIMB 41125.…”

Section: Introductionmentioning

confidence: 99%

Effect of Megasphaera elsdenii NCIMB 41125 drenching on health and performance of steers fed high and low roughage diets in the feedlot

Leeuw¹,

Siebrits²,

Henning³

et al. 2010

SA J. An. Sci.

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________________________________________________________________________________ AbstractLactate utilising bacteria (LUB) assist in reducing the risk of ruminal lactate acidosis when high concentrate diets are fed to feedlot cattle. Ruminal lactate acidosis can lead to lower animal performance and morbidity. Preliminary studies suggested that the strain, Megasphaera elsdenii (M.e.) NCIMB 41125, is a particularly potent LUB. The potential of M.e.NCIMB 41125 to improve the health and performance of feedlot cattle was investigated. Four hundred and forty eight Bonsmara steers (ca. 240 kg) were used in a 100-day feeding trial. Half the steers received at processing 200 mL M.e.NCIMB 41125 per os (LY) and the other half no LUB (LN). The diets in each of these treatments were divided into a low roughage (2%) (RL) and high roughage (8%) (RH) diet. The effects of LY vs. LN, RL vs. RH as main effects and their respective interactions (LYRL, LYRH, LNRL & LNRH) were compared in terms of morbidity, feedlot performance and carcass characteristics. The steers were weighed at two week intervals, feed was offered daily and the orts were removed weekly from each pen. The faecal consistency score and incidence of morbidity were recorded. At slaughter, carcass data were collected and the health status of the liver and rumen epithelium was assessed. Steers dosed with M.e.NCIMB 41125 had a 5.6% better average daily gain (ADG) during weeks 3 -5 (2.09 kg/day vs. 1.98 kg/day for LY and LN, respectively). Feed conversion ratio (FCR, Weeks 1 -13) was better for the steers fed the RL than the RH treatment (4.72 kg/kg vs. 4.99 kg/kg for RL and RH, respectively). Steers on the LNRH treatment during weeks 3 -5 used more feed per kg gain than steers on the other treatments (5.39 kg/kg for LNRH vs. 4.74 kg/kg and 4.72 kg/kg for LYRL and LNRL, respectively). More steers (21) on the LNRL treatment were treated for morbidity than on the other treatments (8, 7 and 5 for LYRL, LYRH and LNRH, respectively). In general, animal performance was not improved by dosing with M.e.NCIMB 41125, but since ADG was improved in the immediate postadaptation phase (weeks 3 -5) and morbidity levels were lower on the low roughage diet, dosing of steers on low roughage, lactate acidosis-prone, diets with M.e.NCIMB 41125 should prove useful. ________________________________________________________________________________

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Section: Introductionmentioning

confidence: 99%

Effect of Megasphaera elsdenii NCIMB 41125 drenching on health and performance of steers fed high and low roughage diets in the feedlot

Leeuw¹,

Siebrits²,

Henning³

et al. 2010

SA J. An. Sci.

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“…Finally, we observed a significant increment of concentration (P=0.003) during the in vitro fermentation, which was not found for this strain by Weimer et al (2011). The fourth bacteria considered, M. elsdenii, utilises lactate which helps it to adapt favourably to concentrate diets and to mitigate the acidic conditions of the rumen (Henning et al, 2010a;2010b). In the first fermentation run this bacteria was not detected in rumen inoculum from cows, while in second run the concentrations were negible or very low (0.14±0.07×10 4 copies /mL).…”

Section: Resultsmentioning

confidence: 69%

The Use of Quantitative Real Time Polymerase Chain Reaction to Quantify Some Rumen Bacterial Strains in anIn VitroRumen System

