The potential of Zishen Yutai pills to facilitate endometrial recovery and restore fertility after induced abortion in rats

Li, Mianmian; Ning, Na; Liu, Yu; Li, Xiaohui; Mei, Qiaojuan; Huang, Qiuling; Xiang, Wenpei; Zhang, Ling; Xu, Xiaoyan

doi:10.1080/13880209.2021.1993272

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…The clinical dose of ZYP (Baiyunshan Zhongyi Pharmaceutical Co. Ltd, China) is 15 g/day [ 14 ]. The equivalent dose for rats is about 1.575 g/kg of body weight per day, as confirmed by a previous report [ 15 ]. ZYP or saline solution was administered intragastrically once per day, for one week.…”

Section: Methodssupporting

confidence: 85%

Regulatory effects of Zishen Yutai Pill on endometrial epithelial response in vitro in immunology microenvironment

Chen,

Xie,

et al. 2023

Heliyon

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Section: Methodssupporting

confidence: 85%

Regulatory effects of Zishen Yutai Pill on endometrial epithelial response in vitro in immunology microenvironment

Chen,

Xie,

et al. 2023

Heliyon

Self Cite

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“…26 Previous studies showed that ZYP increased the expression of VEGF in the endometrium of postabortion rat model, promoted endometrial blood supply, and accelerated the recovery of endometrium. 27 In the present experiment, both in vivo and in vitro experiments, ZYP increased the expression of VEGF, VEGF-R1 and VEGF-R2, improved the viability of PTCs, and promoted the angiogenesis of maternal-fetal interface. miRNAs, a class of endogenous non-coding RNAs, which are involved in a variety of diseases.…”

Section: Discussionsupporting

confidence: 46%

Zishen Yutai Pills Promote Angiogenesis at the Maternal-Fetal Interface in Recurrent Spontaneous Abortion Mice by Regulating miR-187/VEGF Axis

Wang,

Hu,

et al. 2024

DDDT

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Zishen Yutai pills (ZYP), a traditional Chinese patent medicine, was listed in China in 1981. It is composed of 15 traditional Chinese medicines and has the effects of regulating menstruation, helping pregnancy, and preventing abortion. In clinical practice, it is effective in preventing habitual and threatened miscarriages, and continuing to explore its mechanism of action is very meaningful research. Aim of the Study:To explore the possible mechanism of ZYP promoting angiogenesis at the maternal-fetal interface in recurrent spontaneous abortion (RSA). Materials and Methods:In vitro experiments, placental trophoblast cells (PTCs) were isolated from the placental tissue of RSA mice and divided into six groups: Control group, Model group, ZYP group, miR-187 inhibitor NC group, miR-18 7 inhibitor group, and miR-187 inhibitor+ZYP group. Cell viability and cell cycle were measured using CCK8 and flow cytometry, respectively. The expression levels of miR-187, VEGF, VEGF-R1, and VEGF-R2 were measured using RT-qPCR, WB, and IF staining. Animal experiments first establish an RSA mice model (CBA/J × DBA/2) and then randomly divide the mice into four groups (n=10): normal pregnancy group, RSA model group, ZYP group, and progesterone capsule group. Observed the changes in embryo absorption rate, pathological morphology of decidual tissue, and ultrastructure of vascular endothelial cells in each group of mice. RT-qPCR, WB, and IF staining methods were used to determine the expression of miR-187, VEGF, VEGF-R1, and VEGF-R2. Results: In vitro, ZYP promoted the viability of PTCs and regulated their cell cycle, and ZYP down-regulated miR-187, up-regulated VEGF, VEGF-R1 and VEGF-R2 levels. miR-187 inhibitor showed the same effects, and further ZYP intervention enhanced the effects. In vivo, ZYP remarkably reduced embryo resorption rates, and improved the pathological morphology of decidual tissues and ultrastructure of vascular endothelial cells. Moreover, ZYP down-regulated miR-187, up-regulated VEGF, VEGF-R1 and VEGF-R2. Conclusion: In summary, ZYP can regulate the expression of VEGF via miR-187, then promote the angiogenesis at the maternal-fetal interface, and playing a therapeutic role in RSA.

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“…According to animal experiments, he found that Tokishakuyakusan, a kind of Japanese Kampo commonly used to treat infertility in Japan, could significantly improve the production of LIF protein and the expression of LIF mRNA in endometrial cells and reduce the decidual dysfunction in rats with implantation disorders, resulting in improving embryo implantation conditions, thus treating infertility [ 78 ]. In addition, Zishen Yutai pill (kidney-nourishing and fetus-raising pill), modified Shoutaiwai recipe (modified ex-longevity fetus recipe), and Bushen Huoxue recipe (kidney-nourishing and blood-promoting recipe) have all been proved by animal experiments to upregulate the expression of integrin αvβ 3 and LIF on endometrium to increase endometrial receptivity and embryo implantation rate [ 79 – 81 ].…”

Section: Methodsmentioning

confidence: 99%

The Treatment of Complementary and Alternative Medicine on Female Infertility Caused by Endometrial Factors

Lin

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Recently, with the development of the social economy, the incidence of infertility has increased year by year. With its complex etiology and diversified syndromes, infertility has become one of the most important diseases that plague the physical and mental health of women of childbearing age worldwide. Endometrial factors as an important part affecting female reproductive capacity, due to which induced repeated abortion and multiple uterine cavity operations occur, can destruct endometrium, failing to provide a normal implantation environment for zygote, thus resulting in infertility. Many patients failed to achieve expected results after receiving conventional treatments such as hormone therapy, assisted reproductive technology (ART), granulocyte colony-stimulating factor (G-CSF) therapy, and cell therapy, then turn to complementary and alternative medicine (CAM) therapies for help. Aiming at clarifying the effectiveness and mechanisms of CAM therapy in the treatment of infertility caused by endometrial factors, our paper systematically searched and studied present related literature on the PubMed, CNKI, and other databases, focusing on the aspects of clinical application and mechanism explorations and highlighting the therapeutic effects of Chinese herbal medicine (CHM), acupuncture, and moxibustion on such diseases. Moreover, this paper also introduces the CAM treatments of traditional Chinese medicine (TCM) retention enema, neuromuscular electrical stimulation (NMES), photobiomodulation therapy, dietary intervention, and other measures for infertility caused by endometrial factors, in order to provide a reference for subsequent basic research and clinical work.

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The potential of Zishen Yutai pills to facilitate endometrial recovery and restore fertility after induced abortion in rats

Cited by 8 publications

References 37 publications

Regulatory effects of Zishen Yutai Pill on endometrial epithelial response in vitro in immunology microenvironment

Regulatory effects of Zishen Yutai Pill on endometrial epithelial response in vitro in immunology microenvironment

Zishen Yutai Pills Promote Angiogenesis at the Maternal-Fetal Interface in Recurrent Spontaneous Abortion Mice by Regulating miR-187/VEGF Axis

The Treatment of Complementary and Alternative Medicine on Female Infertility Caused by Endometrial Factors

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