The Potential of Optimized Liposomes in Enhancement of Cytotoxicity and Apoptosis of Encapsulated Egyptian Propolis on Hep-2 Cell Line

Abstract

Purpose: Development of pharmaceutical dosage forms of natural products has gained great interest recently. Propolis is a natural product with various active compounds and multiple pharmacological activities. Its resinous nature and low bioavailability were obstacles in the optimum use of this magnificent natural product. Aim: This study evaluates the effect of using liposomes as a drug delivery system on the enhancement of the cytotoxic effect of propolis on squamous cell carcinoma cell lines (Hep-2) of head and neck. Methods: An optimized liposomal formulation of propolis was prepared using the conventional thin film hydration method 1, 2. The prepared (Hep-2) cell line was treated with different concentrations of propolis and optimized propolis liposomes for 24 h. The effect of both propolis and propolis liposomes on cell line was investigated using MTT assay, cytological examination, and nuclear morphometric analysis. The effect of the drugs on the cell apoptosis was evaluated using Annexin V. Results: The findings revealed that both propolis and propolis liposomes have a cytotoxic effect on Hep-2 cell line through induction of apoptosis. The effect was dose dependent. However, a statistically significant enhancement in propolis-mediated apoptosis on Hep-2 cells was elucidated due to encapsulation within the prepared liposomes. Conclusion: Liposome is a powerful tool for enhancing the cytotoxicity of propolis against Hep-2 cell line.

Monascus Red Pigment Liposomes: Microstructural Characteristics, Stability, and Anticancer Activity

“…MRPL appeared to be a predominantly spherical UV with even distribution, and the particles' size varied from 20 to 200 nm with small vesicles occupying the majority, which is consistent with previous studies that reported that nanoliposomes possess a morphology of spherical vesicles along with a particle size varying from tens to hundreds [11,[24][25]. Some reports emphasized the bigger particle size of liposomes having a positive connection with the increasing cholesterol content [16,26]. The particle size is also influenced by prepared methods, such as extrusion and sonication technique [16].…”

Comparative Study of Antiviral, Cytotoxic, Antioxidant Activities, Total Phenolic Profile and Chemical Content of Propolis Samples in Different Colors from Turkiye

“…It has been proven in the literature that propolis has cytotoxic activity against Hep-2 (squamous cell carcinoma cell line), Caco-2 (human colon adenocarcinoma), HL60 (human promyelocytic leukemia), MG63 (human osteosarcoma), A549 (human lung adenocarcinoma cell line), MDA-MB-231 (breast cancer cell line), PANC-1 (human pancreatic cancer cell line), HeLa (epitheloid cervix carcinoma) and MCF7 (breast cancer cell line). However, its activity against cell lines HeLa, MCF7 and A549 stands out [ 43 , 44 , 45 ]. For this purpose, the cytotoxicity results of different color propolis extracts were discussed through a panel of cancerous and nontumor cells in this study.…”

Alginate as a Promising Biopolymer in Drug Delivery and Wound Healing: A Review of the State-of-the-Art

“…Numerous of them grasp magnificent therapeutic properties; nevertheless, they manifest poor bioavailability and poor pharmacokinetics, causing detrimental systemic impact after the administration [ 117 ]. Therefore, DDS has evolved into an essential device to enhance remedial effectiveness by employing delivery carriers (as illustrated in Figure 4 ) such as microspheres, hydrogels, beads, liposomes, nanoparticles, nanofibers, etc., to deliver drugs [ 118 , 119 , 120 ].…”