Onime

Zanfi

Agostinis

et al. 2013

Italian Journal of Animal Science

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The aim of this work was to quantify four rumen bacterial strains (Butyrivibrio fibrisolvens, Ruminococcus albus, Streptococcus bovis, Megasphaera elsdenii) in an in vitro batch rumen fermentative system by quantitative real time polymerase chain reaction (qPCR). The experiment was a 2×2 factorial arrangement with two types of liquid rumen, collected from dairy cows (DC) and fattening bulls (FB) and two types of fermentation substrate (forage:concentrate ratios, 75:25 and 25:75) and was replicated in two fermentation runs. Fermentation fluids from FB compared to those from DC had lower pH, higher total VFA concentrations (averages of 0 and 24 h samplings, 6.70 vs 7.04 and 72.6 vs 42.7 mmol/l P<0.001) and contained less acetic (P=0.014) and more propionic (P<0.01) and butyric (P=0.029) acids. The two types of substrates incubated produced very small differences in the end fermentation products.B. fibrosolvens concentrations were higher (P<0.001) in the DC fermentation fluids compared to that from bulls (averages of 0 and 24 h sampling times, 3.47 vs 1.38 x10 9 copies /mL), while M. elsdenii was detected only in FB fermentation fluids. R. albus and S. bovis concentrations were not different between the two types of rumen liquid. With the only exception for B. fibrosolvens, bacteria strains considered in this study increased their concentrations in the fermentation fluid during the 24 h of in vitro incubation.

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“…On d 7 (prior to treatment administration), BHBA concentrations tended (p = 0.09) to be greater for Meg0 calves compared to Meg14 calves; however, BHBA concentration increased The potential of M. elsdenii to increase the proportion of rumen propionate and butyrate was previously reported (Marounek et al, 1989;Cruz et al, 2001;Henning et al, 2010;Muya et al, 2015). The particular role of these two VFA on stimulating rumen epithelial cells and papillae development and thereby, increasing the capacity of solid feed intake, has been previously discussed (Tamate et al, 1962;Coverdale et al, 2004;Lane and Jesse, 1997).…”

Section: Blood Beta-hydroxybutyratementioning

confidence: 75%

“…Butyrate and propionate are the main volatile fatty acids (VFA) responsible for rumen epithelial and papillae development in the pre-weaned ruminant (Coverdale et al, 2004). Megasphaera elsdenii is thought to play a major role in production of branchedchain of VFA in the rumen (Wallace, 1986) and alter rumen fermentation in favour of propionate and butyrate as end products (Henning et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Performance of Holstein calves having free access to milk and dosed with Megasphaera elsdenii

Muya

Erasmus

Miller

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Megasphaera elsdenii converts lactate and glucose into butyrate, the main volatile fatty acid responsible of papillae development and may benefit calf performance. Twenty-six Holstein calves (BW = 34.5 ± 1.65 kg) were randomly assigned at birth to a control group (Meg0) and a group that received an oral dose of M. elsdenii NCIMB 41125 at 14 d of age (Meg14).Calves received colostrum for the first 3 d followed by free choice access to whole milk until weaning at 56 d. From d 4 onward, starter and water were offered ad libitum. Intakes were measured daily and body weights (BW) weekly. Blood samples were collected on day 7, 21, 28, 42, and 56 for β-hydroxybutyrate (BHBA) analysis. Performance was measured for an additional 14 d post-weaning. Pre-weaning milk intake was lower (p = 0.010) and starter DMI (dry matter intake) greater (p = 0.001) for Meg14 than Meg0 calves. Total DMI, metabolisable energy (ME) intake and average daily gain (ADG) were similar (p > 0.05) for both groups, but Meg14 calves had greater weaning BW (p = 0.012) and feed efficiency (p < 0.029). The average BHBA between d 21 and 56 was greater for Meg14 (p = 0.03) compared to Meg0 calves. After weaning, Meg14 calves had greater DMI (p = 0.027), ME intake (p = 0.023) and ADG (p = 0.002) and tended to have better feed efficiencies (p = 0.07) than Meg0 calves. Administering M. elsdenii NCIMB 41125 improved starter intake and feed efficiency, which was associated with high blood BHBA.

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The potential of Megasphaera elsdenii isolates to control ruminal acidosis

Cited by 51 publications

References 13 publications

Effect of Megasphaera elsdenii NCIMB 41125 drenching on health and performance of steers fed high and low roughage diets in the feedlot

Effect of Megasphaera elsdenii NCIMB 41125 drenching on health and performance of steers fed high and low roughage diets in the feedlot

The Use of Quantitative Real Time Polymerase Chain Reaction to Quantify Some Rumen Bacterial Strains in anIn VitroRumen System

Performance of Holstein calves having free access to milk and dosed with Megasphaera elsdenii

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