The potential of plastid trnL (UAA) intron polymorphisms for the identification of the botanical origin of plant oils

Spaniolas, Stelios; Bazakos, Christos; Thodhoraq, Spano; Zoghby, Claire; Kalaitzis, Panagiotis

doi:10.1016/j.foodchem.2010.02.039

Cited by 45 publications

(35 citation statements)

References 17 publications

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“…Moreover, barcode size did not vary among the five olive cultivars, providing proof of the species specificity of trnL (UAA) intron length polymorphisms. Our results were in agreement with Spaniolas, Bazakos, Awad, et al (2008), Spaniolas, Bazakos, Spano, et al (2010), who demonstrated length polymorphisms of the barcode amplicon among olive, soybean, sunflower, sesame, cotton, corn and hazelnut. Barcode fragment length polymorphisms among olive and the remaining four oil seed species (palm, rapeseed, peanut and safflower) are shown for the first time in the present research.…”

Section: Pcr-ce Analysis Of Reference Tissue and Oil Samplessupporting

confidence: 93%

“…Interspecific length polymorphisms in the plastid trnL (UAA) intron were previously proposed for botanical origin authentication in plant oils (Spaniolas, Bazakos, Awad, et al, 2008;Spaniolas, Bazakos, Spano, et al, 2010). However, the applicability of a barcode amplicon size separation assay to discriminate species-specific fragments in oil blends was not investigated.…”

Section: Pcr-ce Analysis and Fatty Acid Profiling Of Plant Oil Admixtmentioning

confidence: 99%

“…The plastid genome is predominantly used as an analytical target in such work (Ganopoulos, Bazakos, Madesis, Kalaitzis, & Tsaftaris, 2013;Kumar, Kahlon, & Chaudhary, 2011;Li et al, 2012;Spaniolas, Bazakos, Awad, & Kalaitzis, 2008;Spaniolas, Bazakos, Spano, Zoghby, & Kalaitzis, 2010;Vietina et al, 2013;Wu et al, 2011;Zhang et al, 2012), as its high copy number in plant cells enables the recovery of a sufficient quality and quantity of DNA. Moreover, target amplification from a diverse range of taxa is achieved due to the conserved gene order and content of the plastid genome, which allow the design of universal primers (Uncu, Uncu, Celik, Doganlar, & Frary, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the high rate of insertions/deletions (Gielly & Taberlet, 1994) in the noncoding region can be translated into discriminative amplicon size separation assays that allow the identification of species composition in food products. Spaniolas, Bazakos, Awad, et al (2008), Spaniolas, Bazakos, Spano, et al (2010) were first to describe the potential of the trnL (UAA) intron to authenticate the botanical origin of plant oils. The authors demonstrated the presence of discriminative length polymorphisms in the barcode amplicon among oil crop species.…”

Section: Introductionmentioning

confidence: 99%

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Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil

Uncu

Frary

et al. 2017

Food Chemistry

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a b s t r a c tThe aim of this study was to compare the performance of a DNA-barcode assay with fatty acid profile analysis to authenticate the botanical origin of olive oil. To achieve this aim, we performed a PCRcapillary electrophoresis (PCR-CE) approach on olive oil: seed oil blends using the plastid trnL (UAA) intron barcode. In parallel to genomic analysis, we subjected the samples to gas chromatography analysis of fatty acid composition. While the PCR-CE assay proved equally efficient as gas chromatography analysis in detecting adulteration with soybean, palm, rapeseed, sunflower, sesame, cottonseed and peanut oils, it was superior to the widely utilized analytical chemistry approach in revealing the adulterant species and detecting small quantities of corn and safflower oils in olive oil. Moreover, the DNA-based test correctly identified all tested olive oil: hazelnut oil blends whereas it was not feasible to detect hazelnut oil adulteration through fatty acid profile analysis. Thus, the present research has shown the feasibility of a PCR-CE barcode assay to detect adulteration in olive oil.

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Section: Pcr-ce Analysis Of Reference Tissue and Oil Samplessupporting

confidence: 93%

Section: Pcr-ce Analysis and Fatty Acid Profiling Of Plant Oil Admixtmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil

Uncu

Frary

et al. 2017

Food Chemistry

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“…Recently, non-conventional methods based on DN" analysis have gained atention due to their high speciicity, sensitivity and accuracy to detect the varietal origin of olive oil as well as the botanical origin of plant oils [12][13][14][15]. Moreover, DN"-based methods overcome deiciencies of conventional methods such as denaturation of proteins due to the heating and processing of food commodities [16].…”

mentioning

confidence: 99%

DNA-Based Approaches for Traceability and Authentication of Olive Oil

Bazakos¹,

Spaniolas²,

Kalaitzis³

2016

Products From Olive Tree

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Abstract"uthentication and traceability of extra virgin olive oil is a challenging research task due to the complexity of fraudulent practices. Various chemical and biochemical techniques have been developed for determining the authenticity of olive oil and in recent years non-conventional methods based on DN" analysis have gained atention, due to high speciicity, sensitivity and reliability. DN" analyses have very high discriminating power because ultimately the unique identity of a variety or species is to a great extent genetically dependent. Polymorphisms are genetic variations which refer to the variation in populations or species. Molecular markers provide information on genetic variations and are valuable tools to determine olive oil authenticity. Recently several DN"-based methods have been developed to authenticate olive oil, since analysis of the residual oil DN" with the use of molecular markers can lead to the identiication of the variety or the plant species from which it was extracted. The aim of this chapter is to provide an overview of the current trends and critical issues on DN"-targeted approaches used for traceability and authenticity of olive oil. This is considered a rapidly expanding ield with signiicant challenges and prospects which shall be discussed thoroughly.

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